Publication

A sensor for quantification of macromolecular crowding in living cells

Boersma, A. J., Zuhorn, I. S. & Poolman, B., Mar-2015, In : Nature Methods. 12, 3, p. 227-229 3 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • A sensor for quantification of macromolecular crowding in living cells

    Final publisher's version, 740 KB, PDF document

DOI

Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.

Original languageEnglish
Pages (from-to)227-229
Number of pages3
JournalNature Methods
Volume12
Issue number3
Publication statusPublished - Mar-2015

    Keywords

  • PROTEIN STABILITY, LIVE CELLS, IN-VIVO, CYTOPLASM, CONSEQUENCES, CONFINEMENT, DIFFUSION, FICOLL
Related Press / Media
  1. Sensor meet drukte in cel

    Berend Poolman & Arnold Boersma

    28/03/2015

    1 item of Media coverage

    Press/Media: Public Engagement ActivitiesPopular

  2. Groningers meten drukte in cel

    Berend Poolman

    14/02/2015

    1 item of Media coverage

    Press/Media: Public Engagement ActivitiesPopular

View all (2) »

View graph of relations

ID: 16808974