Publication

Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

Winczura, K., Domanski, M. & LaCava, J., 26-Nov-2019, Humana, New York, NY. 35 p. (Methods in Molecular Biology)

Research output: Book/ReportProtocolProfessional

APA

Winczura, K., Domanski, M., & LaCava, J. (2019). Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes. (Methods in Molecular Biology). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9822-7_15

Author

Winczura, Kinga ; Domanski, Michal ; LaCava, John. / Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes. Humana, New York, NY, 2019. 35 p. (Methods in Molecular Biology).

Harvard

Winczura, K, Domanski, M & LaCava, J 2019, Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes. Methods in Molecular Biology, vol. 2062, Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9822-7_15

Standard

Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes. / Winczura, Kinga; Domanski, Michal; LaCava, John.

Humana, New York, NY, 2019. 35 p. (Methods in Molecular Biology).

Research output: Book/ReportProtocolProfessional

Vancouver

Winczura K, Domanski M, LaCava J. Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes. Humana, New York, NY, 2019. 35 p. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9822-7_15


BibTeX

@book{65bcc743f7764a269993ad4cc7e2910d,
title = "Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes",
abstract = "In humans, the RNA exosome consists of an enzymatically inactive nine-subunit core, with ribonucleolytic activity contributed by additional components. Several cofactor complexes also interact with the exosome-these enable the recruitment of, and specify the activity upon, diverse substrates. Affinity capture coupled with mass spectrometry has proven to be an effective means to identify the compositions of RNA exosomes and their cofactor complexes: here, we describe a general experimental strategy for proteomic characterization of macromolecular complexes, applied to the exosome and an affiliated adapter protein, ZC3H18.",
author = "Kinga Winczura and Michal Domanski and John LaCava",
year = "2019",
month = nov,
day = "26",
doi = "10.1007/978-1-4939-9822-7_15",
language = "English",
isbn = "978-1-4939-9821-0",
volume = "2062",
series = "Methods in Molecular Biology",
publisher = "Humana, New York, NY",

}

RIS

TY - BOOK

T1 - Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

AU - Winczura, Kinga

AU - Domanski, Michal

AU - LaCava, John

PY - 2019/11/26

Y1 - 2019/11/26

N2 - In humans, the RNA exosome consists of an enzymatically inactive nine-subunit core, with ribonucleolytic activity contributed by additional components. Several cofactor complexes also interact with the exosome-these enable the recruitment of, and specify the activity upon, diverse substrates. Affinity capture coupled with mass spectrometry has proven to be an effective means to identify the compositions of RNA exosomes and their cofactor complexes: here, we describe a general experimental strategy for proteomic characterization of macromolecular complexes, applied to the exosome and an affiliated adapter protein, ZC3H18.

AB - In humans, the RNA exosome consists of an enzymatically inactive nine-subunit core, with ribonucleolytic activity contributed by additional components. Several cofactor complexes also interact with the exosome-these enable the recruitment of, and specify the activity upon, diverse substrates. Affinity capture coupled with mass spectrometry has proven to be an effective means to identify the compositions of RNA exosomes and their cofactor complexes: here, we describe a general experimental strategy for proteomic characterization of macromolecular complexes, applied to the exosome and an affiliated adapter protein, ZC3H18.

U2 - 10.1007/978-1-4939-9822-7_15

DO - 10.1007/978-1-4939-9822-7_15

M3 - Protocol

C2 - 31768983

SN - 978-1-4939-9821-0

VL - 2062

T3 - Methods in Molecular Biology

BT - Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

PB - Humana, New York, NY

ER -

ID: 117141453