Publication

An automated method to determine the performance of Drosophila in response to temperature changes in space and time

Soto-Padilla, A., Ruijsink, R., Span, M., van Rijn, H. & Billeter, J-C., 12-Oct-2018, In : Journal of Visualized Experiments. 2018, 140, 9 p., e58350.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Soto-Padilla, A., Ruijsink, R., Span, M., van Rijn, H., & Billeter, J-C. (2018). An automated method to determine the performance of Drosophila in response to temperature changes in space and time. Journal of Visualized Experiments, 2018(140), [ e58350]. https://doi.org/10.3791/58350

Author

Soto-Padilla, Andrea ; Ruijsink, Rick ; Span, Mark ; van Rijn, Hedderik ; Billeter, Jean-Christophe. / An automated method to determine the performance of Drosophila in response to temperature changes in space and time. In: Journal of Visualized Experiments. 2018 ; Vol. 2018, No. 140.

Harvard

Soto-Padilla, A, Ruijsink, R, Span, M, van Rijn, H & Billeter, J-C 2018, 'An automated method to determine the performance of Drosophila in response to temperature changes in space and time', Journal of Visualized Experiments, vol. 2018, no. 140, e58350. https://doi.org/10.3791/58350

Standard

An automated method to determine the performance of Drosophila in response to temperature changes in space and time. / Soto-Padilla, Andrea; Ruijsink, Rick; Span, Mark; van Rijn, Hedderik; Billeter, Jean-Christophe.

In: Journal of Visualized Experiments, Vol. 2018, No. 140, e58350, 12.10.2018.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Soto-Padilla A, Ruijsink R, Span M, van Rijn H, Billeter J-C. An automated method to determine the performance of Drosophila in response to temperature changes in space and time. Journal of Visualized Experiments. 2018 Oct 12;2018(140). e58350. https://doi.org/10.3791/58350


BibTeX

@article{10bfb4b1c164455abdd5d7d053f80fc5,
title = "An automated method to determine the performance of Drosophila in response to temperature changes in space and time",
abstract = "Temperature is a ubiquitous environmental factor that affects how species distribute and behave. Different species of Drosophila fruit flies have specific responses to changing temperatures according to their physiological tolerance and adaptability. Drosophila flies also possess a temperature sensing system that has become fundamental to understanding the neural basis of temperature processing in ectotherms. We present here a temperature-controlled arena that permits fast and precise temperature changes with temporal and spatial control to explore the response of individual flies to changing temperatures. Individual flies are placed in the arena and exposed to pre-programmed temperature challenges, such as uniform gradual increases in temperature to determine reaction norms or spatially distributed temperatures at the same time to determine preferences. Individuals are automatically tracked, allowing the quantification of speed or location preference. This method can be used to rapidly quantify the response over a large range of temperatures to determine temperature performance curves in Drosophila or other insects of similar size. In addition, it can be used for genetic studies to quantify temperature preferences and reactions of mutants or wild-type flies. This method can help uncover the basis of thermal speciation and adaptation, as well as the neural mechanisms behind temperature processing.",
author = "Andrea Soto-Padilla and Rick Ruijsink and Mark Span and {van Rijn}, Hedderik and Jean-Christophe Billeter",
year = "2018",
month = "10",
day = "12",
doi = "10.3791/58350",
language = "English",
volume = "2018",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "JOURNAL OF VISUALIZED EXPERIMENTS",
number = "140",

}

RIS

TY - JOUR

T1 - An automated method to determine the performance of Drosophila in response to temperature changes in space and time

AU - Soto-Padilla, Andrea

AU - Ruijsink, Rick

AU - Span, Mark

AU - van Rijn, Hedderik

AU - Billeter, Jean-Christophe

PY - 2018/10/12

Y1 - 2018/10/12

N2 - Temperature is a ubiquitous environmental factor that affects how species distribute and behave. Different species of Drosophila fruit flies have specific responses to changing temperatures according to their physiological tolerance and adaptability. Drosophila flies also possess a temperature sensing system that has become fundamental to understanding the neural basis of temperature processing in ectotherms. We present here a temperature-controlled arena that permits fast and precise temperature changes with temporal and spatial control to explore the response of individual flies to changing temperatures. Individual flies are placed in the arena and exposed to pre-programmed temperature challenges, such as uniform gradual increases in temperature to determine reaction norms or spatially distributed temperatures at the same time to determine preferences. Individuals are automatically tracked, allowing the quantification of speed or location preference. This method can be used to rapidly quantify the response over a large range of temperatures to determine temperature performance curves in Drosophila or other insects of similar size. In addition, it can be used for genetic studies to quantify temperature preferences and reactions of mutants or wild-type flies. This method can help uncover the basis of thermal speciation and adaptation, as well as the neural mechanisms behind temperature processing.

AB - Temperature is a ubiquitous environmental factor that affects how species distribute and behave. Different species of Drosophila fruit flies have specific responses to changing temperatures according to their physiological tolerance and adaptability. Drosophila flies also possess a temperature sensing system that has become fundamental to understanding the neural basis of temperature processing in ectotherms. We present here a temperature-controlled arena that permits fast and precise temperature changes with temporal and spatial control to explore the response of individual flies to changing temperatures. Individual flies are placed in the arena and exposed to pre-programmed temperature challenges, such as uniform gradual increases in temperature to determine reaction norms or spatially distributed temperatures at the same time to determine preferences. Individuals are automatically tracked, allowing the quantification of speed or location preference. This method can be used to rapidly quantify the response over a large range of temperatures to determine temperature performance curves in Drosophila or other insects of similar size. In addition, it can be used for genetic studies to quantify temperature preferences and reactions of mutants or wild-type flies. This method can help uncover the basis of thermal speciation and adaptation, as well as the neural mechanisms behind temperature processing.

U2 - 10.3791/58350

DO - 10.3791/58350

M3 - Article

VL - 2018

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 140

M1 - e58350

ER -

ID: 66996965