Publication

Analysis of repetitive DNA in chromosomes by flow cytometry

Brind'Amour, J. & Lansdorp, P. M., Jun-2011, In : Nature Methods. 8, 6, p. 484-U71 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

We developed a flow cytometry method, chromosome flow fluorescence in situ hybridization (FISH), called CFF, to analyze repetitive DNA in chromosomes using FISH with directly labeled peptide nucleic acid (PNA) probes. We used CFF to measure the abundance of interstitial telomeric sequences in Chinese hamster chromosomes and major satellite sequences in mouse chromosomes. Using CFF we also identified parental homologs of human chromosome 18 with different amounts of repetitive DNA.

Original languageEnglish
Pages (from-to)484-U71
Number of pages5
JournalNature Methods
Volume8
Issue number6
Publication statusPublished - Jun-2011

    Keywords

  • TELOMERE LENGTH, SEQUENCE, HETEROGENEITY, HYBRIDIZATION, MICROSCOPY

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