Beneficial Effects of Alternate Dietary Regimen on Liver Inflammation, Atherosclerosis and Renal ActivationWielinga, P. Y., Yakala, G. K., Heeringa, P., Kleemann, R. & Kooistra, T., 31-Mar-2011, In : PLoS ONE. 6, 3, 10 p., 18432.
Research output: Contribution to journal › Article › Academic › peer-review
Background: Alternate day calorie restriction (CR) has been shown to be almost as beneficial as daily CR. The question arises whether this concept is also applicable to alternating dietary composition.
Objective: To seek evidence that alternating high cholesterol (HC) - cholesterol-free (CON) Western diet can effectively diminish hepatic and renal inflammation and cardiovascular risk factors as compared with daily HC-supplemented Western diet.
Design: Four groups of ApoE*3Leiden mice, a humanized model for atherosclerosis, were subjected to different feeding treatments for 16 weeks. Mice were fed CON diet; CON diet with 1% w/w cholesterol (HC); alternate (ALT) diet regimen of CON (4 days) and HC (3 days); or CON diet supplemented with 0.43% (w/w) cholesterol (MC), with overall dietary cholesterol intake equal to ALT. Plasma was analyzed for cardiovascular risk factors, aorta for atherosclerotic lesion formation, and liver and kidney for inflammation.
Results: ALT diet but not MC was almost as effective as daily CON feeding in preventing disease development. Compared to HC, the ALT group showed 62% lower hepatic nuclear factor kappa B (NF-kappa B) activity (P <0.001), a reduction of the circulating inflammatory markers E-selectin (-20%; P <0.05), vascular cell adhesion molecule 1 (VCAM-1; -15%; P <0.05) and Serum Amyloid A (SAA; -31%; P <0.05), smaller atherosclerotic lesion sizes (-51%; 46497 +/- 10791 mu m(2) vs. 94664 +/- 16470 mu m(2); P <0.05) and diminished renal expression of specific inflammation and activation markers (VCAM-1, -27%; P <0.05; monocyte chemotactic protein-1 (MCP-1); -37%; P <0.01).
Conclusion: Alternate HC-CON feeding reproduced most of the beneficial effects of daily cholesterol-free diet, including strongly diminished hepatic, vascular and renal activation and inflammation; also atherosclerosis was reduced by half as compared to HC, albeit still higher compared to the CON group.
|Number of pages||10|
|Publication status||Published - 31-Mar-2011|
- CALORIE RESTRICTION, TRANSGENIC MICE, CHOLESTEROL, DISEASE