A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and α-hydroxymetoprolol in human serum for application in pharmacokinetics.Postma-Kunnen, S., Yska, J. P., Hommema, G., Koopmans, S., Wilffert, B. & van Roon, E., Jun-2017, In : Journal of Applied Bioanalysis. 3, 3, p. 49-57 9 p.
Research output: Contribution to journal › Article › Academic › peer-review
To determine metoprolol and its metabolite α-hydroxymetoprolol in human serum we validated a method on an LC system with an Exactive® Orbitrap mass spectrometer (Thermo Scientific) as detector and isotope-labelled metoprolol-d7 as internal standard. A simple sample preparation was used with water-acetonitrile (15:85, v/v) as precipitation reagent. This method has a chromatographic run time of 15 min and linear calibration curves in the range of 5.0-250 µg/L for both metoprolol and α-hydroxymetoprolol. Validation showed the method to be accurate, with a good precision, selective and with a lower limit of quantitation of 2.0 µg/L for metoprolol and 1.0 µg/L for α-hydroxymetoprolol, respectively. This validated LC-Orbitrap MS analysis for metoprolol and α-hydroxymetoprolol can be used for application in human pharmacokinetics.
|Number of pages||9|
|Journal||Journal of Applied Bioanalysis|
|Publication status||Published - Jun-2017|
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