Publication

Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis

Holmberg, M. & Nollen, E. A. A., 20-Apr-2013, Tandem Repeats in Genes, Proteins, and Disease. Hatters, D. M. & Hannan, A. J. (eds.). Humana Press, Vol. 1017. p. 193-199 7 p. (Methods in Molecular Biology; vol. 1017).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

APA

Holmberg, M., & Nollen, E. A. A. (2013). Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis. In D. M. Hatters, & A. J. Hannan (Eds.), Tandem Repeats in Genes, Proteins, and Disease (Vol. 1017, pp. 193-199). (Methods in Molecular Biology; Vol. 1017). Humana Press. https://doi.org/10.1007/978-1-62703-438-8_14

Author

Holmberg, Mats ; Nollen, Ellen A A. / Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis. Tandem Repeats in Genes, Proteins, and Disease. editor / Danny M. Hatters ; Anthony J. Hannan. Vol. 1017 Humana Press, 2013. pp. 193-199 (Methods in Molecular Biology).

Harvard

Holmberg, M & Nollen, EAA 2013, Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis. in DM Hatters & AJ Hannan (eds), Tandem Repeats in Genes, Proteins, and Disease. vol. 1017, Methods in Molecular Biology, vol. 1017, Humana Press, pp. 193-199. https://doi.org/10.1007/978-1-62703-438-8_14

Standard

Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis. / Holmberg, Mats; Nollen, Ellen A A.

Tandem Repeats in Genes, Proteins, and Disease. ed. / Danny M. Hatters; Anthony J. Hannan. Vol. 1017 Humana Press, 2013. p. 193-199 (Methods in Molecular Biology; Vol. 1017).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Vancouver

Holmberg M, Nollen EAA. Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis. In Hatters DM, Hannan AJ, editors, Tandem Repeats in Genes, Proteins, and Disease. Vol. 1017. Humana Press. 2013. p. 193-199. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-438-8_14


BibTeX

@inbook{7694b9ee1f1d436199d550c43bcdd49e,
title = "Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis",
abstract = "The accumulation of specific aggregation-prone proteins during aging is thought to be involved in several diseases, most notably Alzheimer's and Parkinson's disease as well as polyglutamine expansion disorders such as Huntington's disease. Caenorhabditis elegans disease models with transgenic expression of fluorescently tagged aggregation-prone proteins have been used to screen for genetic modifiers of aggregation. To establish the role of modifying factors in the generation of aggregation intermediates, a method has been developed using native agarose gel electrophoresis (NAGE) that enables parallel screening of aggregation patterns of fluorescently labeled aggregation-prone proteins. Together with microscopy-based genetic screens this method can be used to identify modifiers of protein aggregation and characterize their molecular function. Although described here for analyzing aggregates in C. elegans, NAGE can be adjusted for use in other model organisms as well as for cultured cells.",
keywords = "Animals, Animals, Genetically Modified, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Disease Models, Animal, Electrophoresis, Agar Gel, Heredodegenerative Disorders, Nervous System, Humans, Journal Article, Research Support, Non-U.S. Gov't",
author = "Mats Holmberg and Nollen, {Ellen A A}",
year = "2013",
month = "4",
day = "20",
doi = "10.1007/978-1-62703-438-8_14",
language = "English",
isbn = "978-1-62703-437-1",
volume = "1017",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "193--199",
editor = "Hatters, {Danny M.} and Hannan, {Anthony J.}",
booktitle = "Tandem Repeats in Genes, Proteins, and Disease",

}

RIS

TY - CHAP

T1 - Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis

AU - Holmberg, Mats

AU - Nollen, Ellen A A

PY - 2013/4/20

Y1 - 2013/4/20

N2 - The accumulation of specific aggregation-prone proteins during aging is thought to be involved in several diseases, most notably Alzheimer's and Parkinson's disease as well as polyglutamine expansion disorders such as Huntington's disease. Caenorhabditis elegans disease models with transgenic expression of fluorescently tagged aggregation-prone proteins have been used to screen for genetic modifiers of aggregation. To establish the role of modifying factors in the generation of aggregation intermediates, a method has been developed using native agarose gel electrophoresis (NAGE) that enables parallel screening of aggregation patterns of fluorescently labeled aggregation-prone proteins. Together with microscopy-based genetic screens this method can be used to identify modifiers of protein aggregation and characterize their molecular function. Although described here for analyzing aggregates in C. elegans, NAGE can be adjusted for use in other model organisms as well as for cultured cells.

AB - The accumulation of specific aggregation-prone proteins during aging is thought to be involved in several diseases, most notably Alzheimer's and Parkinson's disease as well as polyglutamine expansion disorders such as Huntington's disease. Caenorhabditis elegans disease models with transgenic expression of fluorescently tagged aggregation-prone proteins have been used to screen for genetic modifiers of aggregation. To establish the role of modifying factors in the generation of aggregation intermediates, a method has been developed using native agarose gel electrophoresis (NAGE) that enables parallel screening of aggregation patterns of fluorescently labeled aggregation-prone proteins. Together with microscopy-based genetic screens this method can be used to identify modifiers of protein aggregation and characterize their molecular function. Although described here for analyzing aggregates in C. elegans, NAGE can be adjusted for use in other model organisms as well as for cultured cells.

KW - Animals

KW - Animals, Genetically Modified

KW - Caenorhabditis elegans

KW - Caenorhabditis elegans Proteins

KW - Disease Models, Animal

KW - Electrophoresis, Agar Gel

KW - Heredodegenerative Disorders, Nervous System

KW - Humans

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/978-1-62703-438-8_14

DO - 10.1007/978-1-62703-438-8_14

M3 - Chapter

SN - 978-1-62703-437-1

VL - 1017

T3 - Methods in Molecular Biology

SP - 193

EP - 199

BT - Tandem Repeats in Genes, Proteins, and Disease

A2 - Hatters, Danny M.

A2 - Hannan, Anthony J.

PB - Humana Press

ER -

ID: 40795750