Publication

Angiotensin II type 2 receptor vasoactivity in internal mammary arteries of patients with coronary artery disease

de Wal, R. M. A. V., van der Harst, P., Wassmann, S., Morshuis, W. J., Nickenig, G., Buikema, H., Plokker, H. W. M., van Veldhuisen, D. J., van Gilst, W. H. & Voors, A. A., Oct-2007, In : Journal of Cardiovascular Pharmacology. 50, 4, p. 372-379 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

Background: Several animal studies suggested that the angiotensin II type 2 (AT2) receptor subtype mediates vasodilation, yet the results in human arteries are less well described and more inconsistent. Therefore, we evaluated the role of the AT2 receptor stimulation on the vasotonus of human internal mammary arteries.

Methods: Internal mammary arteries were obtained from 50 patients undergoing coronary bypass surgery. The expression of angiotensin II type I (AT1) receptor and AT2 receptor mRNA was determined by using real-time polymerase chain reaction. In addition, angiotensin II and CGP42112A concentration-response curves (concentration range: 10(-10) M to 10(-6) M) were constructed in absence or presence of candesartan (10(-5) M) and/or the AT2 receptor-antagonist PD-123319 (10-6 M) and/or the a receptor antagonist phentolamine.

Results: Both AT1 and AT2 receptor protein and mRNA were detected, and higher AT2 receptor mRNA expression levels were associated with increased contractile response to angiotensin II. Angiotensin II caused vasoconstriction up to 41.1 +/- 6.5% of the maximal response to phenylephrine, and PD123319 significantly reduced this response (28.6 +/- 9.6%, P <0.001). Candesartan completely blocked the angiotensin II-mediated response (1.4 +/- 3.1%,P <0.001 versus control), and additional blockade of the AT2 receptor with PD123319 did not change this effect (1.8 +/- 5.1%). Phentolamine (10(-5) M) caused attenuation and rightward shift of the angiotensin 11 concentration response curves. The AT2 receptor agonist CGP42112A did not induce a significant response.

Conclusion: Although AT2 receptor mRNA is present in human internal mammary arteries, AT2 receptor stimulation does not mediate vasodilation in these arteries.

Original languageEnglish
Pages (from-to)372-379
Number of pages8
JournalJournal of Cardiovascular Pharmacology
Volume50
Issue number4
Publication statusPublished - Oct-2007

    Keywords

  • angiotensin II, angiotensin II receptors, vasoconstriction, vasodilation, phenylephrine, isotonic experiments, human artery, AT(2) RECEPTOR, MESENTERIC-ARTERIES, HYPERTENSIVE-RATS, BLOOD-PRESSURE, VASODILATION, SUBTYPES, SYSTEM, VASOCONSTRICTION, IDENTIFICATION, AT2-RECEPTOR

ID: 4631820