Publication

Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions

Teekamp, N., Tian, Y., Visser, J. C., Olinga, P., Frijlink, H. W., Woerdenbag, H. J. & Hinrichs, W. L. J., 15-Nov-2017, In : Carbohydrate Polymers. 176, p. 374-380 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Teekamp, N., Tian, Y., Visser, J. C., Olinga, P., Frijlink, H. W., Woerdenbag, H. J., & Hinrichs, W. L. J. (2017). Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions. Carbohydrate Polymers, 176, 374-380. https://doi.org/10.1016/j.carbpol.2017.08.084

Author

Teekamp, Naomi ; Tian, Yu ; Visser, J. Carolina ; Olinga, Peter ; Frijlink, Henderik W. ; Woerdenbag, Herman J. ; Hinrichs, Wouter L. J. / Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions. In: Carbohydrate Polymers. 2017 ; Vol. 176. pp. 374-380.

Harvard

Teekamp, N, Tian, Y, Visser, JC, Olinga, P, Frijlink, HW, Woerdenbag, HJ & Hinrichs, WLJ 2017, 'Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions', Carbohydrate Polymers, vol. 176, pp. 374-380. https://doi.org/10.1016/j.carbpol.2017.08.084

Standard

Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions. / Teekamp, Naomi; Tian, Yu; Visser, J. Carolina; Olinga, Peter; Frijlink, Henderik W.; Woerdenbag, Herman J.; Hinrichs, Wouter L. J.

In: Carbohydrate Polymers, Vol. 176, 15.11.2017, p. 374-380.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Teekamp N, Tian Y, Visser JC, Olinga P, Frijlink HW, Woerdenbag HJ et al. Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions. Carbohydrate Polymers. 2017 Nov 15;176:374-380. https://doi.org/10.1016/j.carbpol.2017.08.084


BibTeX

@article{c2e2ff9058b944dca8ae37b92e18bb7c,
title = "Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions",
abstract = "Incorporation of therapeutic proteins in a matrix of sugar glass is known to enhance protein stability, yet protection is often lost when exposed to high relative humidity (RH). We hypothesized that especially in these conditions the use of binary glasses of a polysaccharide and disaccharide might yield advantages for protein stability. Therefore, different amounts of the polysaccharide pullulan were introduced in freeze-dried trehalose glasses. In these homogeneous blends, the presence of pullulan above 50 weight {\%} prevented crystallization of trehalose when exposed to high RH. Storage stability testing up to 4 weeks of the model protein β-galactosidase incorporated in pullulan/trehalose blends showed superior behavior of pure trehalose at 30°C/0{\%} RH, while pullulan/trehalose blends yielded the best stability at 30°C/56{\%} RH. In conclusion, binary glasses of pullulan and trehalose may provide excellent stability of proteins under storage conditions that may occur in practice, namely high temperature and high RH.",
keywords = "pullulan, trehalose, protein stabilization, sugar glass, dynamic vapor sorption, differential scanning calorimetry, STORAGE STABILITY, SOLID-STATE, LYOPHILIZATE COLLAPSE, THERAPEUTIC PROTEINS, STABILIZATION, SUGAR, DELIVERY, SYSTEMS, MATRIX, EXOPOLYSACCHARIDE",
author = "Naomi Teekamp and Yu Tian and Visser, {J. Carolina} and Peter Olinga and Frijlink, {Henderik W.} and Woerdenbag, {Herman J.} and Hinrichs, {Wouter L. J.}",
note = "Copyright {\circledC} 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.",
year = "2017",
month = "11",
day = "15",
doi = "10.1016/j.carbpol.2017.08.084",
language = "English",
volume = "176",
pages = "374--380",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "ELSEVIER SCI LTD",

}

RIS

TY - JOUR

T1 - Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions

AU - Teekamp, Naomi

AU - Tian, Yu

AU - Visser, J. Carolina

AU - Olinga, Peter

AU - Frijlink, Henderik W.

AU - Woerdenbag, Herman J.

AU - Hinrichs, Wouter L. J.

N1 - Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

PY - 2017/11/15

Y1 - 2017/11/15

N2 - Incorporation of therapeutic proteins in a matrix of sugar glass is known to enhance protein stability, yet protection is often lost when exposed to high relative humidity (RH). We hypothesized that especially in these conditions the use of binary glasses of a polysaccharide and disaccharide might yield advantages for protein stability. Therefore, different amounts of the polysaccharide pullulan were introduced in freeze-dried trehalose glasses. In these homogeneous blends, the presence of pullulan above 50 weight % prevented crystallization of trehalose when exposed to high RH. Storage stability testing up to 4 weeks of the model protein β-galactosidase incorporated in pullulan/trehalose blends showed superior behavior of pure trehalose at 30°C/0% RH, while pullulan/trehalose blends yielded the best stability at 30°C/56% RH. In conclusion, binary glasses of pullulan and trehalose may provide excellent stability of proteins under storage conditions that may occur in practice, namely high temperature and high RH.

AB - Incorporation of therapeutic proteins in a matrix of sugar glass is known to enhance protein stability, yet protection is often lost when exposed to high relative humidity (RH). We hypothesized that especially in these conditions the use of binary glasses of a polysaccharide and disaccharide might yield advantages for protein stability. Therefore, different amounts of the polysaccharide pullulan were introduced in freeze-dried trehalose glasses. In these homogeneous blends, the presence of pullulan above 50 weight % prevented crystallization of trehalose when exposed to high RH. Storage stability testing up to 4 weeks of the model protein β-galactosidase incorporated in pullulan/trehalose blends showed superior behavior of pure trehalose at 30°C/0% RH, while pullulan/trehalose blends yielded the best stability at 30°C/56% RH. In conclusion, binary glasses of pullulan and trehalose may provide excellent stability of proteins under storage conditions that may occur in practice, namely high temperature and high RH.

KW - pullulan

KW - trehalose

KW - protein stabilization

KW - sugar glass

KW - dynamic vapor sorption

KW - differential scanning calorimetry

KW - STORAGE STABILITY

KW - SOLID-STATE

KW - LYOPHILIZATE COLLAPSE

KW - THERAPEUTIC PROTEINS

KW - STABILIZATION

KW - SUGAR

KW - DELIVERY

KW - SYSTEMS

KW - MATRIX

KW - EXOPOLYSACCHARIDE

U2 - 10.1016/j.carbpol.2017.08.084

DO - 10.1016/j.carbpol.2017.08.084

M3 - Article

VL - 176

SP - 374

EP - 380

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -

ID: 48243821