Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS I. Sample preparation

Bischoff, R., Bronsema, K. J. & van de Merbel, N. C., 2013, In : TrAC-Trends in Analytical Chemistry. 48, p. 41-51 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard


  • Analysis of biopharmaceutical proteins in biological matrices by LC-MS-MS I. Sample

    Final publisher's version, 1.2 MB, PDF document

    Request copy


Part I of this review discusses sample-preparation aspects of quantifying biopharmaceutical proteins in complex biological matrices by LC-MS/MS with a focus on blood-derived body fluids. We conclude Part I with a short overview over options for automating the entire analytical procedure, which is indispensable for routine applications in pharmacokinetic and clinical studies. (C) 2013 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)41-51
Number of pages11
JournalTrAC-Trends in Analytical Chemistry
Publication statusPublished - 2013


  • Analytical procedure, Automation, Biological matrix, Biopharmaceuticals, Blood-derived body fluid, Clinical study, Liquid chromatography with tandem mass spectrometry (LC-MS/MS), Pharmacokinetic study, Protein, Sample preparation, TANDEM MASS-SPECTROMETRY, PERFORMANCE LIQUID-CHROMATOGRAPHY, PEPTIDE IMMUNOAFFINITY ENRICHMENT, ABSOLUTE QUANTIFICATION, MONOCLONAL-ANTIBODY, INTERNAL STANDARD, HUMAN SERUM, THERAPEUTIC PROTEINS, TRYPSIN DIGESTION, DEAMIDATION

ID: 5906034