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Asymmetric inheritance of centrosome-associated primary cilium membrane directs ciliogenesis after cell division

Paridaen, J. T. M. L., Wilsch-Bräuninger, M. & Huttner, W. B., 10-Oct-2013, In : Cell. 155, 2, p. 333-344 12 p.

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  • Asymmetric Inheritance of Centrosome-Associated Primary Cilium Membrane Directs Ciliogenesis after Cell Division

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DOI

Primary cilia are key sensory organelles that are thought to be disassembled prior to mitosis. Inheritance of the mother centriole, which nucleates the primary cilium, in relation to asymmetric daughter cell behavior has previously been studied. However, the fate of the ciliary membrane upon cell division is unknown. Here, we followed the ciliary membrane in dividing embryonic neocortical stem cells and cultured cells. Ciliary membrane attached to the mother centriole was endocytosed at mitosis onset, persisted through mitosis at one spindle pole, and was asymmetrically inherited by one daughter cell, which retained stem cell character. This daughter re-established a primary cilium harboring an activated signal transducer earlier than the noninheriting daughter. Centrosomal association of ciliary membrane in dividing neural stem cells decreased at late neurogenesis when these cells differentiate. Our data imply that centrosome-associated ciliary membrane acts as a determinant for the temporal-spatial control of ciliogenesis.

Original languageEnglish
Pages (from-to)333-344
Number of pages12
JournalCell
Volume155
Issue number2
Publication statusPublished - 10-Oct-2013
Externally publishedYes

    Keywords

  • ADP-Ribosylation Factors, Animals, Cell Division, Centrioles, Centrosome, Cilia, HEK293 Cells, Humans, Mice, Mitosis, Neural Stem Cells, Journal Article, Research Support, Non-U.S. Gov't

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