Biological in situ characterization of polymeric microbubble contrast agents

Wan, S., Egri, G., Oddo, L., Cerroni, B., Dähne, L., Paradossi, G., Salvati, A., Lynch, I., Dawson, K. A. & Monopoli, M. P., Jun-2016, In : International journal of biochemistry & cell biology. 75, p. 232-243 12 p.

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  • Biological in situ characterization of polymeric microbubble contrast agents

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  • Sha Wan
  • Gabriella Egri
  • Letizia Oddo
  • Barbara Cerroni
  • Lars Dähne
  • Gaio Paradossi
  • Anna Salvati
  • Iseult Lynch
  • Kenneth A. Dawson
  • Marco P. Monopoli

Polymeric microbubbles (MBs) are gas filled particles composed of a thin stabilized polymer shell that have been recently developed as valid contrast agents for the combined use of ultrasonography (US), magnetic resonance imaging (MRI) and single photon emission computer tomography (SPECT) imaging. Due to their buoyancy, the commonly available approaches to study their behaviour in complex media are not easily applicable and their use in modern medicine requires to be fully elucidated. Here we have used for the first time flow cytometry as a new high throughput approach that allows to characterize the MB dispersions, prior and after exposure in different biological media and we have additionally developed a method that allows to characterise the strongly bound proteins adsorbed on the MBs, to fully predict their biological behaviour in biological milieu.

Original languageEnglish
Pages (from-to)232-243
Number of pages12
JournalInternational journal of biochemistry & cell biology
Publication statusPublished - Jun-2016

ID: 30861692