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Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance

Wu, Z. C., Keyzer, J. D., Kusters, I. & Driessen, A. J. M., 9-Aug-2013, Membrane Biogenesis . Totowa: Humana Press, Vol. 1033. p. 157-172 16 p.

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  • Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance

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DOI

The interaction between membrane proteins and their (protein) ligands is conventionally investigated by nonequilibrium methods such as co-sedimentation or pull-down assays. Surface Plasmon Resonance can be used to monitor such binding events in real-time using isolated membranes immobilized to a surface providing insights in the kinetics of binding under equilibrium conditions. This application provides a fast, automated way to detect interacting species and to determine the kinetics and affinity (K d) of the interaction.
Original languageEnglish
Title of host publicationMembrane Biogenesis
Place of PublicationTotowa
PublisherHumana Press
Chapter11
Pages157-172
Number of pages16
Volume1033
ISBN (Electronic)978-1-62703-487-6
ISBN (Print)978-1-62703-486-9
Publication statusPublished - 9-Aug-2013

ID: 56481619