Publication

Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats

Broekema, M., Harmsen, M. C., van Luyn, M. J. A., Koerts, J. A., Petersen, A. H., van Kooten, T. G., van Goor, H., Navis, G. & Popa, E. R., Jan-2007, In : Journal of the American Society of Nephrology. 18, 1, p. 165-175 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Broekema, M., Harmsen, M. C., van Luyn, M. J. A., Koerts, J. A., Petersen, A. H., van Kooten, T. G., ... Popa, E. R. (2007). Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. Journal of the American Society of Nephrology, 18(1), 165-175. https://doi.org/10.1681/ASN.2005070730

Author

Broekema, Martine ; Harmsen, Martin C. ; van Luyn, Marja J. A. ; Koerts, Jasper A. ; Petersen, Arjen H. ; van Kooten, Theo G. ; van Goor, Harry ; Navis, Gerjan ; Popa, Eliane R. / Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. In: Journal of the American Society of Nephrology. 2007 ; Vol. 18, No. 1. pp. 165-175.

Harvard

Broekema, M, Harmsen, MC, van Luyn, MJA, Koerts, JA, Petersen, AH, van Kooten, TG, van Goor, H, Navis, G & Popa, ER 2007, 'Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats', Journal of the American Society of Nephrology, vol. 18, no. 1, pp. 165-175. https://doi.org/10.1681/ASN.2005070730

Standard

Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. / Broekema, Martine; Harmsen, Martin C.; van Luyn, Marja J. A.; Koerts, Jasper A.; Petersen, Arjen H.; van Kooten, Theo G.; van Goor, Harry; Navis, Gerjan; Popa, Eliane R.

In: Journal of the American Society of Nephrology, Vol. 18, No. 1, 01.2007, p. 165-175.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Broekema M, Harmsen MC, van Luyn MJA, Koerts JA, Petersen AH, van Kooten TG et al. Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. Journal of the American Society of Nephrology. 2007 Jan;18(1):165-175. https://doi.org/10.1681/ASN.2005070730


BibTeX

@article{64d4001194794d39b9471ac0d3751c32,
title = "Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats",
abstract = "Bone marrow-derived cells (BMDC) have been proposed to exert beneficial effects after renal ischemia/reperfusion injury (IRI) by engraftment in the tubular epithelium. However, BMDC can give rise to myofibroblasts and may contribute to fibrosis. BMDC contribution to the renal interstitial myofibroblast population in relation to fibrotic changes after IRI in rats was investigated. A model of unilateral renal IRI (45 min of ischemia) was used in F344 rats that were reconstituted with R26-human placental alkaline phosphatase transgenic BM to quantify BMDC contribution to the renal interstitial myofibroblast population over time. After IRI, transient increases in collagen III transcription and interstitial protein deposition were observed, peaking on days 7 and 28, respectively. Interstitial infiltrates of BMDC and myofibroblasts reached a maximum on day 7 and gradually decreased afterward. Over time, an average of 32{\%} of all interstitial a-smooth muscle actin-positive myofibroblasts coexpressed R26-human placental alkaline phosphatase and, therefore, were derived from the BM. BMD myofibroblasts produced procollagen I protein and therefore were functional. The postischemic kidney environment was profibrotic, as demonstrated by increased transcription of TGF-beta and decreased transcription of bone morphogenic protein-7. TGF-beta protein was present predominantly in interstitial myofibroblasts but not in BMD myofibroblasts. In conclusion, functional BMD myofibroblasts infiltrate in the postischemic renal interstitium and are involved in extracellular matrix production.",
keywords = "ENDOTHELIAL PROGENITOR CELLS, ISCHEMIA-REPERFUSION INJURY, MUSCLE ACTIN EXPRESSION, MESENCHYMAL STEM-CELLS, POSTISCHEMIC KIDNEY, GRANULATION-TISSUE, COLLAGEN-SYNTHESIS, EPITHELIAL-CELLS, FIBROSIS, REPAIR",
author = "Martine Broekema and Harmsen, {Martin C.} and {van Luyn}, {Marja J. A.} and Koerts, {Jasper A.} and Petersen, {Arjen H.} and {van Kooten}, {Theo G.} and {van Goor}, Harry and Gerjan Navis and Popa, {Eliane R.}",
note = "Proceedings Paper 5",
year = "2007",
month = "1",
doi = "10.1681/ASN.2005070730",
language = "English",
volume = "18",
pages = "165--175",
journal = "Journal of the American Society of Nephrology",
issn = "1046-6673",
publisher = "AMER SOC NEPHROLOGY",
number = "1",

}

RIS

TY - JOUR

T1 - Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats

AU - Broekema, Martine

AU - Harmsen, Martin C.

AU - van Luyn, Marja J. A.

AU - Koerts, Jasper A.

AU - Petersen, Arjen H.

AU - van Kooten, Theo G.

AU - van Goor, Harry

AU - Navis, Gerjan

AU - Popa, Eliane R.

N1 - Proceedings Paper 5

PY - 2007/1

Y1 - 2007/1

N2 - Bone marrow-derived cells (BMDC) have been proposed to exert beneficial effects after renal ischemia/reperfusion injury (IRI) by engraftment in the tubular epithelium. However, BMDC can give rise to myofibroblasts and may contribute to fibrosis. BMDC contribution to the renal interstitial myofibroblast population in relation to fibrotic changes after IRI in rats was investigated. A model of unilateral renal IRI (45 min of ischemia) was used in F344 rats that were reconstituted with R26-human placental alkaline phosphatase transgenic BM to quantify BMDC contribution to the renal interstitial myofibroblast population over time. After IRI, transient increases in collagen III transcription and interstitial protein deposition were observed, peaking on days 7 and 28, respectively. Interstitial infiltrates of BMDC and myofibroblasts reached a maximum on day 7 and gradually decreased afterward. Over time, an average of 32% of all interstitial a-smooth muscle actin-positive myofibroblasts coexpressed R26-human placental alkaline phosphatase and, therefore, were derived from the BM. BMD myofibroblasts produced procollagen I protein and therefore were functional. The postischemic kidney environment was profibrotic, as demonstrated by increased transcription of TGF-beta and decreased transcription of bone morphogenic protein-7. TGF-beta protein was present predominantly in interstitial myofibroblasts but not in BMD myofibroblasts. In conclusion, functional BMD myofibroblasts infiltrate in the postischemic renal interstitium and are involved in extracellular matrix production.

AB - Bone marrow-derived cells (BMDC) have been proposed to exert beneficial effects after renal ischemia/reperfusion injury (IRI) by engraftment in the tubular epithelium. However, BMDC can give rise to myofibroblasts and may contribute to fibrosis. BMDC contribution to the renal interstitial myofibroblast population in relation to fibrotic changes after IRI in rats was investigated. A model of unilateral renal IRI (45 min of ischemia) was used in F344 rats that were reconstituted with R26-human placental alkaline phosphatase transgenic BM to quantify BMDC contribution to the renal interstitial myofibroblast population over time. After IRI, transient increases in collagen III transcription and interstitial protein deposition were observed, peaking on days 7 and 28, respectively. Interstitial infiltrates of BMDC and myofibroblasts reached a maximum on day 7 and gradually decreased afterward. Over time, an average of 32% of all interstitial a-smooth muscle actin-positive myofibroblasts coexpressed R26-human placental alkaline phosphatase and, therefore, were derived from the BM. BMD myofibroblasts produced procollagen I protein and therefore were functional. The postischemic kidney environment was profibrotic, as demonstrated by increased transcription of TGF-beta and decreased transcription of bone morphogenic protein-7. TGF-beta protein was present predominantly in interstitial myofibroblasts but not in BMD myofibroblasts. In conclusion, functional BMD myofibroblasts infiltrate in the postischemic renal interstitium and are involved in extracellular matrix production.

KW - ENDOTHELIAL PROGENITOR CELLS

KW - ISCHEMIA-REPERFUSION INJURY

KW - MUSCLE ACTIN EXPRESSION

KW - MESENCHYMAL STEM-CELLS

KW - POSTISCHEMIC KIDNEY

KW - GRANULATION-TISSUE

KW - COLLAGEN-SYNTHESIS

KW - EPITHELIAL-CELLS

KW - FIBROSIS

KW - REPAIR

U2 - 10.1681/ASN.2005070730

DO - 10.1681/ASN.2005070730

M3 - Article

VL - 18

SP - 165

EP - 175

JO - Journal of the American Society of Nephrology

JF - Journal of the American Society of Nephrology

SN - 1046-6673

IS - 1

ER -

ID: 2001100