Publication

Active probing of the mechanical properties of biological and synthetic vesicles

Piontek, M. C., Lira, R. B. & Roos, W. H., 14-Nov-2019, In : Biochimica et Biophysica Acta-General Subjects. 15 p., 129486.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Piontek, M. C., Lira, R. B., & Roos, W. H. (2019). Active probing of the mechanical properties of biological and synthetic vesicles. Biochimica et Biophysica Acta-General Subjects, [129486]. https://doi.org/10.1016/j.bbagen.2019.129486

Author

Piontek, Melissa C. ; Lira, Rafael B. ; Roos, Wouter H. / Active probing of the mechanical properties of biological and synthetic vesicles. In: Biochimica et Biophysica Acta-General Subjects. 2019.

Harvard

Piontek, MC, Lira, RB & Roos, WH 2019, 'Active probing of the mechanical properties of biological and synthetic vesicles', Biochimica et Biophysica Acta-General Subjects. https://doi.org/10.1016/j.bbagen.2019.129486

Standard

Active probing of the mechanical properties of biological and synthetic vesicles. / Piontek, Melissa C.; Lira, Rafael B.; Roos, Wouter H.

In: Biochimica et Biophysica Acta-General Subjects, 14.11.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Piontek MC, Lira RB, Roos WH. Active probing of the mechanical properties of biological and synthetic vesicles. Biochimica et Biophysica Acta-General Subjects. 2019 Nov 14. 129486. https://doi.org/10.1016/j.bbagen.2019.129486


BibTeX

@article{d860cd87ef1f497bbe52012f1aa9a62b,
title = "Active probing of the mechanical properties of biological and synthetic vesicles",
abstract = "BACKGROUND: The interest in mechanics of synthetic and biological vesicles has been continuously growing during the last decades. Liposomes serve as model systems for investigating fundamental membrane processes and properties. More recently, extracellular vesicles (EVs) have been investigated mechanically as well. EVs are widely studied in fundamental and applied sciences, but their material properties remained elusive until recently. Elucidating the mechanical properties of vesicles is essential to unveil the mechanisms behind a variety of biological processes, e.g. budding, vesiculation and cellular uptake mechanisms. SCOPE OF REVIEW: The importance of mechanobiology for studies of vesicles and membranes is discussed, as well as the different available techniques to probe their mechanical properties. In particular, the mechanics of vesicles and membranes as obtained by nanoindentation, micropipette aspiration, optical tweezers, electrodeformation and electroporation experiments is addressed. MAJOR CONCLUSIONS: EVs and liposomes possess an astonishing rich, diverse behavior. To better understand their properties, and for optimization of their applications in nanotechnology, an improved understanding of their mechanical properties is needed. Depending on the size of the vesicles and the specific scientific question, different techniques can be chosen for their mechanical characterization. GENERAL SIGNIFICANCE: Understanding the mechanical properties of vesicles is necessary to gain deeper insight in the fundamental biological mechanisms involved in vesicle generation and cellular uptake. This furthermore facilitates technological applications such as using vesicles as targeted drug delivery vehicles. Liposome studies provide insight into fundamental membrane processes and properties, whereas the role and functioning of EVs in biology and medicine is increasingly elucidated.",
keywords = "Atomic force microscopy (AFM), Electrodeformation, Mechanical properties, Micropipette aspiration (MPA), Optical tweezers (OT), Vesicles, article, aspiration, atomic force microscopy, budding, controlled study, electroporation, exosome, mechanics, membrane biology, micropipette, nanotechnology, optical tweezers, liposome",
author = "Piontek, {Melissa C.} and Lira, {Rafael B.} and Roos, {Wouter H.}",
year = "2019",
month = "11",
day = "14",
doi = "10.1016/j.bbagen.2019.129486",
language = "English",
journal = "Biochimica et Biophysica Acta-General Subjects",
issn = "0304-4165",
publisher = "ELSEVIER SCIENCE BV",

}

RIS

TY - JOUR

T1 - Active probing of the mechanical properties of biological and synthetic vesicles

AU - Piontek, Melissa C.

AU - Lira, Rafael B.

AU - Roos, Wouter H.

PY - 2019/11/14

Y1 - 2019/11/14

N2 - BACKGROUND: The interest in mechanics of synthetic and biological vesicles has been continuously growing during the last decades. Liposomes serve as model systems for investigating fundamental membrane processes and properties. More recently, extracellular vesicles (EVs) have been investigated mechanically as well. EVs are widely studied in fundamental and applied sciences, but their material properties remained elusive until recently. Elucidating the mechanical properties of vesicles is essential to unveil the mechanisms behind a variety of biological processes, e.g. budding, vesiculation and cellular uptake mechanisms. SCOPE OF REVIEW: The importance of mechanobiology for studies of vesicles and membranes is discussed, as well as the different available techniques to probe their mechanical properties. In particular, the mechanics of vesicles and membranes as obtained by nanoindentation, micropipette aspiration, optical tweezers, electrodeformation and electroporation experiments is addressed. MAJOR CONCLUSIONS: EVs and liposomes possess an astonishing rich, diverse behavior. To better understand their properties, and for optimization of their applications in nanotechnology, an improved understanding of their mechanical properties is needed. Depending on the size of the vesicles and the specific scientific question, different techniques can be chosen for their mechanical characterization. GENERAL SIGNIFICANCE: Understanding the mechanical properties of vesicles is necessary to gain deeper insight in the fundamental biological mechanisms involved in vesicle generation and cellular uptake. This furthermore facilitates technological applications such as using vesicles as targeted drug delivery vehicles. Liposome studies provide insight into fundamental membrane processes and properties, whereas the role and functioning of EVs in biology and medicine is increasingly elucidated.

AB - BACKGROUND: The interest in mechanics of synthetic and biological vesicles has been continuously growing during the last decades. Liposomes serve as model systems for investigating fundamental membrane processes and properties. More recently, extracellular vesicles (EVs) have been investigated mechanically as well. EVs are widely studied in fundamental and applied sciences, but their material properties remained elusive until recently. Elucidating the mechanical properties of vesicles is essential to unveil the mechanisms behind a variety of biological processes, e.g. budding, vesiculation and cellular uptake mechanisms. SCOPE OF REVIEW: The importance of mechanobiology for studies of vesicles and membranes is discussed, as well as the different available techniques to probe their mechanical properties. In particular, the mechanics of vesicles and membranes as obtained by nanoindentation, micropipette aspiration, optical tweezers, electrodeformation and electroporation experiments is addressed. MAJOR CONCLUSIONS: EVs and liposomes possess an astonishing rich, diverse behavior. To better understand their properties, and for optimization of their applications in nanotechnology, an improved understanding of their mechanical properties is needed. Depending on the size of the vesicles and the specific scientific question, different techniques can be chosen for their mechanical characterization. GENERAL SIGNIFICANCE: Understanding the mechanical properties of vesicles is necessary to gain deeper insight in the fundamental biological mechanisms involved in vesicle generation and cellular uptake. This furthermore facilitates technological applications such as using vesicles as targeted drug delivery vehicles. Liposome studies provide insight into fundamental membrane processes and properties, whereas the role and functioning of EVs in biology and medicine is increasingly elucidated.

KW - Atomic force microscopy (AFM)

KW - Electrodeformation

KW - Mechanical properties

KW - Micropipette aspiration (MPA)

KW - Optical tweezers (OT)

KW - Vesicles

KW - article

KW - aspiration

KW - atomic force microscopy

KW - budding

KW - controlled study

KW - electroporation

KW - exosome

KW - mechanics

KW - membrane biology

KW - micropipette

KW - nanotechnology

KW - optical tweezers

KW - liposome

U2 - 10.1016/j.bbagen.2019.129486

DO - 10.1016/j.bbagen.2019.129486

M3 - Article

JO - Biochimica et Biophysica Acta-General Subjects

JF - Biochimica et Biophysica Acta-General Subjects

SN - 0304-4165

M1 - 129486

ER -

ID: 109491632