A secreted luciferase for ex vivo monitoring of in vivo processes

Wurdinger, T., Badr, C., Pike, L., de Kleine, R., Weissleder, R., Breakefield, X. O. & Tannous, B. A., Feb-2008, In : Nature Methods. 5, 2, p. 171-3 3 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Thomas Wurdinger
  • Christian Badr
  • Lisa Pike
  • Ruben de Kleine
  • Ralph Weissleder
  • Xandra O Breakefield
  • Bakhos A Tannous

Luciferases are widely used to monitor biological processes. Here we describe the naturally secreted Gaussia princeps luciferase (Gluc) as a highly sensitive reporter for quantitative assessment of cells in vivo by measuring its concentration in blood. The Gluc blood assay complements in vivo bioluminescence imaging, which has the ability to localize the signal and provides a multifaceted assessment of cell viability, proliferation and location in experimental disease and therapy models.

Original languageEnglish
Pages (from-to)171-3
Number of pages3
JournalNature Methods
Issue number2
Publication statusPublished - Feb-2008


  • Animals, Cell Line, Tumor, Fluorescent Dyes, Genes, Reporter, Glioma/metabolism, Humans, Luciferases, Bacterial/genetics, Luminescent Measurements/methods, Magnoliopsida/enzymology, Mice, Mice, Nude, Microscopy, Fluorescence/methods

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