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Altered immune response of immature dendritic cells upon dengue virus infection in the presence of specific antibodies

Torres, S., Flipse, J., Upasani, V. C., van der Ende-Metselaar, H., Urcuqui-Inchima, S., Smit, J. M. & Rodenhuis-Zybert, I. A., Jul-2016, In : The Journal of general virology. 97, 7, p. 1584-1591 8 p.

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  • Altered immune response of immature dendritic cells upon dengue virus

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DOI

Dengue virus (DENV) replication is known to prevent maturation of infected dendritic cells (DCs) thereby impeding the development of adequate immunity. During secondary DENV infection, dengue-specific antibodies can suppress DENV replication in immature DCs (immDCs), however how dengue-antibody complexes (DENV-IC) influence the phenotype of DCs remains elusive. Here, we evaluated the maturation state and cytokine profile of immDCs exposed to DENV-ICs. Indeed, DENV infection of immDCs in the absence of antibodies was hallmarked by blunted upregulation of CD83, CD86 and the major histocompatibility complex molecule HLA-DR. In contrast, DENV infection in the presence of neutralizing antibodies triggered full DC maturation and induced a balanced inflammatory cytokine response. Moreover, DENV infection under non-neutralizing conditions prompted upregulation of CD83 and CD86 but not HLA-DR, and triggered production of pro inflammatory cytokines. The effect of DENV-IC was found to be dependent on the engagement of FcyRIla. Altogether, our data show that the presence of DENV-IC alters the phenotype and cytokine profile of DCs.

Original languageEnglish
Pages (from-to)1584-1591
Number of pages8
JournalThe Journal of general virology
Volume97
Issue number7
Publication statusPublished - Jul-2016

    Keywords

  • FC-GAMMA-RIIA, DEPENDENT ENHANCEMENT, HEMORRHAGIC-FEVER, ANTIGEN PRESENTATION, CYTOKINE PRODUCTION, SEMI-MATURE, INTERFERON, MATURATION, DISEASE, COMPLEX

ID: 31928712