Publication

A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS

Berm, E. J. J., Paardekooper, J., Brummel-Mulder, E., Hak, E., Wilffert, B. & Maring, J. G., 1-Mar-2015, In : Talanta. 134, p. 165-172 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Berm, E. J. J., Paardekooper, J., Brummel-Mulder, E., Hak, E., Wilffert, B., & Maring, J. G. (2015). A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS. Talanta, 134, 165-172. https://doi.org/10.1016/j.talanta.2014.10.041

Author

Berm, E. J. J. ; Paardekooper, J. ; Brummel-Mulder, E. ; Hak, E. ; Wilffert, B. ; Maring, J. G. / A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS. In: Talanta. 2015 ; Vol. 134. pp. 165-172.

Harvard

Berm, EJJ, Paardekooper, J, Brummel-Mulder, E, Hak, E, Wilffert, B & Maring, JG 2015, 'A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS' Talanta, vol. 134, pp. 165-172. https://doi.org/10.1016/j.talanta.2014.10.041

Standard

A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS. / Berm, E. J. J.; Paardekooper, J.; Brummel-Mulder, E.; Hak, E.; Wilffert, B.; Maring, J. G.

In: Talanta, Vol. 134, 01.03.2015, p. 165-172.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Berm EJJ, Paardekooper J, Brummel-Mulder E, Hak E, Wilffert B, Maring JG. A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS. Talanta. 2015 Mar 1;134:165-172. https://doi.org/10.1016/j.talanta.2014.10.041


BibTeX

@article{f3d18233b65b4c8aa63e09ed8865225c,
title = "A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS",
abstract = "Therapeutic drug monitoring (TOM) of tricyclic antidepressants (TCAs) is considered useful in patients with major depressive disorder, since these drugs display large individual differences in clearance, and the therapeutic windows of these drugs are relatively small. We developed an assay for determination of amitriptyline (ATP), nortriptyline (NIP), imipramine (IMP), desipramine (DSP) clomipramine (CMP) and desmethyl-clomipramine (DCMP) in dried blood spots (DBS). A fast and robust LC-MS/MS method was developed and analytically validated for simultaneous determination of ATP, NTP, IMP, DSP, CMP, and DCMP in DBS. Six mm circles were punched out from DBS collected on Whatman DMPK-C paper and mixed with acetonitrile: methanol 1:3 containing the internal standard. The extract was analyzed by LC-MS/MS. Total LC-MS/MS runtime was 4.8 min. The assay was linear in the range 20-500 mu g/L, for all compounds. Overall-assay accuracy and precision were <20{\%} for the lower limit of quantification (LLOQ), except for CMP (CV=22.3{\%}), and <15{\%} at other concentrations. The initial LLOQ was 20 mu g/L however for CMP and DMCP it was increased to 40 mu g/L. The blood volume per spot did not influence the results, but a low hematocrit ( 15{\%} negative bias for all compounds. Punching at the perimeter of the blood spot instead of the center was associated with a positive bias. A good correlation was found between patients plasma and DBS samples of ATP, NTP and DMCP, but not for CMP. In addition, proportional differences were found. This LC-MS/MS method was analytically validated for determination of TCAs in DBS. Future validation will focus on the clinical application of the method. (C) 2014 Elsevier B.V. All rights reserved.",
keywords = "Therapeutic drug monitoring, dried blood spot, tricyclic antidepressants, LC-MS/MS, TANDEM MASS-SPECTROMETRY, LIQUID-CHROMATOGRAPHY, CYCLOSPORINE-A, SAMPLES, HEMATOCRIT, DEPRESSION, EFAVIRENZ, SIROLIMUS, PLASMA",
author = "Berm, {E. J. J.} and J. Paardekooper and E. Brummel-Mulder and E. Hak and B. Wilffert and Maring, {J. G.}",
note = "Copyright {\circledC} 2014 Elsevier B.V. All rights reserved.",
year = "2015",
month = "3",
day = "1",
doi = "10.1016/j.talanta.2014.10.041",
language = "English",
volume = "134",
pages = "165--172",
journal = "Talanta",
issn = "0039-9140",
publisher = "ELSEVIER SCIENCE BV",

}

RIS

TY - JOUR

T1 - A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS

AU - Berm, E. J. J.

AU - Paardekooper, J.

AU - Brummel-Mulder, E.

AU - Hak, E.

AU - Wilffert, B.

AU - Maring, J. G.

N1 - Copyright © 2014 Elsevier B.V. All rights reserved.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Therapeutic drug monitoring (TOM) of tricyclic antidepressants (TCAs) is considered useful in patients with major depressive disorder, since these drugs display large individual differences in clearance, and the therapeutic windows of these drugs are relatively small. We developed an assay for determination of amitriptyline (ATP), nortriptyline (NIP), imipramine (IMP), desipramine (DSP) clomipramine (CMP) and desmethyl-clomipramine (DCMP) in dried blood spots (DBS). A fast and robust LC-MS/MS method was developed and analytically validated for simultaneous determination of ATP, NTP, IMP, DSP, CMP, and DCMP in DBS. Six mm circles were punched out from DBS collected on Whatman DMPK-C paper and mixed with acetonitrile: methanol 1:3 containing the internal standard. The extract was analyzed by LC-MS/MS. Total LC-MS/MS runtime was 4.8 min. The assay was linear in the range 20-500 mu g/L, for all compounds. Overall-assay accuracy and precision were <20% for the lower limit of quantification (LLOQ), except for CMP (CV=22.3%), and <15% at other concentrations. The initial LLOQ was 20 mu g/L however for CMP and DMCP it was increased to 40 mu g/L. The blood volume per spot did not influence the results, but a low hematocrit ( 15% negative bias for all compounds. Punching at the perimeter of the blood spot instead of the center was associated with a positive bias. A good correlation was found between patients plasma and DBS samples of ATP, NTP and DMCP, but not for CMP. In addition, proportional differences were found. This LC-MS/MS method was analytically validated for determination of TCAs in DBS. Future validation will focus on the clinical application of the method. (C) 2014 Elsevier B.V. All rights reserved.

AB - Therapeutic drug monitoring (TOM) of tricyclic antidepressants (TCAs) is considered useful in patients with major depressive disorder, since these drugs display large individual differences in clearance, and the therapeutic windows of these drugs are relatively small. We developed an assay for determination of amitriptyline (ATP), nortriptyline (NIP), imipramine (IMP), desipramine (DSP) clomipramine (CMP) and desmethyl-clomipramine (DCMP) in dried blood spots (DBS). A fast and robust LC-MS/MS method was developed and analytically validated for simultaneous determination of ATP, NTP, IMP, DSP, CMP, and DCMP in DBS. Six mm circles were punched out from DBS collected on Whatman DMPK-C paper and mixed with acetonitrile: methanol 1:3 containing the internal standard. The extract was analyzed by LC-MS/MS. Total LC-MS/MS runtime was 4.8 min. The assay was linear in the range 20-500 mu g/L, for all compounds. Overall-assay accuracy and precision were <20% for the lower limit of quantification (LLOQ), except for CMP (CV=22.3%), and <15% at other concentrations. The initial LLOQ was 20 mu g/L however for CMP and DMCP it was increased to 40 mu g/L. The blood volume per spot did not influence the results, but a low hematocrit ( 15% negative bias for all compounds. Punching at the perimeter of the blood spot instead of the center was associated with a positive bias. A good correlation was found between patients plasma and DBS samples of ATP, NTP and DMCP, but not for CMP. In addition, proportional differences were found. This LC-MS/MS method was analytically validated for determination of TCAs in DBS. Future validation will focus on the clinical application of the method. (C) 2014 Elsevier B.V. All rights reserved.

KW - Therapeutic drug monitoring

KW - dried blood spot

KW - tricyclic antidepressants

KW - LC-MS/MS

KW - TANDEM MASS-SPECTROMETRY

KW - LIQUID-CHROMATOGRAPHY

KW - CYCLOSPORINE-A

KW - SAMPLES

KW - HEMATOCRIT

KW - DEPRESSION

KW - EFAVIRENZ

KW - SIROLIMUS

KW - PLASMA

U2 - 10.1016/j.talanta.2014.10.041

DO - 10.1016/j.talanta.2014.10.041

M3 - Article

VL - 134

SP - 165

EP - 172

JO - Talanta

JF - Talanta

SN - 0039-9140

ER -

ID: 15862918