Analytical Methods in Protein-Templated Dynamic Combinatorial Chemistry

van der Vlag, R. & Hirsch, A. K. H., 22-Jun-2017, Supramolecular Medicinal Chemistry and Chemical Biology. Elsevier, Vol. 5. p. 487-509 23 p.

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Protein-templated dynamic combinatorial chemistry (DCC) has emerged as a powerful method to identify new inhibitors given that it enables the protein to select its own best binder(s) from a library of interconverting compounds. Since the first report of DCC applied to the discovery of binders for a protein, this elegant tool has been employed on a range of protein targets in early stage of medicinal-chemistry projects. A toolbox with various reversible and biocompatible reactions has become available, and the portfolio of analytical techniques is growing. Despite progress, in most cases, the libraries employed remain of moderate size due to difficulties associated with their analysis. In this article, analytical methods used in protein-templated DCC are described using examples of recent developments and reports.

Original languageEnglish
Title of host publicationSupramolecular Medicinal Chemistry and Chemical Biology
Number of pages23
ISBN (Electronic)9780128031988
ISBN (Print)9780128031995
Publication statusPublished - 22-Jun-2017


  • Analysis, Chromatography, Drug discovery, Dynamic combinatorial chemistry, Freezing (a DCL), Inhibitors, Mass spectrometry, Medicinal chemistry, NMR spectroscopy, Protein crystallography, Spectroscopy

ID: 76108493