An improved model for studies on transdermal drug absorption in-vivo in ratsVollmer, U., Muller, B. W., Wilffert, B. & Peters, T., 22-Oct-1993, In : Journal of Pharmacy and Pharmacology. 45, 4, p. 242-245 4 p.
Research output: Contribution to journal › Article › Academic › peer-review
In rats, transdermal drug absorption can be studied under physiological conditions cannulating the peripheral skin vein, draining the area of the skin which is used for drug application, and collecting the blood. This method leads to decreased blood volume, which causes a reduction in skin blood flow and limits the maximal duration of the experiment. We improved the model by replacing the collected blood with blood from donor animals, so enabling the measurement of transdermal absorption over a period of 5 h under near constant conditions of blood pressure, haematocrit and skin blood flow. The model was applied to the transdermal absorption of [3H]prazosin and [3H]scopolamine and their permeability coefficients, fluxes and lag-times were determined. The model is suitable for measurements of transdermal drug absorption under in-vivo conditions, both for comparison of absorption profiles of different drugs a of the same drug in different formulations.
|Number of pages||4|
|Journal||Journal of Pharmacy and Pharmacology|
|Publication status||Published - 22-Oct-1993|
- alcohol, dimethyl sulfoxide, prazosin, scopolamine, water, animal experiment, animal model, animal tissue, aqueous solution, arterial pressure, article, blood donor, blood pressure regulation, blood volume, cannulation, controlled study, dose time effect relation, drain, drug absorption, drug blood level, drug formulation, drug labeling, drug penetration, experimental model, hematocrit, isotope labeling, male, nonhuman, peripheral vein, permeability, rat, skin, skin blood flow, transdermal drug administration