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beta-lactamase producing bacteria in the subgingival microflora of adult patients with periodontitis. A comparison between Spain and The Netherlands

Herrera, D., van Winkelhoff, AJ., Dellemijn-Kippuw, N., Winkel, EG. & Sanz, M., Jul-2000, In : Journal of Clinical Periodontology. 27, 7, p. 520-525 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • D Herrera
  • AJ van Winkelhoff
  • N Dellemijn-Kippuw
  • EG Winkel
  • M Sanz

Background/aims: Countries with a high per capita antibiotic use frequently demonstrate a high level of drug resistance. The aim of this study was to compare the prevalence and levels of beta-lactamase producing bacteria in the subgingival microflora in adult patients with periodontitis in Spain and The Netherlands, and to characterise beta-lactamase producing bacteria in both patient samples.

Method: Patients with moderate to severe periodontitis were consecutively selected and asked to report on: current systemic disorders and medications, history of use of antibiotics, and smoking habits. Clinical variables included probing pocket depth, clinical attachment level, plaque, bleeding on probing, and suppuration. Pooled subgingival samples of 4 selected sites were anaerobically cultured in blood agar plates with and without amoxicillin, and amoxicillin/clavulanate. Bacterial colonies growing on amoxicillin plates but not on amoxicillin/clavulanate plates were tested for beta-lactamase production. beta-lactamase producing bacteria were isolated and identified.

Results: 31 patients were studied in the Spanish group and 30 in the Dutch group. Comparable mean gender and ages were found. Evaluation of previous antibiotic use revealed that, in the previous 12 months, 54.8% of patients in the Spanish group and 10% in the Dutch group reported antibiotic use (p

Conclusions: A high prevalence of beta-lactamase producing bacteria has been evaluated in two distinct populations, belonging to two European countries with clear differences in antibiotic usage policy. A higher prevalence and a more complex beta-lactamase producing microflora, were found in the Spanish group, associated with a higher antibiotic consumption. This study shows that a higher use of beta-lactam antibiotics is reflected in the % of beta-lactamase producing bacteria in the subgingival microflora of patients with periodontitis. This information may be important in the treatment of severe periodontitis.

Original languageEnglish
Pages (from-to)520-525
Number of pages6
JournalJournal of Clinical Periodontology
Volume27
Issue number7
Publication statusPublished - Jul-2000

    Keywords

  • adult periodontitis, beta-lactamase, bacterial resistance, Spain, The Netherlands, amoxicillin, ANTIBIOTIC-RESISTANCE, COLLABORATIVE SURVEILLANCE, PATHOGENS, EUROPE, SUSCEPTIBILITY, BACTEROIDES, INFLUENZAE, PREVALENCE, PATTERNS, TRENDS

ID: 13929310