Publication

Amrinone suppresses the synthesis of tumor necrosis factor-alpha in human mononuclear cells

Endres, S., Sinha, B. & Fülle, H. J., May-1994, In : Shock. 1, 5, p. 377-380 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

Tumor necrosis factor-alpha (TNF) exerts a wide spectrum of biological activities and contributes to the pathophysiology of septic shock. Elevated circulating levels of TNF have also been reported in patients with severe chronic heart failure. We studied the effect of amrinone, a class III cyclic nucleotide phosphodiesterase inhibitor used in the treatment of acute heart failure, on the synthesis of TNF in vitro. Peripheral blood mononuclear cells from healthy volunteers or cells of a permanent monoblast cell line were stimulated for 20 h with bacterial lipopolysaccharide and different doses of amrinone. TNF production is suppressed in a dose-dependent manner to a minimum of 9% of controls with 1000 microM of amrinone, reaching half-maximal inhibition at 80 microM amrinone. This effect appears to be mediated via cAMP, which accumulated nearly twofold in the presence of amrinone. Suppression of TNF synthesis by therapeutically administered phosphodiesterase inhibitors such as amrinone may contribute to their beneficial effect in the treatment of heart failure.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalShock
Volume1
Issue number5
Publication statusPublished - May-1994

    Keywords

  • Amrinone, Cell Line, Cyclic AMP, Humans, Lipopolysaccharides, Monocytes, Radioimmunoassay, Tumor Necrosis Factor-alpha

ID: 13978496