Publication

Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice

De Smet, E., Mensink, R. P., Konings, M., Brufau Dones, G., Groen, A. K., Havinga, R., Schonewille, M., Kerksiek, A., Luetjohann, D. & Plat, J., Jun-2015, In : Lipids. 50, 6, p. 529-541 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

De Smet, E., Mensink, R. P., Konings, M., Brufau Dones, G., Groen, A. K., Havinga, R., Schonewille, M., Kerksiek, A., Luetjohann, D., & Plat, J. (2015). Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice. Lipids, 50(6), 529-541. https://doi.org/10.1007/s11745-015-4020-1

Author

De Smet, Els ; Mensink, Ronald P. ; Konings, Maurice ; Brufau Dones, Gemma ; Groen, Albert K. ; Havinga, Rick ; Schonewille, Marleen ; Kerksiek, Anja ; Luetjohann, Dieter ; Plat, Jogchum. / Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice. In: Lipids. 2015 ; Vol. 50, No. 6. pp. 529-541.

Harvard

De Smet, E, Mensink, RP, Konings, M, Brufau Dones, G, Groen, AK, Havinga, R, Schonewille, M, Kerksiek, A, Luetjohann, D & Plat, J 2015, 'Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice', Lipids, vol. 50, no. 6, pp. 529-541. https://doi.org/10.1007/s11745-015-4020-1

Standard

Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice. / De Smet, Els; Mensink, Ronald P.; Konings, Maurice; Brufau Dones, Gemma; Groen, Albert K.; Havinga, Rick; Schonewille, Marleen; Kerksiek, Anja; Luetjohann, Dieter; Plat, Jogchum.

In: Lipids, Vol. 50, No. 6, 06.2015, p. 529-541.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

De Smet E, Mensink RP, Konings M, Brufau Dones G, Groen AK, Havinga R et al. Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice. Lipids. 2015 Jun;50(6):529-541. https://doi.org/10.1007/s11745-015-4020-1


BibTeX

@article{09d55b44b1dd41968da1109636829ebe,
title = "Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice",
abstract = "The kinetics of plant stanol uptake and routing in 8-week-old C57BL/6J mice were determined after a plant stanol ester gavage. In addition, acute changes in intestinal and hepatic gene expression were investigated. Mice were fed a plant sterol/stanol poor diet from weaning. At the age of 8 weeks, they received an oral gavage consisting of 0.25 mg cholesterol + 50 mg plant stanol esters dissolved in olive oil. Animals were euthanized at different time points. In a second comparable set-up, mesenteric lymph-cannulated versus sham-operated mice received the same oral gavage, which was now deuterium labeled. Intestinal and hepatic sitostanol concentrations increased within 15 min post-gavage. This rapid hepatic appearance was absent in lymph-cannulated mice, suggesting a very fast lymph-mediated uptake. Hepatic mRNA expression of SREBP2 and its target genes rapidly decreased, whereas expression of LXR target genes increased. The intestinal SREBP2 pathway was increased, whereas the expression of LXR target genes hardly changed. The fivefold and sixfold increased expression of intestinal LDLr and PCSK9 is suggestive of TICE activation. We conclude that in C57BL/6J mice plant stanol kinetics are fast, and affect intestinal and hepatic gene expression within 15 min postprandial after lymph-mediated uptake.",
keywords = "Cholesterol, Gene expression, Lipids, Lipoproteins, Lymph canulation, Plant stanols, Intestines, CHOLESTEROL ABSORPTION, EZETIMIBE, SECRETION, STEROLS, HUMANS, PHYTOSTEROLS, TRANSPORTER, INHIBITION, REDUCTASE, ANTIBODY",
author = "{De Smet}, Els and Mensink, {Ronald P.} and Maurice Konings and {Brufau Dones}, Gemma and Groen, {Albert K.} and Rick Havinga and Marleen Schonewille and Anja Kerksiek and Dieter Luetjohann and Jogchum Plat",
year = "2015",
month = jun,
doi = "10.1007/s11745-015-4020-1",
language = "English",
volume = "50",
pages = "529--541",
journal = "Lipids",
issn = "1558-9307",
number = "6",

}

RIS

TY - JOUR

T1 - Acute Intake of Plant Stanol Esters Induces Changes in Lipid and Lipoprotein Metabolism-Related Gene Expression in the Liver and Intestines of Mice

AU - De Smet, Els

AU - Mensink, Ronald P.

AU - Konings, Maurice

AU - Brufau Dones, Gemma

AU - Groen, Albert K.

AU - Havinga, Rick

AU - Schonewille, Marleen

AU - Kerksiek, Anja

AU - Luetjohann, Dieter

AU - Plat, Jogchum

PY - 2015/6

Y1 - 2015/6

N2 - The kinetics of plant stanol uptake and routing in 8-week-old C57BL/6J mice were determined after a plant stanol ester gavage. In addition, acute changes in intestinal and hepatic gene expression were investigated. Mice were fed a plant sterol/stanol poor diet from weaning. At the age of 8 weeks, they received an oral gavage consisting of 0.25 mg cholesterol + 50 mg plant stanol esters dissolved in olive oil. Animals were euthanized at different time points. In a second comparable set-up, mesenteric lymph-cannulated versus sham-operated mice received the same oral gavage, which was now deuterium labeled. Intestinal and hepatic sitostanol concentrations increased within 15 min post-gavage. This rapid hepatic appearance was absent in lymph-cannulated mice, suggesting a very fast lymph-mediated uptake. Hepatic mRNA expression of SREBP2 and its target genes rapidly decreased, whereas expression of LXR target genes increased. The intestinal SREBP2 pathway was increased, whereas the expression of LXR target genes hardly changed. The fivefold and sixfold increased expression of intestinal LDLr and PCSK9 is suggestive of TICE activation. We conclude that in C57BL/6J mice plant stanol kinetics are fast, and affect intestinal and hepatic gene expression within 15 min postprandial after lymph-mediated uptake.

AB - The kinetics of plant stanol uptake and routing in 8-week-old C57BL/6J mice were determined after a plant stanol ester gavage. In addition, acute changes in intestinal and hepatic gene expression were investigated. Mice were fed a plant sterol/stanol poor diet from weaning. At the age of 8 weeks, they received an oral gavage consisting of 0.25 mg cholesterol + 50 mg plant stanol esters dissolved in olive oil. Animals were euthanized at different time points. In a second comparable set-up, mesenteric lymph-cannulated versus sham-operated mice received the same oral gavage, which was now deuterium labeled. Intestinal and hepatic sitostanol concentrations increased within 15 min post-gavage. This rapid hepatic appearance was absent in lymph-cannulated mice, suggesting a very fast lymph-mediated uptake. Hepatic mRNA expression of SREBP2 and its target genes rapidly decreased, whereas expression of LXR target genes increased. The intestinal SREBP2 pathway was increased, whereas the expression of LXR target genes hardly changed. The fivefold and sixfold increased expression of intestinal LDLr and PCSK9 is suggestive of TICE activation. We conclude that in C57BL/6J mice plant stanol kinetics are fast, and affect intestinal and hepatic gene expression within 15 min postprandial after lymph-mediated uptake.

KW - Cholesterol

KW - Gene expression

KW - Lipids

KW - Lipoproteins

KW - Lymph canulation

KW - Plant stanols

KW - Intestines

KW - CHOLESTEROL ABSORPTION

KW - EZETIMIBE

KW - SECRETION

KW - STEROLS

KW - HUMANS

KW - PHYTOSTEROLS

KW - TRANSPORTER

KW - INHIBITION

KW - REDUCTASE

KW - ANTIBODY

U2 - 10.1007/s11745-015-4020-1

DO - 10.1007/s11745-015-4020-1

M3 - Article

VL - 50

SP - 529

EP - 541

JO - Lipids

JF - Lipids

SN - 1558-9307

IS - 6

ER -

ID: 20896020