Publication

Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers

Karangwa, S. A., Burlage, L. C., Adelmeijer, J., Karimian, N., Westerkamp, A. C., Matton, A. P., van Rijn, R., Wiersema-Buist, J., Sutton, M. E., op den Dries, S., Lisman, T. & Porte, R. J., Feb-2017, In : Transplantation. 101, 2, p. E42-E48 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Karangwa, S. A., Burlage, L. C., Adelmeijer, J., Karimian, N., Westerkamp, A. C., Matton, A. P., ... Porte, R. J. (2017). Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers. Transplantation, 101(2), E42-E48. https://doi.org/10.1097/TP.0000000000001562

Author

Karangwa, Shanice A. ; Burlage, Laura C. ; Adelmeijer, Jelle ; Karimian, Negin ; Westerkamp, Andrie C. ; Matton, Alix P. ; van Rijn, Rianne ; Wiersema-Buist, Janneke ; Sutton, Michael E. ; op den Dries, Sanna ; Lisman, Ton ; Porte, Robert J. / Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers. In: Transplantation. 2017 ; Vol. 101, No. 2. pp. E42-E48.

Harvard

Karangwa, SA, Burlage, LC, Adelmeijer, J, Karimian, N, Westerkamp, AC, Matton, AP, van Rijn, R, Wiersema-Buist, J, Sutton, ME, op den Dries, S, Lisman, T & Porte, RJ 2017, 'Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers', Transplantation, vol. 101, no. 2, pp. E42-E48. https://doi.org/10.1097/TP.0000000000001562

Standard

Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers. / Karangwa, Shanice A.; Burlage, Laura C.; Adelmeijer, Jelle; Karimian, Negin; Westerkamp, Andrie C.; Matton, Alix P.; van Rijn, Rianne; Wiersema-Buist, Janneke; Sutton, Michael E.; op den Dries, Sanna; Lisman, Ton; Porte, Robert J.

In: Transplantation, Vol. 101, No. 2, 02.2017, p. E42-E48.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Karangwa SA, Burlage LC, Adelmeijer J, Karimian N, Westerkamp AC, Matton AP et al. Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers. Transplantation. 2017 Feb;101(2):E42-E48. https://doi.org/10.1097/TP.0000000000001562


BibTeX

@article{b058f073cd6a46e6a632f40fca9aaf8c,
title = "Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers",
abstract = "Background. Ex situ normothermic machine perfusion (NMP) can be performed after traditional static cold preservation to assess graft function and viability before transplantation. It is unknown whether this results in activation of coagulation and fibrinolysis, as may occur upon graft reperfusion in vivo. Methods. Twelve donor livers declined for transplantation underwent 6 hours of end-ischemic NMP using a heparinized plasma-based perfusion fluid. Concentration of prothrombin fragment F1 + 2 (marker of coagulation activation), D-dimer, plasmin-antiplasmin complex, tissue plasminogen activator and plasminogen activator inhibitor-1 (markers for fibrinolysis) and alanine aminotransferase (ALT) (marker of ischemia-reperfusion [I/R] injury) were measured in perfusion fluid at regular intervals. Liver biopsies were examined for the presence of fibrin, using light microscopy after Maurits, Scarlet and Blue staining. Results. No significant increase in prothrombin F1 + 2 was noted during NMP. D-dimer and plasmin-antiplasmin complex levels increased soon after start of NMP and D-dimer concentrations correlated significantly with levels of tissue plasminogen activator. In livers displaying good function during NMP, perfusate levels of ALT and D-dimers were low (3500 ng/mL) were in found in livers with poor graft function. Activation of fibrinolysis correlated significantly with the degree of I/R injury, as reflected by ALT levels. Conclusions. End-ischemic ex situ NMP results in activation of fibrinolysis, but not of coagulation. Markers of fibrinolysis activation correlate significantly with markers of I/R injury. High concentrations of D-dimer early after start of NMP can be considered a marker of severe I/R injury and a predictor of poor liver graft function.",
keywords = "TISSUE-PLASMINOGEN ACTIVATOR, CIRCULATORY DEATH DONORS, CARDIAC DEATH, BILIARY COMPLICATIONS, COLD-STORAGE, DCD LIVERS, TRANSPLANTATION, PRESERVATION, DONATION, INJURY",
author = "Karangwa, {Shanice A.} and Burlage, {Laura C.} and Jelle Adelmeijer and Negin Karimian and Westerkamp, {Andrie C.} and Matton, {Alix P.} and {van Rijn}, Rianne and Janneke Wiersema-Buist and Sutton, {Michael E.} and {op den Dries}, Sanna and Ton Lisman and Porte, {Robert J.}",
year = "2017",
month = "2",
doi = "10.1097/TP.0000000000001562",
language = "English",
volume = "101",
pages = "E42--E48",
journal = "Transplantation",
issn = "0041-1337",
publisher = "LIPPINCOTT WILLIAMS & WILKINS",
number = "2",

}

RIS

TY - JOUR

T1 - Activation of Fibrinolysis, But Not Coagulation, During End-Ischemic Ex Situ Normothermic Machine Perfusion of Human Donor Livers

AU - Karangwa, Shanice A.

AU - Burlage, Laura C.

AU - Adelmeijer, Jelle

AU - Karimian, Negin

AU - Westerkamp, Andrie C.

AU - Matton, Alix P.

AU - van Rijn, Rianne

AU - Wiersema-Buist, Janneke

AU - Sutton, Michael E.

AU - op den Dries, Sanna

AU - Lisman, Ton

AU - Porte, Robert J.

PY - 2017/2

Y1 - 2017/2

N2 - Background. Ex situ normothermic machine perfusion (NMP) can be performed after traditional static cold preservation to assess graft function and viability before transplantation. It is unknown whether this results in activation of coagulation and fibrinolysis, as may occur upon graft reperfusion in vivo. Methods. Twelve donor livers declined for transplantation underwent 6 hours of end-ischemic NMP using a heparinized plasma-based perfusion fluid. Concentration of prothrombin fragment F1 + 2 (marker of coagulation activation), D-dimer, plasmin-antiplasmin complex, tissue plasminogen activator and plasminogen activator inhibitor-1 (markers for fibrinolysis) and alanine aminotransferase (ALT) (marker of ischemia-reperfusion [I/R] injury) were measured in perfusion fluid at regular intervals. Liver biopsies were examined for the presence of fibrin, using light microscopy after Maurits, Scarlet and Blue staining. Results. No significant increase in prothrombin F1 + 2 was noted during NMP. D-dimer and plasmin-antiplasmin complex levels increased soon after start of NMP and D-dimer concentrations correlated significantly with levels of tissue plasminogen activator. In livers displaying good function during NMP, perfusate levels of ALT and D-dimers were low (3500 ng/mL) were in found in livers with poor graft function. Activation of fibrinolysis correlated significantly with the degree of I/R injury, as reflected by ALT levels. Conclusions. End-ischemic ex situ NMP results in activation of fibrinolysis, but not of coagulation. Markers of fibrinolysis activation correlate significantly with markers of I/R injury. High concentrations of D-dimer early after start of NMP can be considered a marker of severe I/R injury and a predictor of poor liver graft function.

AB - Background. Ex situ normothermic machine perfusion (NMP) can be performed after traditional static cold preservation to assess graft function and viability before transplantation. It is unknown whether this results in activation of coagulation and fibrinolysis, as may occur upon graft reperfusion in vivo. Methods. Twelve donor livers declined for transplantation underwent 6 hours of end-ischemic NMP using a heparinized plasma-based perfusion fluid. Concentration of prothrombin fragment F1 + 2 (marker of coagulation activation), D-dimer, plasmin-antiplasmin complex, tissue plasminogen activator and plasminogen activator inhibitor-1 (markers for fibrinolysis) and alanine aminotransferase (ALT) (marker of ischemia-reperfusion [I/R] injury) were measured in perfusion fluid at regular intervals. Liver biopsies were examined for the presence of fibrin, using light microscopy after Maurits, Scarlet and Blue staining. Results. No significant increase in prothrombin F1 + 2 was noted during NMP. D-dimer and plasmin-antiplasmin complex levels increased soon after start of NMP and D-dimer concentrations correlated significantly with levels of tissue plasminogen activator. In livers displaying good function during NMP, perfusate levels of ALT and D-dimers were low (3500 ng/mL) were in found in livers with poor graft function. Activation of fibrinolysis correlated significantly with the degree of I/R injury, as reflected by ALT levels. Conclusions. End-ischemic ex situ NMP results in activation of fibrinolysis, but not of coagulation. Markers of fibrinolysis activation correlate significantly with markers of I/R injury. High concentrations of D-dimer early after start of NMP can be considered a marker of severe I/R injury and a predictor of poor liver graft function.

KW - TISSUE-PLASMINOGEN ACTIVATOR

KW - CIRCULATORY DEATH DONORS

KW - CARDIAC DEATH

KW - BILIARY COMPLICATIONS

KW - COLD-STORAGE

KW - DCD LIVERS

KW - TRANSPLANTATION

KW - PRESERVATION

KW - DONATION

KW - INJURY

U2 - 10.1097/TP.0000000000001562

DO - 10.1097/TP.0000000000001562

M3 - Article

C2 - 27941437

VL - 101

SP - E42-E48

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 2

ER -

ID: 42187110