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Acute seizure-suppressing effect of vagus nerve stimulation in the amygdala kindled rat

Rijkers, K., Aalbers, M., Hoogland, G., van Winden, L., Vles, J., Steinbusch, H. & Majoie, M., 10-Mar-2010, In : Brain Research. 1319, p. 155-163 9 p.

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  • Acute seizure-suppressing effect of vagus nerve stimulation in the amygdala kindled rat

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DOI

  • Kim Rijkers
  • Marlien Aalbers
  • Govert Hoogland
  • Larissa van Winden
  • Johannes Vles
  • Harry Steinbusch
  • Marian Majoie

Purpose: Vagus nerve stimulation (VNS) is a moderately effective anti-epileptic treatment. Clinically relevant animal models that are suitable to study the mechanism of action of VNS are not available. The aim of the current study was to develop a clinically relevant animal model for VNS-treated epilepsy that can be used to study the mechanism of action of VNS. Methods: The anticonvulsive effect of VNS was studied in fully kindled rats by measuring behavioral and electrophysiological parameters. Afferent vagus nerve activation was confirmed by quantifying nNOS immunoreactive cells in the nucleus of the solitary tract (NTS). Results: VNS rats had more nNOS immunoreactive cells/mm(2) in the NTS than shams. VNS induced a >25% decrease in stage 5 duration (S5D) in 32% of rats. Prior to VNS this type of responders suffered from seizures with a longer total seizure duration (TSD) than non-responders. In 21% of rats VNS resulted in a >25% decrease in TSD. This type of responders had a shorter TSD prior to VNS than non-responders. In 29% of rats VNS resulted in >200% increase in stage 5 latency (S5L). This type of responders had higher kindling rates than non-responders. Conclusion: The VNS-treated kindled rat is a clinically relevant animal model because it is a chronic epilepsy model that responds to VNS with effects that are comparable to the effects of VNS in epilepsy patients. In addition, this study demonstrates that VNS-treated kindled rats can be used to study the mode of action of VNS using immunohistochemical techniques. (C) 2010 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalBrain Research
Volume1319
Publication statusPublished - 10-Mar-2010
Externally publishedYes

    Keywords

  • Epilepsy, Seizure, Vagus nerve stimulation, Kindling, INTERMITTENT VAGAL-STIMULATION, DEEP BRAIN-STIMULATION, ELECTRICAL-STIMULATION, REFRACTORY EPILEPSY, INDUCTION, THERAPY, FOS, ELECTROACUPUNCTURE, IMMUNOREACTIVITY, EXPRESSION

ID: 130092558