Publication

A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer

Workel, H. H., Lubbers, J. M., Arnold, R., Prins, T. M., van der Vlies, P., de Lange, K., Bosse, T., Van Gool, I. C., Eggink, F. A., Wouters, M. C., Komdeur, F. L., van der Slikke, E. C., Creutzberg, C. L., Kol, A., Plat, A., Glaire, M., Church, D. N., Nijman, H. W. & de Bruyn, M., May-2019, In : Cancer immunology research. 7, 5, p. 784-796 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Workel, H. H., Lubbers, J. M., Arnold, R., Prins, T. M., van der Vlies, P., de Lange, K., Bosse, T., Van Gool, I. C., Eggink, F. A., Wouters, M. C., Komdeur, F. L., van der Slikke, E. C., Creutzberg, C. L., Kol, A., Plat, A., Glaire, M., Church, D. N., Nijman, H. W., & de Bruyn, M. (2019). A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer. Cancer immunology research, 7(5), 784-796. https://doi.org/10.1158/2326-6066.CIR-18-0517

Author

Workel, Hagma H ; Lubbers, Joyce M ; Arnold, Roland ; Prins, Thalina M ; van der Vlies, Pieter ; de Lange, Kim ; Bosse, Tjalling ; Van Gool, Inge C ; Eggink, Florine A ; Wouters, Maartje Ca ; Komdeur, Fenne L ; van der Slikke, Elisabeth C ; Creutzberg, Carien L ; Kol, Arjan ; Plat, Annechien ; Glaire, Mark ; Church, David N ; Nijman, Hans W ; de Bruyn, Marco. / A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer. In: Cancer immunology research. 2019 ; Vol. 7, No. 5. pp. 784-796.

Harvard

Workel, HH, Lubbers, JM, Arnold, R, Prins, TM, van der Vlies, P, de Lange, K, Bosse, T, Van Gool, IC, Eggink, FA, Wouters, MC, Komdeur, FL, van der Slikke, EC, Creutzberg, CL, Kol, A, Plat, A, Glaire, M, Church, DN, Nijman, HW & de Bruyn, M 2019, 'A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer', Cancer immunology research, vol. 7, no. 5, pp. 784-796. https://doi.org/10.1158/2326-6066.CIR-18-0517

Standard

A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer. / Workel, Hagma H; Lubbers, Joyce M; Arnold, Roland; Prins, Thalina M; van der Vlies, Pieter; de Lange, Kim; Bosse, Tjalling; Van Gool, Inge C; Eggink, Florine A; Wouters, Maartje Ca; Komdeur, Fenne L; van der Slikke, Elisabeth C; Creutzberg, Carien L; Kol, Arjan; Plat, Annechien; Glaire, Mark; Church, David N; Nijman, Hans W; de Bruyn, Marco.

In: Cancer immunology research, Vol. 7, No. 5, 05.2019, p. 784-796.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Workel HH, Lubbers JM, Arnold R, Prins TM, van der Vlies P, de Lange K et al. A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer. Cancer immunology research. 2019 May;7(5):784-796. https://doi.org/10.1158/2326-6066.CIR-18-0517


BibTeX

@article{692ac47b56334f7aafef7ac3414120a4,
title = "A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer",
abstract = "The chemokine CXCL13 mediates recruitment of B cells to tumors and is essential for the formation of tertiary lymphoid structures (TLSs). TLSs are thought to support antitumor immunity and are associated with improved prognosis. However, it remains unknown whether TLSs are formed in response to the general inflammatory character of the tumor microenvironment, or rather, are induced by (neo)antigen-specific adaptive immunity. We here report on the finding that the transforming growth factor beta (TGFβ)-dependent CD103+CD8+ tumor-infiltrating T-cell (TIL) subpopulation expressed and produced CXCL13. Accordingly, CD8+ T cells from peripheral blood activated in the presence of TGFβ upregulated CD103 and secreted CXCL13. Conversely, inhibition of TGFβ receptor signaling abrogated CXCL13 production. CXCL13+CD103+CD8+ TILs correlated with B-cell recruitment, TLSs, and neoantigen burden in six cohorts of human tumors. Altogether, our findings indicated that TGFβ plays a non-canonical role in coordinating immune responses against human tumors and suggest a potential role for CXCL13+CD103+CD8+ TILs in mediating B-cell recruitment and TLS formation in human tumors.",
author = "Workel, {Hagma H} and Lubbers, {Joyce M} and Roland Arnold and Prins, {Thalina M} and {van der Vlies}, Pieter and {de Lange}, Kim and Tjalling Bosse and {Van Gool}, {Inge C} and Eggink, {Florine A} and Wouters, {Maartje Ca} and Komdeur, {Fenne L} and {van der Slikke}, {Elisabeth C} and Creutzberg, {Carien L} and Arjan Kol and Annechien Plat and Mark Glaire and Church, {David N} and Nijman, {Hans W} and {de Bruyn}, Marco",
note = "Copyright {\textcopyright}2019, American Association for Cancer Research.",
year = "2019",
month = may,
doi = "10.1158/2326-6066.CIR-18-0517",
language = "English",
volume = "7",
pages = "784--796",
journal = "Cancer immunology research",
issn = "2326-6066",
publisher = "AMER ASSOC CANCER RESEARCH",
number = "5",

}

RIS

TY - JOUR

T1 - A transcriptionally distinct CXCL13+CD103+CD8+ T-cell population is associated with B-cell recruitment and neoantigen load in human cancer

AU - Workel, Hagma H

AU - Lubbers, Joyce M

AU - Arnold, Roland

AU - Prins, Thalina M

AU - van der Vlies, Pieter

AU - de Lange, Kim

AU - Bosse, Tjalling

AU - Van Gool, Inge C

AU - Eggink, Florine A

AU - Wouters, Maartje Ca

AU - Komdeur, Fenne L

AU - van der Slikke, Elisabeth C

AU - Creutzberg, Carien L

AU - Kol, Arjan

AU - Plat, Annechien

AU - Glaire, Mark

AU - Church, David N

AU - Nijman, Hans W

AU - de Bruyn, Marco

N1 - Copyright ©2019, American Association for Cancer Research.

PY - 2019/5

Y1 - 2019/5

N2 - The chemokine CXCL13 mediates recruitment of B cells to tumors and is essential for the formation of tertiary lymphoid structures (TLSs). TLSs are thought to support antitumor immunity and are associated with improved prognosis. However, it remains unknown whether TLSs are formed in response to the general inflammatory character of the tumor microenvironment, or rather, are induced by (neo)antigen-specific adaptive immunity. We here report on the finding that the transforming growth factor beta (TGFβ)-dependent CD103+CD8+ tumor-infiltrating T-cell (TIL) subpopulation expressed and produced CXCL13. Accordingly, CD8+ T cells from peripheral blood activated in the presence of TGFβ upregulated CD103 and secreted CXCL13. Conversely, inhibition of TGFβ receptor signaling abrogated CXCL13 production. CXCL13+CD103+CD8+ TILs correlated with B-cell recruitment, TLSs, and neoantigen burden in six cohorts of human tumors. Altogether, our findings indicated that TGFβ plays a non-canonical role in coordinating immune responses against human tumors and suggest a potential role for CXCL13+CD103+CD8+ TILs in mediating B-cell recruitment and TLS formation in human tumors.

AB - The chemokine CXCL13 mediates recruitment of B cells to tumors and is essential for the formation of tertiary lymphoid structures (TLSs). TLSs are thought to support antitumor immunity and are associated with improved prognosis. However, it remains unknown whether TLSs are formed in response to the general inflammatory character of the tumor microenvironment, or rather, are induced by (neo)antigen-specific adaptive immunity. We here report on the finding that the transforming growth factor beta (TGFβ)-dependent CD103+CD8+ tumor-infiltrating T-cell (TIL) subpopulation expressed and produced CXCL13. Accordingly, CD8+ T cells from peripheral blood activated in the presence of TGFβ upregulated CD103 and secreted CXCL13. Conversely, inhibition of TGFβ receptor signaling abrogated CXCL13 production. CXCL13+CD103+CD8+ TILs correlated with B-cell recruitment, TLSs, and neoantigen burden in six cohorts of human tumors. Altogether, our findings indicated that TGFβ plays a non-canonical role in coordinating immune responses against human tumors and suggest a potential role for CXCL13+CD103+CD8+ TILs in mediating B-cell recruitment and TLS formation in human tumors.

U2 - 10.1158/2326-6066.CIR-18-0517

DO - 10.1158/2326-6066.CIR-18-0517

M3 - Article

C2 - 30872264

VL - 7

SP - 784

EP - 796

JO - Cancer immunology research

JF - Cancer immunology research

SN - 2326-6066

IS - 5

ER -

ID: 78385782