Publication

Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells

Teteloshvili, N., Smigielska-Czepiel, K., Yuan, Y., Seitz, A., de Jong, D., Rutgers, B., Jellema, P., van der Lei, R. J., Slezak-Prochazka, I., Brouwer, E., Boots, A. M. H., Kroesen, B-J., van den Berg, A. & Kluiver, J., Feb-2017, In : Febs Journal. 284, 4, p. 555-567 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Teteloshvili, N., Smigielska-Czepiel, K., Yuan, Y., Seitz, A., de Jong, D., Rutgers, B., ... Kluiver, J. (2017). Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells. Febs Journal, 284(4), 555-567. https://doi.org/10.1111/febs.14011

Author

Teteloshvili, Nato ; Smigielska-Czepiel, Katarzyna ; Yuan, Ye ; Seitz, Annika ; de Jong, Debora ; Rutgers, Bea ; Jellema, Pytrick ; van der Lei, Roelof Jan ; Slezak-Prochazka, Izabella ; Brouwer, Elisabeth ; Boots, Annemieke M. H. ; Kroesen, Bart-Jan ; van den Berg, Anke ; Kluiver, Joost. / Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells. In: Febs Journal. 2017 ; Vol. 284, No. 4. pp. 555-567.

Harvard

Teteloshvili, N, Smigielska-Czepiel, K, Yuan, Y, Seitz, A, de Jong, D, Rutgers, B, Jellema, P, van der Lei, RJ, Slezak-Prochazka, I, Brouwer, E, Boots, AMH, Kroesen, B-J, van den Berg, A & Kluiver, J 2017, 'Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells' Febs Journal, vol. 284, no. 4, pp. 555-567. https://doi.org/10.1111/febs.14011

Standard

Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells. / Teteloshvili, Nato; Smigielska-Czepiel, Katarzyna; Yuan, Ye; Seitz, Annika; de Jong, Debora; Rutgers, Bea; Jellema, Pytrick; van der Lei, Roelof Jan; Slezak-Prochazka, Izabella; Brouwer, Elisabeth; Boots, Annemieke M. H.; Kroesen, Bart-Jan; van den Berg, Anke; Kluiver, Joost.

In: Febs Journal, Vol. 284, No. 4, 02.2017, p. 555-567.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Teteloshvili N, Smigielska-Czepiel K, Yuan Y, Seitz A, de Jong D, Rutgers B et al. Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells. Febs Journal. 2017 Feb;284(4):555-567. https://doi.org/10.1111/febs.14011


BibTeX

@article{5bf87551f7aa43ed83cd973663c41d76,
title = "Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells",
abstract = "MicroRNA (miR)-21 is an important suppressor of T-cell apoptosis that is also overexpressed in many types of cancers. The exact mechanisms underlying the antiapoptotic effects of miR-21 are not well understood. In this study, we used the Jurkat T-cell line as a model to identify apoptosis-associated miR-21 target genes. We showed that expression of miR-21 rapidly increases upon alpha CD3/alpha CD28 activation of Jurkat cells. Inhibition of miR-21 reduced cell growth which could be explained by an increase in apoptosis. MicroRNA target gene identification by AGO2 RNA-immunoprecipitation followed by gene expression microarray (RIP-Chip) resulted in the identification of 72 predicted miR-21 target genes that were at least twofold enriched in the AGO2-IP fraction of miR-21 overexpressing cells. Of these, 71 were at least twofold more enriched in the AGO2-IP fraction of miR-21 overexpressing cells as compared to AGO2-IP fraction of control cells. The target gene for which the AGO2-IP enrichment was most prominently increased upon miR-21 overexpression was the proapoptotic protein LATS1. Luciferase reporter assays and western blot analysis confirmed targeting of LATS1 by miR-21. qRT-PCR analysis in primary T cells showed an inverse expression pattern between LATS1 transcript levels and miR-21 upon T-cell stimulation. Finally, LATS1 knockdown partially rescued the miR-21 inhibition-induced impaired cell growth. Collectively, these data identify LATS1 as a miR-21 target important for the antiapoptotic function of miR-21 in T cells and likely also in many types of cancer.",
keywords = "AGO2-RIP, apoptosis, LATS1, miR-21, T cells, BREAST-CANCER CELLS, MICRORNA-21 TARGETS, MIRNA EXPRESSION, APOPTOSIS, GENE, INVASION, GROWTH, SIGNATURES, LYMPHOMA",
author = "Nato Teteloshvili and Katarzyna Smigielska-Czepiel and Ye Yuan and Annika Seitz and {de Jong}, Debora and Bea Rutgers and Pytrick Jellema and {van der Lei}, {Roelof Jan} and Izabella Slezak-Prochazka and Elisabeth Brouwer and Boots, {Annemieke M. H.} and Bart-Jan Kroesen and {van den Berg}, Anke and Joost Kluiver",
year = "2017",
month = "2",
doi = "10.1111/febs.14011",
language = "English",
volume = "284",
pages = "555--567",
journal = "Febs Journal",
issn = "1742-464X",
publisher = "NLM (Medline)",
number = "4",

}

RIS

TY - JOUR

T1 - Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells

AU - Teteloshvili, Nato

AU - Smigielska-Czepiel, Katarzyna

AU - Yuan, Ye

AU - Seitz, Annika

AU - de Jong, Debora

AU - Rutgers, Bea

AU - Jellema, Pytrick

AU - van der Lei, Roelof Jan

AU - Slezak-Prochazka, Izabella

AU - Brouwer, Elisabeth

AU - Boots, Annemieke M. H.

AU - Kroesen, Bart-Jan

AU - van den Berg, Anke

AU - Kluiver, Joost

PY - 2017/2

Y1 - 2017/2

N2 - MicroRNA (miR)-21 is an important suppressor of T-cell apoptosis that is also overexpressed in many types of cancers. The exact mechanisms underlying the antiapoptotic effects of miR-21 are not well understood. In this study, we used the Jurkat T-cell line as a model to identify apoptosis-associated miR-21 target genes. We showed that expression of miR-21 rapidly increases upon alpha CD3/alpha CD28 activation of Jurkat cells. Inhibition of miR-21 reduced cell growth which could be explained by an increase in apoptosis. MicroRNA target gene identification by AGO2 RNA-immunoprecipitation followed by gene expression microarray (RIP-Chip) resulted in the identification of 72 predicted miR-21 target genes that were at least twofold enriched in the AGO2-IP fraction of miR-21 overexpressing cells. Of these, 71 were at least twofold more enriched in the AGO2-IP fraction of miR-21 overexpressing cells as compared to AGO2-IP fraction of control cells. The target gene for which the AGO2-IP enrichment was most prominently increased upon miR-21 overexpression was the proapoptotic protein LATS1. Luciferase reporter assays and western blot analysis confirmed targeting of LATS1 by miR-21. qRT-PCR analysis in primary T cells showed an inverse expression pattern between LATS1 transcript levels and miR-21 upon T-cell stimulation. Finally, LATS1 knockdown partially rescued the miR-21 inhibition-induced impaired cell growth. Collectively, these data identify LATS1 as a miR-21 target important for the antiapoptotic function of miR-21 in T cells and likely also in many types of cancer.

AB - MicroRNA (miR)-21 is an important suppressor of T-cell apoptosis that is also overexpressed in many types of cancers. The exact mechanisms underlying the antiapoptotic effects of miR-21 are not well understood. In this study, we used the Jurkat T-cell line as a model to identify apoptosis-associated miR-21 target genes. We showed that expression of miR-21 rapidly increases upon alpha CD3/alpha CD28 activation of Jurkat cells. Inhibition of miR-21 reduced cell growth which could be explained by an increase in apoptosis. MicroRNA target gene identification by AGO2 RNA-immunoprecipitation followed by gene expression microarray (RIP-Chip) resulted in the identification of 72 predicted miR-21 target genes that were at least twofold enriched in the AGO2-IP fraction of miR-21 overexpressing cells. Of these, 71 were at least twofold more enriched in the AGO2-IP fraction of miR-21 overexpressing cells as compared to AGO2-IP fraction of control cells. The target gene for which the AGO2-IP enrichment was most prominently increased upon miR-21 overexpression was the proapoptotic protein LATS1. Luciferase reporter assays and western blot analysis confirmed targeting of LATS1 by miR-21. qRT-PCR analysis in primary T cells showed an inverse expression pattern between LATS1 transcript levels and miR-21 upon T-cell stimulation. Finally, LATS1 knockdown partially rescued the miR-21 inhibition-induced impaired cell growth. Collectively, these data identify LATS1 as a miR-21 target important for the antiapoptotic function of miR-21 in T cells and likely also in many types of cancer.

KW - AGO2-RIP

KW - apoptosis

KW - LATS1

KW - miR-21

KW - T cells

KW - BREAST-CANCER CELLS

KW - MICRORNA-21 TARGETS

KW - MIRNA EXPRESSION

KW - APOPTOSIS

KW - GENE

KW - INVASION

KW - GROWTH

KW - SIGNATURES

KW - LYMPHOMA

U2 - 10.1111/febs.14011

DO - 10.1111/febs.14011

M3 - Article

VL - 284

SP - 555

EP - 567

JO - Febs Journal

JF - Febs Journal

SN - 1742-464X

IS - 4

ER -

ID: 41120571