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Bioactive silk coatings reduce the adhesion of Staphylococcus aureus while supporting growth of osteoblast-like cells

Nilebäck, L., Widhe, M., Seijsing, J., Bysell, H., Sharma, P. K. & Hedhammar, M., 17-Jul-2019, In : ACS Applied Materials & Interfaces. 11, 28, p. 24999-25007 9 p.

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  • Bioactive Silk Coatings Reduce the Adhesion of Staphylococcus aureus while Supporting Growth of Osteoblast-like Cells

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DOI

Orthopedic and dental implants are associated with a substantial risk of failure due to biomaterial-associated infections and poor osseointegration. To prevent such outcome, a coating can be applied on the implant to ideally both reduce the risk of bacterial adhesion, and support establishment of osteoblasts. We present a strategy to construct dual-functional silk coatings with such properties. Silk coatings were made from a recombinant partial spider silk protein either alone (silkwt) or fused with a cell-binding motif derived from fibronectin (FN-silk). The biofilm-dispersal enzyme Dispersin B (DspB) and two peptidoglycan degrading endolysins, PlySs2 and SAL-1, were produced recombinantly. A sortase recognition tag (SrtTag) was included to allow site-specific conjugation of each enzyme onto silkwt and FN-silk coatings using an engineered variant of the transpeptidase Sortase A (SrtA*). To evaluate bacterial adhesion on the samples, Staphylococcus aureus was incubated on the coatings, and subsequently subjected to live/dead staining. Fluorescence microscopy revealed a reduced number of bacteria on all silk coatings containing enzymes. Moreover, the bacteria were mobile to a higher degree, indicating a negative influence on the bacterial adhesion. The capability to support mammalian cell interactions was assessed by cultivation of the osteosarcoma cell line U-2 OS on dual-functional surfaces, prepared by conjugating the enzymes onto FN-silk coatings. U-2 OS cells could adhere to silk coatings with enzymes and showed high spreading and viability, demonstrating good cell compatibility.

Original languageEnglish
Pages (from-to)24999-25007
Number of pages9
JournalACS Applied Materials & Interfaces
Volume11
Issue number28
Publication statusPublished - 17-Jul-2019

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