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Autoantibodies to myeloperoxidase aggravate mild anti-glomerular-basement-membrane-mediated glomerular injury in the rat

Heeringa, P., Brouwer, E., Klok, PA., Huitema, MG., vandenBorn, J., Kallenberg, CGM. & Weening, J. J., Nov-1996, In : American Journal of Pathology. 149, 5, p. 1695 - 1706 12 p.

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Autoantibodies to myeloperoxidase (MPO) are present in sera from patients with various forms of vasculitis-associated glomerulonephritis. Evidence for a pathogenic role of anti-MPO antibodies has been provided mainly by in vitro studies. We studied the pathogenic role of autoantibodies to MPO in a rat model of mild immune-mediated glomerular injury. Brown Norway rats were immunized with human MPO in complete Freund's adjuvant or with complete Freund's adjuvant alone. At 2 weeks after immunization, rats had developed antibodies to human and rat MPO as detected by indirect immunofluorescence, enzyme-linked immunosorbent assay, and immunoprecipitation. At this time point, rats were intravenously injected with a subnephritogenic dose of 150 mu g of rabbit anti-rat GBM. Rats were sacrificed at 4 hours, 24 hours, 4 days, and 10 days after antibody administration. Control immunized rats developed mild glomerulonephritis characterized by slight proteinuria at day 10 (14.8 +/- 8.1 mg/24 hours) and moderate intraglomerular accumulation of ED1(+) macrophages. Crescent formation, tuft necrosis, and tubular atrophy were not observed in those rats. In contrast, rats immunized with MPO developed severe glomerulonephritis characterized by the early occurrence of severe hematuria, marked proteinuria at day 10 (76.2 +/- 18.2 mg/24 hours), and massive glomerular deposition of fibrin. Complement and rat IgG were present in insudative lesions, but no linear pattern along the glomerular capillary wall was observed. By light microscopy, severe glomerular lesions were found at day 10 consisting of crescent formation and fibrinoid necrosis of capillary loops. In the interstitium, tubular necrosis and atrophy and marked interstitial mononuclear infiltration were found. In conclusion, autoantibodies to MPO severely aggravate subclinical anti-GBM disease demonstrating their in vivo pathogenic potential.

Original languageEnglish
Pages (from-to)1695 - 1706
Number of pages12
JournalAmerican Journal of Pathology
Volume149
Issue number5
Publication statusPublished - Nov-1996

    Keywords

  • RAPIDLY PROGRESSIVE GLOMERULONEPHRITIS, ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES, HUMAN ENDOTHELIAL-CELLS, SYSTEMIC VASCULITIS, HUMAN-MONOCYTES, ANTIMYELOPEROXIDASE ANTIBODIES, WEGENERS GRANULOMATOSIS, MERCURIC-CHLORIDE, HUMAN-NEUTROPHILS, OXYGEN RADICALS

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