Publication

Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter

Boelen, P., Obernosterer, I., Vink, A. A. & Buma, A. G. J., Jan-1999, In : Photochemistry and Photobiology. 69, 1, p. 34 - 40 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Boelen, P., Obernosterer, I., Vink, A. A., & Buma, A. G. J. (1999). Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter. Photochemistry and Photobiology, 69(1), 34 - 40.

Author

Boelen, P. ; Obernosterer, I ; Vink, A.A ; Buma, A.G.J. / Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter. In: Photochemistry and Photobiology. 1999 ; Vol. 69, No. 1. pp. 34 - 40.

Harvard

Boelen, P, Obernosterer, I, Vink, AA & Buma, AGJ 1999, 'Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter', Photochemistry and Photobiology, vol. 69, no. 1, pp. 34 - 40.

Standard

Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter. / Boelen, P.; Obernosterer, I; Vink, A.A; Buma, A.G.J.

In: Photochemistry and Photobiology, Vol. 69, No. 1, 01.1999, p. 34 - 40.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Boelen P, Obernosterer I, Vink AA, Buma AGJ. Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter. Photochemistry and Photobiology. 1999 Jan;69(1):34 - 40.


BibTeX

@article{a6301915dd52455986a7c710c3b55e6b,
title = "Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter",
abstract = "A biochemical dosimeter was developed to study the attenuation of biologically effective UV radiation in marine tropical waters. Small quartz vials were used containing a solution of DNA molecules; the vials were incubated at discrete water depths. Subsequently, DNA damage was determined in these samples, using an antibody directed against thymine dimers followed by chemiluminescent detection. Measurements of DNA damage were compared with calculated biologically effective doses, as derived from spectroradiometer measurements. The bio-dosimeter was found to be a reliable and easy tool to determine levels of harmful UV radiation in marine waters. The highest attenuation coefficient (1.60 m(-1)) measured with the biochemical dosimeter was found in eutrophic waters, at a coastal station off Curabcao, Netherlands Antilles. At the other stations attenuation coefficients ranged from 0.18 m(-1) in central Atlantic waters to 0.43 m(-1) close to the Curabcao coast line. Latter results indicate that biologically effective UV radiation may easily reach ecologically significant depths, e.g. coral reef communities.",
keywords = "SOLAR ULTRAVIOLET-RADIATION, BACTERIOPLANKTON, PHYTOPLANKTON, PENETRATION, PHOTODAMAGE, CELLS",
author = "P. Boelen and I Obernosterer and A.A Vink and A.G.J. Buma",
year = "1999",
month = jan,
language = "English",
volume = "69",
pages = "34 -- 40",
journal = "Photochemistry and Photobiology",
issn = "1751-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Attenuation of biologically effective UV radiation in tropical Atlantic waters measured with a biochemical DNA dosimeter

AU - Boelen, P.

AU - Obernosterer, I

AU - Vink, A.A

AU - Buma, A.G.J.

PY - 1999/1

Y1 - 1999/1

N2 - A biochemical dosimeter was developed to study the attenuation of biologically effective UV radiation in marine tropical waters. Small quartz vials were used containing a solution of DNA molecules; the vials were incubated at discrete water depths. Subsequently, DNA damage was determined in these samples, using an antibody directed against thymine dimers followed by chemiluminescent detection. Measurements of DNA damage were compared with calculated biologically effective doses, as derived from spectroradiometer measurements. The bio-dosimeter was found to be a reliable and easy tool to determine levels of harmful UV radiation in marine waters. The highest attenuation coefficient (1.60 m(-1)) measured with the biochemical dosimeter was found in eutrophic waters, at a coastal station off Curabcao, Netherlands Antilles. At the other stations attenuation coefficients ranged from 0.18 m(-1) in central Atlantic waters to 0.43 m(-1) close to the Curabcao coast line. Latter results indicate that biologically effective UV radiation may easily reach ecologically significant depths, e.g. coral reef communities.

AB - A biochemical dosimeter was developed to study the attenuation of biologically effective UV radiation in marine tropical waters. Small quartz vials were used containing a solution of DNA molecules; the vials were incubated at discrete water depths. Subsequently, DNA damage was determined in these samples, using an antibody directed against thymine dimers followed by chemiluminescent detection. Measurements of DNA damage were compared with calculated biologically effective doses, as derived from spectroradiometer measurements. The bio-dosimeter was found to be a reliable and easy tool to determine levels of harmful UV radiation in marine waters. The highest attenuation coefficient (1.60 m(-1)) measured with the biochemical dosimeter was found in eutrophic waters, at a coastal station off Curabcao, Netherlands Antilles. At the other stations attenuation coefficients ranged from 0.18 m(-1) in central Atlantic waters to 0.43 m(-1) close to the Curabcao coast line. Latter results indicate that biologically effective UV radiation may easily reach ecologically significant depths, e.g. coral reef communities.

KW - SOLAR ULTRAVIOLET-RADIATION

KW - BACTERIOPLANKTON

KW - PHYTOPLANKTON

KW - PENETRATION

KW - PHOTODAMAGE

KW - CELLS

M3 - Article

VL - 69

SP - 34

EP - 40

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 1751-1097

IS - 1

ER -

ID: 746962