Publication

Biomaterial Encapsulation Is Enhanced in the Early Stages of the Foreign Body Reaction During Conditional Macrophage Depletion in Transgenic Macrophage Fas-Induced Apoptosis Mice

Bank, R. A., Zandstra, J., Room, H., Petersen, A. H. & van Putten, S. M., 1-Oct-2017, In : Tissue Engineering. Part A. 23, 19-20, p. 1078-1087 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • Biomaterial Encapsulation Is Enhanced in the Early Stages of the Foreign Body Reaction During Conditional Macrophage Depletion in Transgenic Macrophage Fas-Induced Apoptosis Mice

    Final publisher's version, 849 KB, PDF document

DOI

Macrophages are pivotal cells during the foreign body reaction (FBR), as they orchestrate the proinflammatory microenvironment inside and around biomaterials by secretion of inflammatory mediators. Furthermore, they are responsible for the degradation of biomaterials and are thought to instruct the fibroblasts that generate a fibrous capsule around implanted biomaterials. In this study, we investigated the events during the FBR when macrophages are not present. Hexamethylenediisocyanate crosslinked collagen scaffolds were implanted in "Macrophage Fas-Induced Apoptosis'' mice, which allow "on demand'' macrophage depletion. We observed that macrophage depletion completely inhibited inflammatory ingrowth into the scaffolds and resulted in an increased capsule size. Quantitative polymerase chain reaction analysis revealed decreased expression levels of proinflammatory mediators such as TNF alpha and IL1 beta, and increased expression levels of collagens and fibroblast-stimulating growth factors such as EGF, FGF1, FGF2, and TGF alpha. Our results indicate that macrophages are indeed crucial for the generation of a proinflammatory microenvironment inside implanted biomaterials, leading to inflammatory ingrowth. In contrast, macrophages do not appear to be important for the generation of a fibrous capsule around implanted biomaterials. In fact, our data suggest that the macrophages present in the capsule might instruct the surrounding fibroblasts to produce less fibroblast-stimulating factors and less collagens.

Original languageEnglish
Pages (from-to)1078-1087
Number of pages10
JournalTissue Engineering. Part A
Volume23
Issue number19-20
Publication statusPublished - 1-Oct-2017

    Keywords

  • biomaterials, foreign body reaction, macrophages, fibrosis, macrophage depletion, BIOLOGIC SCAFFOLDS, GIANT-CELLS, FIBROSIS, COLLAGEN, EXPRESSION, RESIDENT, REPAIR, DIFFERENTIATION, ERYTHROPOIESIS, PARTICIPATION

View graph of relations

ID: 49612524