Publication

ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency

Ord, R. L., Tami, A. & Sutherland, C. J., 2008, In : PLoS ONE. 3, 10, 10 p., e3366.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Ord, R. L., Tami, A., & Sutherland, C. J. (2008). ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency. PLoS ONE, 3(10), [e3366]. https://doi.org/10.1371/journal.pone.0003366

Author

Ord, Rosalynn L ; Tami, Adriana ; Sutherland, Colin J. / ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency. In: PLoS ONE. 2008 ; Vol. 3, No. 10.

Harvard

Ord, RL, Tami, A & Sutherland, CJ 2008, 'ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency', PLoS ONE, vol. 3, no. 10, e3366. https://doi.org/10.1371/journal.pone.0003366

Standard

ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency. / Ord, Rosalynn L; Tami, Adriana; Sutherland, Colin J.

In: PLoS ONE, Vol. 3, No. 10, e3366, 2008.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Ord RL, Tami A, Sutherland CJ. ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency. PLoS ONE. 2008;3(10). e3366. https://doi.org/10.1371/journal.pone.0003366


BibTeX

@article{abd0a9fe765c4a839bfecbe407d4c543,
title = "ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency",
abstract = "BACKGROUND: We present the first population genetic analysis of homologous loci from two sympatric human malaria parasite populations sharing the same human hosts, using full-length sequences of ama1 genes from Plasmodium vivax and P. falciparum collected in the Venezuelan Amazon.METHODOLOGY/PRINCIPAL FINDINGS: Significant differences between the two species were found in genetic diversity at the ama1 locus, with 18 distinct haplotypes identified among the 73 Pvama1 sequences obtained, compared to 6 unique haplotypes from 30 Pfama1 sequences, giving overall diversity estimates of h = 0.9091, and h = 0.538 respectively. Levels of recombination were also found to differ between the species, with P. falciparum exhibiting very little recombination across the 1.77 kb sequence. In contrast, analysis of patterns of nucleotide substitutions provided evidence that polymorphisms in the ama1 gene of both species are maintained by balancing selection, particularly in domain I. The two distinct population structures observed are unlikely to result from different selective forces acting upon the two species, which share both human and mosquito hosts in this setting. Rather, the highly structured P. falciparum population appears to be the result of a population bottleneck, while the much less structured P. vivax population is likely to be derived from an ancient pool of diversity, as reflected in a larger estimate of effective population size for this species. Greatly reduced mosquito transmission in 1997, due to low rainfall prior to the second survey, was associated with far fewer P. falciparum infections, but an increase in P. vivax infections, probably due to hypnozoite activation.CONCLUSIONS/SIGNIFICANCE: The relevance of these findings to putative competitive interactions between these two important human pathogen species is discussed. These results highlight the need for future control interventions to employ strategies targeting each of the parasite species present in endemic areas.",
keywords = "Animals, Antigens, Protozoan/genetics, Base Sequence, Gene Frequency, Genetic Variation, Genetics, Population, Haplotypes, Humans, Linkage Disequilibrium, Malaria/genetics, Membrane Proteins/genetics, Plasmodium falciparum/genetics, Plasmodium vivax/genetics, Protozoan Proteins/genetics, Recombination, Genetic, Selection, Genetic, Venezuela",
author = "Ord, {Rosalynn L} and Adriana Tami and Sutherland, {Colin J}",
year = "2008",
doi = "10.1371/journal.pone.0003366",
language = "English",
volume = "3",
journal = "PLOS-One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "10",

}

RIS

TY - JOUR

T1 - ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency

AU - Ord, Rosalynn L

AU - Tami, Adriana

AU - Sutherland, Colin J

PY - 2008

Y1 - 2008

N2 - BACKGROUND: We present the first population genetic analysis of homologous loci from two sympatric human malaria parasite populations sharing the same human hosts, using full-length sequences of ama1 genes from Plasmodium vivax and P. falciparum collected in the Venezuelan Amazon.METHODOLOGY/PRINCIPAL FINDINGS: Significant differences between the two species were found in genetic diversity at the ama1 locus, with 18 distinct haplotypes identified among the 73 Pvama1 sequences obtained, compared to 6 unique haplotypes from 30 Pfama1 sequences, giving overall diversity estimates of h = 0.9091, and h = 0.538 respectively. Levels of recombination were also found to differ between the species, with P. falciparum exhibiting very little recombination across the 1.77 kb sequence. In contrast, analysis of patterns of nucleotide substitutions provided evidence that polymorphisms in the ama1 gene of both species are maintained by balancing selection, particularly in domain I. The two distinct population structures observed are unlikely to result from different selective forces acting upon the two species, which share both human and mosquito hosts in this setting. Rather, the highly structured P. falciparum population appears to be the result of a population bottleneck, while the much less structured P. vivax population is likely to be derived from an ancient pool of diversity, as reflected in a larger estimate of effective population size for this species. Greatly reduced mosquito transmission in 1997, due to low rainfall prior to the second survey, was associated with far fewer P. falciparum infections, but an increase in P. vivax infections, probably due to hypnozoite activation.CONCLUSIONS/SIGNIFICANCE: The relevance of these findings to putative competitive interactions between these two important human pathogen species is discussed. These results highlight the need for future control interventions to employ strategies targeting each of the parasite species present in endemic areas.

AB - BACKGROUND: We present the first population genetic analysis of homologous loci from two sympatric human malaria parasite populations sharing the same human hosts, using full-length sequences of ama1 genes from Plasmodium vivax and P. falciparum collected in the Venezuelan Amazon.METHODOLOGY/PRINCIPAL FINDINGS: Significant differences between the two species were found in genetic diversity at the ama1 locus, with 18 distinct haplotypes identified among the 73 Pvama1 sequences obtained, compared to 6 unique haplotypes from 30 Pfama1 sequences, giving overall diversity estimates of h = 0.9091, and h = 0.538 respectively. Levels of recombination were also found to differ between the species, with P. falciparum exhibiting very little recombination across the 1.77 kb sequence. In contrast, analysis of patterns of nucleotide substitutions provided evidence that polymorphisms in the ama1 gene of both species are maintained by balancing selection, particularly in domain I. The two distinct population structures observed are unlikely to result from different selective forces acting upon the two species, which share both human and mosquito hosts in this setting. Rather, the highly structured P. falciparum population appears to be the result of a population bottleneck, while the much less structured P. vivax population is likely to be derived from an ancient pool of diversity, as reflected in a larger estimate of effective population size for this species. Greatly reduced mosquito transmission in 1997, due to low rainfall prior to the second survey, was associated with far fewer P. falciparum infections, but an increase in P. vivax infections, probably due to hypnozoite activation.CONCLUSIONS/SIGNIFICANCE: The relevance of these findings to putative competitive interactions between these two important human pathogen species is discussed. These results highlight the need for future control interventions to employ strategies targeting each of the parasite species present in endemic areas.

KW - Animals

KW - Antigens, Protozoan/genetics

KW - Base Sequence

KW - Gene Frequency

KW - Genetic Variation

KW - Genetics, Population

KW - Haplotypes

KW - Humans

KW - Linkage Disequilibrium

KW - Malaria/genetics

KW - Membrane Proteins/genetics

KW - Plasmodium falciparum/genetics

KW - Plasmodium vivax/genetics

KW - Protozoan Proteins/genetics

KW - Recombination, Genetic

KW - Selection, Genetic

KW - Venezuela

U2 - 10.1371/journal.pone.0003366

DO - 10.1371/journal.pone.0003366

M3 - Article

C2 - 18846221

VL - 3

JO - PLOS-One

JF - PLOS-One

SN - 1932-6203

IS - 10

M1 - e3366

ER -

ID: 74394013