A single dose of granulocyte-macrophage colony-stimulating factor induces systemic interleukin-8 release and neutrophil activation in healthy volunteersvan Pelt, L. J., Huisman, M. V., Weening, R. S., von dem Borne, A. E., Roos, D. & van Oers, R. H., 15-Jun-1996, In : Blood. 87, 12, p. 5305-5313 9 p.
Research output: Contribution to journal › Article › Academic › peer-review
Granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) are frequently used in the clinical management of neutropenia. These cytokines not only enhance the proliferation of myeloid precursor cells but also influence the function of mature leukocytes. In a previous study, we found that the in vivo effects of G-CSF on neutrophils differed from those in vitro. In the present study, we investigated the effects of a single dose of recombinant GM-CSF (7.5 microg/kg, subcutaneously) on neutrophils, eosinophils, and monocytes in healthy volunteers. We analyzed leukocyte kinetics, phenotypical changes, neutrophil degranulation, and systemic cytokine production. After GM-CSF injection, phenotypical changes included upregulation of CD11b on all three cell types and a decreased expression of L-selectin and Fc(gamma)RIII on neutrophils. Neutrophil degranulation was evident from the increased plasma concentrations of lactoferrin and elastase. GM-CSF induced the release of interleukin-8 (IL-8), but not of IL-6 or tumor necrosis factor alpha. In comparison to the results from our previous study with G-CSF in healthy volunteers, GM-CSF induced a stronger activation of mature neutrophils but had a much less pronounced effect on the production and maturation of neutrophil precursors. These data may help to guide the choice between the two cytokines in different clinical situations.
|Number of pages||9|
|Publication status||Published - 15-Jun-1996|
- Adult, Cytoplasmic Granules/metabolism, Eosinophils/drug effects, Gene Expression Regulation/drug effects, Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage, Humans, Immunophenotyping, Injections, Subcutaneous, Interleukin-6/metabolism, Interleukin-8/metabolism, Kinetics, L-Selectin/biosynthesis, Lactoferrin/metabolism, Leukocyte Count/drug effects, Leukocyte Elastase, Macrophage-1 Antigen/biosynthesis, Male, Monocytes/drug effects, Neutrophils/drug effects, Pancreatic Elastase/metabolism, Receptors, IgG/biosynthesis, Recombinant Proteins/administration & dosage, Tumor Necrosis Factor-alpha/metabolism