Publication

Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication

Pereira, C. F., Paridaen, J. T. M. L., Rutten, K., Huigen, M. C. D. G., van de Bovenkamp, M., Middel, J., Beerens, N., Berkhout, B., Schuurman, R., Marnett, L. J., Verhoef, J. & Nottet, H. S. L. M., May-2003, In : Antiviral Research. 58, 3, p. 253-63 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Pereira, C. F., Paridaen, J. T. M. L., Rutten, K., Huigen, M. C. D. G., van de Bovenkamp, M., Middel, J., ... Nottet, H. S. L. M. (2003). Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication. Antiviral Research, 58(3), 253-63. https://doi.org/10.1016/S0166-3542(03)00006-8

Author

Pereira, Cândida F ; Paridaen, Judith T M L ; Rutten, Karla ; Huigen, Marleen C D G ; van de Bovenkamp, Marja ; Middel, Jeena ; Beerens, Nancy ; Berkhout, Ben ; Schuurman, Rob ; Marnett, Lawrence J ; Verhoef, Jan ; Nottet, Hans S L M. / Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication. In: Antiviral Research. 2003 ; Vol. 58, No. 3. pp. 253-63.

Harvard

Pereira, CF, Paridaen, JTML, Rutten, K, Huigen, MCDG, van de Bovenkamp, M, Middel, J, Beerens, N, Berkhout, B, Schuurman, R, Marnett, LJ, Verhoef, J & Nottet, HSLM 2003, 'Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication', Antiviral Research, vol. 58, no. 3, pp. 253-63. https://doi.org/10.1016/S0166-3542(03)00006-8

Standard

Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication. / Pereira, Cândida F; Paridaen, Judith T M L; Rutten, Karla; Huigen, Marleen C D G; van de Bovenkamp, Marja; Middel, Jeena; Beerens, Nancy; Berkhout, Ben; Schuurman, Rob; Marnett, Lawrence J; Verhoef, Jan; Nottet, Hans S L M.

In: Antiviral Research, Vol. 58, No. 3, 05.2003, p. 253-63.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Pereira CF, Paridaen JTML, Rutten K, Huigen MCDG, van de Bovenkamp M, Middel J et al. Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication. Antiviral Research. 2003 May;58(3):253-63. https://doi.org/10.1016/S0166-3542(03)00006-8


BibTeX

@article{c1fe6ad5341c424ab5c3ebd3eb06be78,
title = "Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication",
abstract = "Some anti-inflammatory molecules are also known to possess anti-human immunodeficiency virus (HIV) activity. We found that o-(acetoxyphenyl)hept-2-ynyl sulfide (APHS), a recently synthesized non-steroidal anti-inflammatory molecule can inhibit HIV-1 replication. The aim of this study was to clarify the mechanism of action of APHS. When administered during the first steps of the infection, APHS was capable of inhibiting the replication of several HIV-1 strains (macrophage-tropic and/or lymphocytotropic) in a dose-dependent manner in both peripheral blood mononuclear cells (PBMC), monocyte-derived macrophages and peripheral blood lymphocytes with 50{\%} inhibitory concentration values of approximately 10 microM. The 50{\%} toxic concentration of APHS varied between 100 and 200 microM in the different primary cells tested. APHS did not affect HIV-1 replication once the provirus was already inserted into the cellular genome. APHS also did not inhibit HIV-1 entry into the host cells as determined by quantification of gag RNA inside PBMC 2h after infection. However, APHS did inhibit gag DNA synthesis during reverse transcription in primary cells, which indicates that APHS may target the reverse transcription process.",
keywords = "Acetylene, Alkynes, Anti-Inflammatory Agents, Non-Steroidal, Aspirin, Cell Line, Cells, Cultured, DNA, Viral, HIV-1, Humans, Leukocytes, Mononuclear, Lymphocytes, Macrophages, Monocytes, Polymerase Chain Reaction, RNA, Viral, Sulfides, Taq Polymerase, Transcription, Genetic, Virus Replication, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.",
author = "Pereira, {C{\^a}ndida F} and Paridaen, {Judith T M L} and Karla Rutten and Huigen, {Marleen C D G} and {van de Bovenkamp}, Marja and Jeena Middel and Nancy Beerens and Ben Berkhout and Rob Schuurman and Marnett, {Lawrence J} and Jan Verhoef and Nottet, {Hans S L M}",
year = "2003",
month = "5",
doi = "10.1016/S0166-3542(03)00006-8",
language = "English",
volume = "58",
pages = "253--63",
journal = "Antiviral Research",
issn = "0166-3542",
publisher = "ELSEVIER SCIENCE BV",
number = "3",

}

RIS

TY - JOUR

T1 - Aspirin-like molecules that inhibit human immunodeficiency virus 1 replication

AU - Pereira, Cândida F

AU - Paridaen, Judith T M L

AU - Rutten, Karla

AU - Huigen, Marleen C D G

AU - van de Bovenkamp, Marja

AU - Middel, Jeena

AU - Beerens, Nancy

AU - Berkhout, Ben

AU - Schuurman, Rob

AU - Marnett, Lawrence J

AU - Verhoef, Jan

AU - Nottet, Hans S L M

PY - 2003/5

Y1 - 2003/5

N2 - Some anti-inflammatory molecules are also known to possess anti-human immunodeficiency virus (HIV) activity. We found that o-(acetoxyphenyl)hept-2-ynyl sulfide (APHS), a recently synthesized non-steroidal anti-inflammatory molecule can inhibit HIV-1 replication. The aim of this study was to clarify the mechanism of action of APHS. When administered during the first steps of the infection, APHS was capable of inhibiting the replication of several HIV-1 strains (macrophage-tropic and/or lymphocytotropic) in a dose-dependent manner in both peripheral blood mononuclear cells (PBMC), monocyte-derived macrophages and peripheral blood lymphocytes with 50% inhibitory concentration values of approximately 10 microM. The 50% toxic concentration of APHS varied between 100 and 200 microM in the different primary cells tested. APHS did not affect HIV-1 replication once the provirus was already inserted into the cellular genome. APHS also did not inhibit HIV-1 entry into the host cells as determined by quantification of gag RNA inside PBMC 2h after infection. However, APHS did inhibit gag DNA synthesis during reverse transcription in primary cells, which indicates that APHS may target the reverse transcription process.

AB - Some anti-inflammatory molecules are also known to possess anti-human immunodeficiency virus (HIV) activity. We found that o-(acetoxyphenyl)hept-2-ynyl sulfide (APHS), a recently synthesized non-steroidal anti-inflammatory molecule can inhibit HIV-1 replication. The aim of this study was to clarify the mechanism of action of APHS. When administered during the first steps of the infection, APHS was capable of inhibiting the replication of several HIV-1 strains (macrophage-tropic and/or lymphocytotropic) in a dose-dependent manner in both peripheral blood mononuclear cells (PBMC), monocyte-derived macrophages and peripheral blood lymphocytes with 50% inhibitory concentration values of approximately 10 microM. The 50% toxic concentration of APHS varied between 100 and 200 microM in the different primary cells tested. APHS did not affect HIV-1 replication once the provirus was already inserted into the cellular genome. APHS also did not inhibit HIV-1 entry into the host cells as determined by quantification of gag RNA inside PBMC 2h after infection. However, APHS did inhibit gag DNA synthesis during reverse transcription in primary cells, which indicates that APHS may target the reverse transcription process.

KW - Acetylene

KW - Alkynes

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Aspirin

KW - Cell Line

KW - Cells, Cultured

KW - DNA, Viral

KW - HIV-1

KW - Humans

KW - Leukocytes, Mononuclear

KW - Lymphocytes

KW - Macrophages

KW - Monocytes

KW - Polymerase Chain Reaction

KW - RNA, Viral

KW - Sulfides

KW - Taq Polymerase

KW - Transcription, Genetic

KW - Virus Replication

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

U2 - 10.1016/S0166-3542(03)00006-8

DO - 10.1016/S0166-3542(03)00006-8

M3 - Article

VL - 58

SP - 253

EP - 263

JO - Antiviral Research

JF - Antiviral Research

SN - 0166-3542

IS - 3

ER -

ID: 53430831