A Tool for Alignment and Averaging of Sparse Fluorescence Signals in Rod-Shaped BacteriaGoudsmits, J. M. H., van Oijen, A. M. & Robinson, A., 26-Apr-2016, In : Biophysical Journal. 110, 8, p. 1708-1715 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Fluorescence microscopy studies have shown that many proteins localize to highly specific subregions within bacterial cells. Analyzing the spatial distribution of low-abundance proteins within cells is highly challenging because information obtained from multiple cells needs to be combined to provide well-defined maps of protein locations. We present (to our knowledge) a novel tool for fast, automated, and user-impartial analysis of fluorescent protein distribution across the short axis of rod-shaped bacteria. To demonstrate the strength of our approach in extracting spatial distributions and visualizing dynamic intracellular processes, we analyzed sparse fluorescence signals from single-molecule time-lapse images of individual Escherichia coli cells. In principle, our tool can be used to provide information on the distribution of signal intensity across the short axis of any rod-shaped object.
|Number of pages||8|
|Publication status||Published - 26-Apr-2016|
- HIGH-THROUGHPUT, IMAGE-ANALYSIS, PROTEIN LOCALIZATION, ANALYSIS SOFTWARE, ESCHERICHIA-COLI, DYNAMICS, MORPHOGENESIS, RING