Publication

Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats

Rebolledo Acevedo, R., Van Erp, A. C., Ottens, P. J., Wiersema-Buist, J., Leuvenink, H. G. D. & Romanque, P., 5-Oct-2015, In : PLoS ONE. 10, 10, 14 p., e0138749.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Rebolledo Acevedo, R., Van Erp, A. C., Ottens, P. J., Wiersema-Buist, J., Leuvenink, H. G. D., & Romanque, P. (2015). Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats. PLoS ONE, 10(10), [e0138749]. https://doi.org/10.1371/journal.pone.0138749

Author

Rebolledo Acevedo, Rolando ; Van Erp, Anne C. ; Ottens, Petra J. ; Wiersema-Buist, Jantje ; Leuvenink, Henri G. D. ; Romanque, Pamela. / Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats. In: PLoS ONE. 2015 ; Vol. 10, No. 10.

Harvard

Rebolledo Acevedo, R, Van Erp, AC, Ottens, PJ, Wiersema-Buist, J, Leuvenink, HGD & Romanque, P 2015, 'Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats', PLoS ONE, vol. 10, no. 10, e0138749. https://doi.org/10.1371/journal.pone.0138749

Standard

Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats. / Rebolledo Acevedo, Rolando; Van Erp, Anne C.; Ottens, Petra J.; Wiersema-Buist, Jantje; Leuvenink, Henri G. D.; Romanque, Pamela.

In: PLoS ONE, Vol. 10, No. 10, e0138749, 05.10.2015.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Rebolledo Acevedo R, Van Erp AC, Ottens PJ, Wiersema-Buist J, Leuvenink HGD, Romanque P. Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats. PLoS ONE. 2015 Oct 5;10(10). e0138749. https://doi.org/10.1371/journal.pone.0138749


BibTeX

@article{be726fe6c995473c97c402beade2f721,
title = "Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats",
abstract = "BackgroundThyroid hormone treatment in brain-dead organ donors has been extensively studied and applied in the clinical setting. However, its clinical applicability remains controversial due to a varying degree of success and a lack of mechanistic understanding about the therapeutic effects of 3,3',5-Triiodo-L-thyronine (T-3). T-3 pre-conditioning leads to anti-apoptotic and pro-mitotic effects in liver tissue following ischemia/reperfusion injury. Therefore, we aimed to study the effects of T-3 pre-conditioning in the liver of brain-dead rats.MethodsBrain death (BD) was induced in mechanically ventilated rats by inflation of a Fogarty catheter in the epidural space. T-3 (0.1 mg/kg) or vehicle was administered intraperitoneally 2 h prior to BD induction. After 4 h of BD, serum and liver tissue were collected. RT-qPCR, routine biochemistry, and immunohistochemistry were performed.ResultsBrain-dead animals treated with T-3 had lower plasma levels of AST and ALT, reduced Bax gene expression, and less hepatic cleaved Caspase-3 activation compared to brain-dead animals treated with vehicle. Interestingly, no differences in the expression of inflammatory genes (IL-6, MCP-1, IL-1 beta) or the presence of pro-mitotic markers (Cyclin-D and Ki-67) were found in brain-dead animals treated with T-3 compared to vehicle-treated animals.ConclusionT-3 pre-conditioning leads to beneficial effects in the liver of brain-dead rats as seen by lower cellular injury and reduced apoptosis, and supports the suggested role of T-3 hormone therapy in the management of brain-dead donors.",
keywords = "POTENTIAL ORGAN DONORS, THYROID-HORMONE THERAPY, ISCHEMIA-REPERFUSION, PARTIAL-HEPATECTOMY, CLINICAL-TRIALS, KI-67 PROTEIN, MANAGEMENT, INJURY, TRANSPLANTATION, RESPONSES",
author = "{Rebolledo Acevedo}, Rolando and {Van Erp}, {Anne C.} and Ottens, {Petra J.} and Jantje Wiersema-Buist and Leuvenink, {Henri G. D.} and Pamela Romanque",
year = "2015",
month = "10",
day = "5",
doi = "10.1371/journal.pone.0138749",
language = "English",
volume = "10",
journal = "PLOS-One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "10",

}

RIS

TY - JOUR

T1 - Anti-Apoptotic Effects of 3,3 ',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats

AU - Rebolledo Acevedo, Rolando

AU - Van Erp, Anne C.

AU - Ottens, Petra J.

AU - Wiersema-Buist, Jantje

AU - Leuvenink, Henri G. D.

AU - Romanque, Pamela

PY - 2015/10/5

Y1 - 2015/10/5

N2 - BackgroundThyroid hormone treatment in brain-dead organ donors has been extensively studied and applied in the clinical setting. However, its clinical applicability remains controversial due to a varying degree of success and a lack of mechanistic understanding about the therapeutic effects of 3,3',5-Triiodo-L-thyronine (T-3). T-3 pre-conditioning leads to anti-apoptotic and pro-mitotic effects in liver tissue following ischemia/reperfusion injury. Therefore, we aimed to study the effects of T-3 pre-conditioning in the liver of brain-dead rats.MethodsBrain death (BD) was induced in mechanically ventilated rats by inflation of a Fogarty catheter in the epidural space. T-3 (0.1 mg/kg) or vehicle was administered intraperitoneally 2 h prior to BD induction. After 4 h of BD, serum and liver tissue were collected. RT-qPCR, routine biochemistry, and immunohistochemistry were performed.ResultsBrain-dead animals treated with T-3 had lower plasma levels of AST and ALT, reduced Bax gene expression, and less hepatic cleaved Caspase-3 activation compared to brain-dead animals treated with vehicle. Interestingly, no differences in the expression of inflammatory genes (IL-6, MCP-1, IL-1 beta) or the presence of pro-mitotic markers (Cyclin-D and Ki-67) were found in brain-dead animals treated with T-3 compared to vehicle-treated animals.ConclusionT-3 pre-conditioning leads to beneficial effects in the liver of brain-dead rats as seen by lower cellular injury and reduced apoptosis, and supports the suggested role of T-3 hormone therapy in the management of brain-dead donors.

AB - BackgroundThyroid hormone treatment in brain-dead organ donors has been extensively studied and applied in the clinical setting. However, its clinical applicability remains controversial due to a varying degree of success and a lack of mechanistic understanding about the therapeutic effects of 3,3',5-Triiodo-L-thyronine (T-3). T-3 pre-conditioning leads to anti-apoptotic and pro-mitotic effects in liver tissue following ischemia/reperfusion injury. Therefore, we aimed to study the effects of T-3 pre-conditioning in the liver of brain-dead rats.MethodsBrain death (BD) was induced in mechanically ventilated rats by inflation of a Fogarty catheter in the epidural space. T-3 (0.1 mg/kg) or vehicle was administered intraperitoneally 2 h prior to BD induction. After 4 h of BD, serum and liver tissue were collected. RT-qPCR, routine biochemistry, and immunohistochemistry were performed.ResultsBrain-dead animals treated with T-3 had lower plasma levels of AST and ALT, reduced Bax gene expression, and less hepatic cleaved Caspase-3 activation compared to brain-dead animals treated with vehicle. Interestingly, no differences in the expression of inflammatory genes (IL-6, MCP-1, IL-1 beta) or the presence of pro-mitotic markers (Cyclin-D and Ki-67) were found in brain-dead animals treated with T-3 compared to vehicle-treated animals.ConclusionT-3 pre-conditioning leads to beneficial effects in the liver of brain-dead rats as seen by lower cellular injury and reduced apoptosis, and supports the suggested role of T-3 hormone therapy in the management of brain-dead donors.

KW - POTENTIAL ORGAN DONORS

KW - THYROID-HORMONE THERAPY

KW - ISCHEMIA-REPERFUSION

KW - PARTIAL-HEPATECTOMY

KW - CLINICAL-TRIALS

KW - KI-67 PROTEIN

KW - MANAGEMENT

KW - INJURY

KW - TRANSPLANTATION

KW - RESPONSES

U2 - 10.1371/journal.pone.0138749

DO - 10.1371/journal.pone.0138749

M3 - Article

C2 - 26437380

VL - 10

JO - PLOS-One

JF - PLOS-One

SN - 1932-6203

IS - 10

M1 - e0138749

ER -

ID: 25419355