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Apparatus and method for parallel collection and analysis of the proteome and complex compositions

Oroskar, A. A., Oroskar, A. R., LaCava, J. & Rout, M. P., 21-Jul-2015, Patent No. USPTO 9084994 , 9-Sep-2011

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  • Apparatus and method for parallel collection and analysis of the proteome and complex compositions

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  • Asha A. Oroskar (Inventor)
  • Anil R. Oroskar (Inventor)
  • J. LaCava (Inventor)
  • Michael P. Rout (Inventor)
This invention relates to a kit and a method for the collection and analysis of complex protein mixtures. More particularly, the invention relates to a kit comprising a single barrel filtration well or a multi-well filtration plate wherein each well comprises an upper filtration zone; a lower filtration zone; a conical flow director zone; and, an elution tip, wherein the upper filtration zone and the lower filtration zone are separated by a retainer ring disposed within the lower filtration zone. The upper filtration zone comprises an upper collection zone, a sponge zone, and a deep bed filtration zone; and, the lower filtration zone comprises the retainer ring, a supported hydrophilic membrane and a lower bed filtration media. When used with an array of selected buffer solutions, the multi-well filtration plate can provide accurate, automated, high-throughput protein analysis by affinity chromatography.
Original languageEnglish
Patent numberUSPTO 9084994
Filing date09/09/2011
Publication statusPublished - 21-Jul-2015
Externally publishedYes

ID: 117222390