Publication

Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects

Chi Hai Vu, Kolata, J., Stentzel, S., Beyer, A., Salazar, M. G., Steil, L., Pane-Farre, J., Ruehmling, V., Engelmann, S., Goetz, F., van Dijl, J. M., Hecker, M., Maeder, U., Schmidt, F., Voelker, U. & Broeker, B. M., Oct-2016, In : Proteomics. 16, 20, p. 2667-2677 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Chi Hai Vu, Kolata, J., Stentzel, S., Beyer, A., Salazar, M. G., Steil, L., ... Broeker, B. M. (2016). Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects. Proteomics, 16(20), 2667-2677. https://doi.org/10.1002/pmic.201600151

Author

Chi Hai Vu ; Kolata, Julia ; Stentzel, Sebastian ; Beyer, Anica ; Salazar, Manuela Gesell ; Steil, Leif ; Pane-Farre, Jan ; Ruehmling, Vanessa ; Engelmann, Susanne ; Goetz, Friedrich ; van Dijl, Jan Maarten ; Hecker, Michael ; Maeder, Ulrike ; Schmidt, Frank ; Voelker, Uwe ; Broeker, Barbara M. / Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects. In: Proteomics. 2016 ; Vol. 16, No. 20. pp. 2667-2677.

Harvard

Chi Hai Vu, Kolata, J, Stentzel, S, Beyer, A, Salazar, MG, Steil, L, Pane-Farre, J, Ruehmling, V, Engelmann, S, Goetz, F, van Dijl, JM, Hecker, M, Maeder, U, Schmidt, F, Voelker, U & Broeker, BM 2016, 'Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects', Proteomics, vol. 16, no. 20, pp. 2667-2677. https://doi.org/10.1002/pmic.201600151

Standard

Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects. / Chi Hai Vu; Kolata, Julia; Stentzel, Sebastian; Beyer, Anica; Salazar, Manuela Gesell; Steil, Leif; Pane-Farre, Jan; Ruehmling, Vanessa; Engelmann, Susanne; Goetz, Friedrich; van Dijl, Jan Maarten; Hecker, Michael; Maeder, Ulrike; Schmidt, Frank; Voelker, Uwe; Broeker, Barbara M.

In: Proteomics, Vol. 16, No. 20, 10.2016, p. 2667-2677.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Chi Hai Vu, Kolata J, Stentzel S, Beyer A, Salazar MG, Steil L et al. Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects. Proteomics. 2016 Oct;16(20):2667-2677. https://doi.org/10.1002/pmic.201600151


BibTeX

@article{f6bd3187c7b141b6a0f4bb3968995daa,
title = "Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects",
abstract = "Staphylococcus aureus is a frequent commensal but also a dangerous pathogen, causing many forms of infection ranging from mild to life-threatening conditions. Among its virulence factors are lipoproteins, which are anchored in the bacterial cell membrane. Lipoproteins perform various functions in colonization, immune evasion, and immunomodulation. These proteins are potent activators of innate immune receptors termed Toll-like receptors 2 and 6. This study addressed the specific B-cell and T-cell responses directed to lipoproteins in human S. aureus carriers and non-carriers. 2D immune proteomics and ELISA approaches revealed that titers of antibodies (IgG) binding to S. aureus lipoproteins were very low. Proliferation assays and cytokine profiling data showed only subtle responses of T cells; some lipoproteins did not elicit proliferation. Hence, the robust activation of the innate immune system by S. aureus lipoproteins does not translate into a strong adaptive immune response. Reasons for this may include inaccessibility of lipoproteins for B cells as well as ineffective processing and presentation of the antigens to T cells.",
keywords = "Antibody, Human, Lipoprotein, Microbiology, S. aureus, T cell, ANTIBODY-RESPONSE, STAPHYLOFERRIN B, ABC-TRANSPORTER, NASAL CARRIAGE, RECEPTOR 2, CELL-WALL, IN-VIVO, VACCINE, PROTEOME, BACTERIA",
author = "{Chi Hai Vu} and Julia Kolata and Sebastian Stentzel and Anica Beyer and Salazar, {Manuela Gesell} and Leif Steil and Jan Pane-Farre and Vanessa Ruehmling and Susanne Engelmann and Friedrich Goetz and {van Dijl}, {Jan Maarten} and Michael Hecker and Ulrike Maeder and Frank Schmidt and Uwe Voelker and Broeker, {Barbara M.}",
note = "This article is protected by copyright. All rights reserved.",
year = "2016",
month = "10",
doi = "10.1002/pmic.201600151",
language = "English",
volume = "16",
pages = "2667--2677",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley",
number = "20",

}

RIS

TY - JOUR

T1 - Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects

AU - Chi Hai Vu, null

AU - Kolata, Julia

AU - Stentzel, Sebastian

AU - Beyer, Anica

AU - Salazar, Manuela Gesell

AU - Steil, Leif

AU - Pane-Farre, Jan

AU - Ruehmling, Vanessa

AU - Engelmann, Susanne

AU - Goetz, Friedrich

AU - van Dijl, Jan Maarten

AU - Hecker, Michael

AU - Maeder, Ulrike

AU - Schmidt, Frank

AU - Voelker, Uwe

AU - Broeker, Barbara M.

N1 - This article is protected by copyright. All rights reserved.

PY - 2016/10

Y1 - 2016/10

N2 - Staphylococcus aureus is a frequent commensal but also a dangerous pathogen, causing many forms of infection ranging from mild to life-threatening conditions. Among its virulence factors are lipoproteins, which are anchored in the bacterial cell membrane. Lipoproteins perform various functions in colonization, immune evasion, and immunomodulation. These proteins are potent activators of innate immune receptors termed Toll-like receptors 2 and 6. This study addressed the specific B-cell and T-cell responses directed to lipoproteins in human S. aureus carriers and non-carriers. 2D immune proteomics and ELISA approaches revealed that titers of antibodies (IgG) binding to S. aureus lipoproteins were very low. Proliferation assays and cytokine profiling data showed only subtle responses of T cells; some lipoproteins did not elicit proliferation. Hence, the robust activation of the innate immune system by S. aureus lipoproteins does not translate into a strong adaptive immune response. Reasons for this may include inaccessibility of lipoproteins for B cells as well as ineffective processing and presentation of the antigens to T cells.

AB - Staphylococcus aureus is a frequent commensal but also a dangerous pathogen, causing many forms of infection ranging from mild to life-threatening conditions. Among its virulence factors are lipoproteins, which are anchored in the bacterial cell membrane. Lipoproteins perform various functions in colonization, immune evasion, and immunomodulation. These proteins are potent activators of innate immune receptors termed Toll-like receptors 2 and 6. This study addressed the specific B-cell and T-cell responses directed to lipoproteins in human S. aureus carriers and non-carriers. 2D immune proteomics and ELISA approaches revealed that titers of antibodies (IgG) binding to S. aureus lipoproteins were very low. Proliferation assays and cytokine profiling data showed only subtle responses of T cells; some lipoproteins did not elicit proliferation. Hence, the robust activation of the innate immune system by S. aureus lipoproteins does not translate into a strong adaptive immune response. Reasons for this may include inaccessibility of lipoproteins for B cells as well as ineffective processing and presentation of the antigens to T cells.

KW - Antibody

KW - Human

KW - Lipoprotein

KW - Microbiology

KW - S. aureus

KW - T cell

KW - ANTIBODY-RESPONSE

KW - STAPHYLOFERRIN B

KW - ABC-TRANSPORTER

KW - NASAL CARRIAGE

KW - RECEPTOR 2

KW - CELL-WALL

KW - IN-VIVO

KW - VACCINE

KW - PROTEOME

KW - BACTERIA

U2 - 10.1002/pmic.201600151

DO - 10.1002/pmic.201600151

M3 - Article

VL - 16

SP - 2667

EP - 2677

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 20

ER -

ID: 33546434