Publication

An optimized protocol for the acute isolation of human microglia from autopsy brain samples

Olah, M., Raj, D., Brouwer, N., de Haas, A. H., Eggen, B. J. L., Den Dunnen, W. F. A., Biber, K. P. H. & Boddeke, H. W. G. M., Jan-2012, In : Glia. 60, 1, p. 96-111 16 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Olah, M., Raj, D., Brouwer, N., de Haas, A. H., Eggen, B. J. L., Den Dunnen, W. F. A., Biber, K. P. H., & Boddeke, H. W. G. M. (2012). An optimized protocol for the acute isolation of human microglia from autopsy brain samples. Glia, 60(1), 96-111. https://doi.org/10.1002/glia.21251

Author

Olah, Marta ; Raj, Divya ; Brouwer, Nieske ; de Haas, Alexander H. ; Eggen, Bart J. L. ; Den Dunnen, Wilfred F. A. ; Biber, Knut P. H. ; Boddeke, Hendrikus W. G. M. / An optimized protocol for the acute isolation of human microglia from autopsy brain samples. In: Glia. 2012 ; Vol. 60, No. 1. pp. 96-111.

Harvard

Olah, M, Raj, D, Brouwer, N, de Haas, AH, Eggen, BJL, Den Dunnen, WFA, Biber, KPH & Boddeke, HWGM 2012, 'An optimized protocol for the acute isolation of human microglia from autopsy brain samples', Glia, vol. 60, no. 1, pp. 96-111. https://doi.org/10.1002/glia.21251

Standard

An optimized protocol for the acute isolation of human microglia from autopsy brain samples. / Olah, Marta; Raj, Divya; Brouwer, Nieske; de Haas, Alexander H. ; Eggen, Bart J. L.; Den Dunnen, Wilfred F. A.; Biber, Knut P. H.; Boddeke, Hendrikus W. G. M.

In: Glia, Vol. 60, No. 1, 01.2012, p. 96-111.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Olah M, Raj D, Brouwer N, de Haas AH, Eggen BJL, Den Dunnen WFA et al. An optimized protocol for the acute isolation of human microglia from autopsy brain samples. Glia. 2012 Jan;60(1):96-111. https://doi.org/10.1002/glia.21251


BibTeX

@article{6aea1235c61d4d63b8ed4f2d90ad202e,
title = "An optimized protocol for the acute isolation of human microglia from autopsy brain samples",
abstract = "Microglia are increasingly recognized to be crucially involved in the maintenance of tissue homeostasis of the brain and spinal cord. Not surprisingly is therefore the growing scientific interest in the microglia phenotypes associated with various physiological and pathological processes of the central nervous system. Until recently the investigation of these phenotypes was hindered by the lack of an isolation protocol that (without an extended culturing period) would offer a microglia population of high purity and yield. Thus, our objective was to establish a rapid and efficient method for the isolation of human microglia from postmortem brain samples. We tested multiple elements of already existing protocols (e.g., density separation, immunomagnetic bead separation) and combined them to minimize preparation time and maximize yield and purity. The procedure presented in this article enables acute isolation of human microglia from autopsy (and biopsy) samples with a purity and yield that is suitable for downstream applications, such as protein and gene expression analysis and functional assays. Moreover, the present protocol is appropriate for the isolation of microglia from autopsy samples irrespective of the neurological state of the brain or specific brain regions and (with minor modification) could be even used for the isolation of microglia from human glioma tissue. (c) Wiley Periodicals, Inc.",
keywords = "rapid isolation, human microglia, autopsy, biopsy, CENTRAL-NERVOUS-SYSTEM, ADULT HUMAN, IN-VIVO, ANTIGEN PRESENTATION, IMMUNE-RESPONSES, CELLS, HETEROGENEITY, EXPRESSION, CULTURES, DISEASE",
author = "Marta Olah and Divya Raj and Nieske Brouwer and {de Haas}, {Alexander H.} and Eggen, {Bart J. L.} and {Den Dunnen}, {Wilfred F. A.} and Biber, {Knut P. H.} and Boddeke, {Hendrikus W. G. M.}",
year = "2012",
month = jan,
doi = "10.1002/glia.21251",
language = "English",
volume = "60",
pages = "96--111",
journal = "Glia",
issn = "0894-1491",
publisher = "Wiley",
number = "1",

}

RIS

TY - JOUR

T1 - An optimized protocol for the acute isolation of human microglia from autopsy brain samples

AU - Olah, Marta

AU - Raj, Divya

AU - Brouwer, Nieske

AU - de Haas, Alexander H.

AU - Eggen, Bart J. L.

AU - Den Dunnen, Wilfred F. A.

AU - Biber, Knut P. H.

AU - Boddeke, Hendrikus W. G. M.

PY - 2012/1

Y1 - 2012/1

N2 - Microglia are increasingly recognized to be crucially involved in the maintenance of tissue homeostasis of the brain and spinal cord. Not surprisingly is therefore the growing scientific interest in the microglia phenotypes associated with various physiological and pathological processes of the central nervous system. Until recently the investigation of these phenotypes was hindered by the lack of an isolation protocol that (without an extended culturing period) would offer a microglia population of high purity and yield. Thus, our objective was to establish a rapid and efficient method for the isolation of human microglia from postmortem brain samples. We tested multiple elements of already existing protocols (e.g., density separation, immunomagnetic bead separation) and combined them to minimize preparation time and maximize yield and purity. The procedure presented in this article enables acute isolation of human microglia from autopsy (and biopsy) samples with a purity and yield that is suitable for downstream applications, such as protein and gene expression analysis and functional assays. Moreover, the present protocol is appropriate for the isolation of microglia from autopsy samples irrespective of the neurological state of the brain or specific brain regions and (with minor modification) could be even used for the isolation of microglia from human glioma tissue. (c) Wiley Periodicals, Inc.

AB - Microglia are increasingly recognized to be crucially involved in the maintenance of tissue homeostasis of the brain and spinal cord. Not surprisingly is therefore the growing scientific interest in the microglia phenotypes associated with various physiological and pathological processes of the central nervous system. Until recently the investigation of these phenotypes was hindered by the lack of an isolation protocol that (without an extended culturing period) would offer a microglia population of high purity and yield. Thus, our objective was to establish a rapid and efficient method for the isolation of human microglia from postmortem brain samples. We tested multiple elements of already existing protocols (e.g., density separation, immunomagnetic bead separation) and combined them to minimize preparation time and maximize yield and purity. The procedure presented in this article enables acute isolation of human microglia from autopsy (and biopsy) samples with a purity and yield that is suitable for downstream applications, such as protein and gene expression analysis and functional assays. Moreover, the present protocol is appropriate for the isolation of microglia from autopsy samples irrespective of the neurological state of the brain or specific brain regions and (with minor modification) could be even used for the isolation of microglia from human glioma tissue. (c) Wiley Periodicals, Inc.

KW - rapid isolation

KW - human microglia

KW - autopsy

KW - biopsy

KW - CENTRAL-NERVOUS-SYSTEM

KW - ADULT HUMAN

KW - IN-VIVO

KW - ANTIGEN PRESENTATION

KW - IMMUNE-RESPONSES

KW - CELLS

KW - HETEROGENEITY

KW - EXPRESSION

KW - CULTURES

KW - DISEASE

U2 - 10.1002/glia.21251

DO - 10.1002/glia.21251

M3 - Article

VL - 60

SP - 96

EP - 111

JO - Glia

JF - Glia

SN - 0894-1491

IS - 1

ER -

ID: 5445961