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Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha

Leão-Helder, A. N., Krikken, A. M., Gellissen, G., van der Klei, I. J., Veenhuis, M. & Kiel, J. A. K. W., 2004, In : FEBS Letters. 577, 3, p. 491 - 495 5 p.

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DOI

ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual location in the cytosol and in peri-vacuolar dots. We demonstrate that Hp-Atg21p is essential for two separate modes of peroxisome degradation, namely glucose-induced macropexophagy and nitrogen limitation-induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.

Original languageEnglish
Pages (from-to)491 - 495
Number of pages5
JournalFEBS Letters
Volume577
Issue number3
Publication statusPublished - 2004

    Keywords

  • Autophagy, Cytosol, Fungal Proteins, Genes, Fungal, Glucose, Green Fluorescent Proteins, Microscopy, Fluorescence, Mutation, Peroxisomes, Pichia, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Vacuoles

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