Apparatus and method for parallel collection and analysis of the proteome and complex compositionsOroskar, A. A., Oroskar, A. R., LaCava, J. & Rout, M. P., 21-Jul-2015, Patent No. USPTO 9084994 , 9-Sep-2011
Research output: Patent
This invention relates to a kit and a method for the collection and analysis of complex protein mixtures. More particularly, the invention relates to a kit comprising a single barrel filtration well or a multi-well filtration plate wherein each well comprises an upper filtration zone; a lower filtration zone; a conical flow director zone; and, an elution tip, wherein the upper filtration zone and the lower filtration zone are separated by a retainer ring disposed within the lower filtration zone. The upper filtration zone comprises an upper collection zone, a sponge zone, and a deep bed filtration zone; and, the lower filtration zone comprises the retainer ring, a supported hydrophilic membrane and a lower bed filtration media. When used with an array of selected buffer solutions, the multi-well filtration plate can provide accurate, automated, high-throughput protein analysis by affinity chromatography.
|Patent number||USPTO 9084994|
|Publication status||Published - 21-Jul-2015|