Publication

Alpha-Helical Fragaceatoxin C Nanopore Engineered for Double-Stranded and Single-Stranded Nucleic Acid Analysis

Wloka, C., Mutter, N. L., Soskine, M. & Maglia, G., 26-Sep-2016, In : Angewandte Chemie - International Edition. 55, 40, p. 12494-12498 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • Alpha-Helical Fragaceatoxin C Nanopore Engineered for Double-Stranded and Single-Stranded Nucleic Acid Analysis

    Final publisher's version, 2 MB, PDF document

    Request copy

DOI

Nanopores are used in single-molecule DNA analysis and sequencing. Herein, we show that Fragaceatoxin C (FraC), an α-helical pore-forming toxin from an actinoporin protein family, can be reconstituted in sphingomyelin-free standard planar lipid bilayers. We engineered FraC for DNA analysis and show that the funnel-shaped geometry allows tight wrapping around single-stranded DNA (ssDNA), resolving between homopolymeric C, T, and A polynucleotide stretches. Remarkably, despite the 1.2 nm internal constriction of FraC, double-stranded DNA (dsDNA) can translocate through the nanopore at high applied potentials, presumably through the deformation of the α-helical transmembrane region of the pore. Therefore, FraC nanopores might be used in DNA sequencing and dsDNA analysis.

Original languageEnglish
Pages (from-to)12494-12498
Number of pages5
JournalAngewandte Chemie - International Edition
Volume55
Issue number40
Publication statusPublished - 26-Sep-2016

    Keywords

  • alpha-helical fragaceatoxin, nanopores, nucleic acids, chemical biology

ID: 35297033