Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon ResonanceWu, Z. C., Keyzer, J. D., Kusters, I. & Driessen, A. J. M., 9-Aug-2013, Membrane Biogenesis . Totowa: Humana Press, Vol. 1033. p. 157-172 16 p. (Methods in Molecular Biology).
Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
The interaction between membrane proteins and their (protein) ligands is conventionally investigated by nonequilibrium methods such as co-sedimentation or pull-down assays. Surface Plasmon Resonance can be used to monitor such binding events in real-time using isolated membranes immobilized to a surface providing insights in the kinetics of binding under equilibrium conditions. This application provides a fast, automated way to detect interacting species and to determine the kinetics and affinity (K d) of the interaction.
|Title of host publication||Membrane Biogenesis|
|Place of Publication||Totowa|
|Number of pages||16|
|Publication status||Published - 9-Aug-2013|