Publication

A Tool for Alignment and Averaging of Sparse Fluorescence Signals in Rod-Shaped Bacteria

Goudsmits, J. M. H., van Oijen, A. M. & Robinson, A., 26-Apr-2016, In : Biophysical Journal. 110, 8, p. 1708-1715 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

Fluorescence microscopy studies have shown that many proteins localize to highly specific subregions within bacterial cells. Analyzing the spatial distribution of low-abundance proteins within cells is highly challenging because information obtained from multiple cells needs to be combined to provide well-defined maps of protein locations. We present (to our knowledge) a novel tool for fast, automated, and user-impartial analysis of fluorescent protein distribution across the short axis of rod-shaped bacteria. To demonstrate the strength of our approach in extracting spatial distributions and visualizing dynamic intracellular processes, we analyzed sparse fluorescence signals from single-molecule time-lapse images of individual Escherichia coli cells. In principle, our tool can be used to provide information on the distribution of signal intensity across the short axis of any rod-shaped object.

Original languageEnglish
Pages (from-to)1708-1715
Number of pages8
JournalBiophysical Journal
Volume110
Issue number8
Publication statusPublished - 26-Apr-2016

    Keywords

  • HIGH-THROUGHPUT, IMAGE-ANALYSIS, PROTEIN LOCALIZATION, ANALYSIS SOFTWARE, ESCHERICHIA-COLI, DYNAMICS, MORPHOGENESIS, RING

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