Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

Winczura, K., Domanski, M. & LaCava, J., 26-Nov-2019, Humana, New York, NY. 35 p. (Methods in Molecular Biology)

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In humans, the RNA exosome consists of an enzymatically inactive nine-subunit core, with ribonucleolytic activity contributed by additional components. Several cofactor complexes also interact with the exosome-these enable the recruitment of, and specify the activity upon, diverse substrates. Affinity capture coupled with mass spectrometry has proven to be an effective means to identify the compositions of RNA exosomes and their cofactor complexes: here, we describe a general experimental strategy for proteomic characterization of macromolecular complexes, applied to the exosome and an affiliated adapter protein, ZC3H18.

Original languageEnglish
PublisherHumana, New York, NY
Number of pages35
ISBN (Electronic)978-1-4939-9822-7
ISBN (Print)978-1-4939-9821-0
Publication statusE-pub ahead of print - 26-Nov-2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

ID: 117141453