Biological in situ characterization of polymeric microbubble contrast agentsWan, S., Egri, G., Oddo, L., Cerroni, B., Dähne, L., Paradossi, G., Salvati, A., Lynch, I., Dawson, K. A. & Monopoli, M. P., Jun-2016, In : International journal of biochemistry & cell biology. 75, p. 232-243 12 p.
Research output: Contribution to journal › Article › Academic › peer-review
Polymeric microbubbles (MBs) are gas filled particles composed of a thin stabilized polymer shell that have been recently developed as valid contrast agents for the combined use of ultrasonography (US), magnetic resonance imaging (MRI) and single photon emission computer tomography (SPECT) imaging. Due to their buoyancy, the commonly available approaches to study their behaviour in complex media are not easily applicable and their use in modern medicine requires to be fully elucidated. Here we have used for the first time flow cytometry as a new high throughput approach that allows to characterize the MB dispersions, prior and after exposure in different biological media and we have additionally developed a method that allows to characterise the strongly bound proteins adsorbed on the MBs, to fully predict their biological behaviour in biological milieu.
|Number of pages||12|
|Journal||International journal of biochemistry & cell biology|
|Publication status||Published - Jun-2016|