A sensor for quantification of macromolecular crowding in living cellsBoersma, A. J., Zuhorn, I. S. & Poolman, B., Mar-2015, In : Nature Methods. 12, 3, p. 227-229 3 p.
Research output: Contribution to journal › Article › Academic › peer-review
Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.
|Number of pages||3|
|Publication status||Published - Mar-2015|
- PROTEIN STABILITY, LIVE CELLS, IN-VIVO, CYTOPLASM, CONSEQUENCES, CONFINEMENT, DIFFUSION, FICOLL
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