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A single dose of granulocyte-macrophage colony-stimulating factor induces systemic interleukin-8 release and neutrophil activation in healthy volunteers

van Pelt, L. J., Huisman, M. V., Weening, R. S., von dem Borne, A. E., Roos, D. & van Oers, R. H., 15-Jun-1996, In : Blood. 87, 12, p. 5305-5313 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • L J van Pelt
  • M V Huisman
  • R S Weening
  • A E von dem Borne
  • D Roos
  • R H van Oers

Granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) are frequently used in the clinical management of neutropenia. These cytokines not only enhance the proliferation of myeloid precursor cells but also influence the function of mature leukocytes. In a previous study, we found that the in vivo effects of G-CSF on neutrophils differed from those in vitro. In the present study, we investigated the effects of a single dose of recombinant GM-CSF (7.5 microg/kg, subcutaneously) on neutrophils, eosinophils, and monocytes in healthy volunteers. We analyzed leukocyte kinetics, phenotypical changes, neutrophil degranulation, and systemic cytokine production. After GM-CSF injection, phenotypical changes included upregulation of CD11b on all three cell types and a decreased expression of L-selectin and Fc(gamma)RIII on neutrophils. Neutrophil degranulation was evident from the increased plasma concentrations of lactoferrin and elastase. GM-CSF induced the release of interleukin-8 (IL-8), but not of IL-6 or tumor necrosis factor alpha. In comparison to the results from our previous study with G-CSF in healthy volunteers, GM-CSF induced a stronger activation of mature neutrophils but had a much less pronounced effect on the production and maturation of neutrophil precursors. These data may help to guide the choice between the two cytokines in different clinical situations.

Original languageEnglish
Pages (from-to)5305-5313
Number of pages9
JournalBlood
Volume87
Issue number12
Publication statusPublished - 15-Jun-1996
Externally publishedYes

    Keywords

  • Adult, Cytoplasmic Granules/metabolism, Eosinophils/drug effects, Gene Expression Regulation/drug effects, Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage, Humans, Immunophenotyping, Injections, Subcutaneous, Interleukin-6/metabolism, Interleukin-8/metabolism, Kinetics, L-Selectin/biosynthesis, Lactoferrin/metabolism, Leukocyte Count/drug effects, Leukocyte Elastase, Macrophage-1 Antigen/biosynthesis, Male, Monocytes/drug effects, Neutrophils/drug effects, Pancreatic Elastase/metabolism, Receptors, IgG/biosynthesis, Recombinant Proteins/administration & dosage, Tumor Necrosis Factor-alpha/metabolism

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