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Acquisition of the ability for Rhodopseudomonas palustris to degrade chlorinated benzoic acids as the sole carbon source

Oda, Y., de Vries, YP., Forney, LJ. & Gottschal, JC., 26-Dec-2001, In : FEMS Microbial Ecology. 38, 2-3, p. 133-139 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Y Oda
  • YP de Vries
  • LJ Forney
  • JC Gottschal

Three strains of Rhodopseudomonas palustris were isolated from phototrophic enrichment cultures containing 3-chlorobenzoate (3-CBA) and benzoate (BA). These new strains as well as several previously described strains of R. palustris were tested in this study and shown to degrade 3-CBA if grown in media that contained BA as a co-substrate. All of the pure cultures that originally required BA for the degradation of 3-CBA acquired the ability to degrade 3-CBA as the sole carbon source after long periods of incubation that ranged from I to 3 months. After this adaptation period, the 3-CBA-degrading capabilities of all variants were stable. and the rates of 3-CBA degradation were significantly enhanced as compared to the parental strains. Furthermore, the variants had also acquired the ability to metabolize 2- and 4-CBA as sole carbon sources indicating that the enhanced ability to metabolize 3-CBA was accompanied by an expanded ability to metabolize chlorinated benzoates. These data indicate that acquisition of the ability to degrade 3-CBA may be rather common among strains of R. palustris and mutations that confer the ability to metabolize 3-CBA may provide a selective advantage to R. palustris under specific environmental conditions. (C) 2001 Federation of European Microbiological Societies, Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalFEMS Microbial Ecology
Volume38
Issue number2-3
Publication statusPublished - 26-Dec-2001

    Keywords

  • phototrophic enrichnient, 3-chlorobenzoate, adaptation, Rhodopseudomonas palustris, XENOBIOTIC COMPOUNDS, AROMATIC-COMPOUNDS, MICROBIAL COMMUNITIES, BIODEGRADATION, ADAPTATION, METABOLISM, EVOLUTION, COENZYME, MUTATION, BACTERIA

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