Publication

A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages

Boorsma, C. E., van der Veen, T. A., Putri, K. S. S., de Almeida, A., Draijer, C., Mauad, T., Fejer, G., Brandsma, C-A., van den Berge, M., Bossé, Y., Sin, D., Hao, K., Reithmeier, A., Andersson, G., Olinga, P., Timens, W., Casini, A. & Melgert, B. N., 3-Oct-2017, In : Scientific Reports. 7, 1, 14 p., 12570.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Boorsma, C. E., van der Veen, T. A., Putri, K. S. S., de Almeida, A., Draijer, C., Mauad, T., ... Melgert, B. N. (2017). A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages. Scientific Reports, 7(1), [12570]. https://doi.org/10.1038/s41598-017-12623-w

Author

Boorsma, Carian E ; van der Veen, T. Anienke ; Putri, Kurnia S S ; de Almeida, Andreia ; Draijer, Christina ; Mauad, Thais ; Fejer, Gyorgy ; Brandsma, Corry-Anke ; van den Berge, Maarten ; Bossé, Yohan ; Sin, Don ; Hao, Ke ; Reithmeier, Anja ; Andersson, Göran ; Olinga, Peter ; Timens, Wim ; Casini, Angela ; Melgert, Barbro N. / A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages. In: Scientific Reports. 2017 ; Vol. 7, No. 1.

Harvard

Boorsma, CE, van der Veen, TA, Putri, KSS, de Almeida, A, Draijer, C, Mauad, T, Fejer, G, Brandsma, C-A, van den Berge, M, Bossé, Y, Sin, D, Hao, K, Reithmeier, A, Andersson, G, Olinga, P, Timens, W, Casini, A & Melgert, BN 2017, 'A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages', Scientific Reports, vol. 7, no. 1, 12570. https://doi.org/10.1038/s41598-017-12623-w

Standard

A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages. / Boorsma, Carian E; van der Veen, T. Anienke; Putri, Kurnia S S; de Almeida, Andreia; Draijer, Christina; Mauad, Thais; Fejer, Gyorgy; Brandsma, Corry-Anke; van den Berge, Maarten; Bossé, Yohan; Sin, Don; Hao, Ke; Reithmeier, Anja; Andersson, Göran; Olinga, Peter; Timens, Wim; Casini, Angela; Melgert, Barbro N.

In: Scientific Reports, Vol. 7, No. 1, 12570, 03.10.2017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Boorsma CE, van der Veen TA, Putri KSS, de Almeida A, Draijer C, Mauad T et al. A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages. Scientific Reports. 2017 Oct 3;7(1). 12570. https://doi.org/10.1038/s41598-017-12623-w


BibTeX

@article{709c93695c684e98bd273ba734102e67,
title = "A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages",
abstract = "The enzyme tartrate resistant acid phosphatase (TRAP, two isoforms 5a and 5b) is highly expressed in alveolar macrophages, but its function there is unclear and potent selective inhibitors of TRAP are required to assess functional aspects of the protein. We found higher TRAP activity/expression in lungs of patients with chronic obstructive pulmonary disease (COPD) and asthma compared to controls and more TRAP activity in lungs of mice with experimental COPD or asthma. Stimuli related to asthma and/or COPD were tested for their capacity to induce TRAP. Receptor activator of NF-κb ligand (RANKL) and Xanthine/Xanthine Oxidase induced TRAP mRNA expression in mouse macrophages, but only RANKL also induced TRAP activity in mouse lung slices. Several Au(III) coordination compounds were tested for their ability to inhibit TRAP activity and [Au(4,4'-dimethoxy-2,2'-bipyridine)Cl2][PF6] (AubipyOMe) was found to be the most potent inhibitor of TRAP5a and 5b activity reported to date (IC50 1.3 and 1.8 μM respectively). AubipyOMe also inhibited TRAP activity in murine macrophage and human lung tissue extracts. In a functional assay with physiological TRAP substrate osteopontin, AubipyOMe inhibited mouse macrophage migration over osteopontin-coated membranes. In conclusion, higher TRAP expression/activity are associated with COPD and asthma and TRAP is involved in regulating macrophage migration.",
keywords = "GOLD ORGANOMETALLIC COMPOUNDS, ROS-GENERATING ACTIVITY, DENDRITIC CELLS, COPD PATIENTS, IN-VITRO, GOLD(III) COMPOUNDS, TISSUE MACROPHAGES, PULMONARY-DISEASE, ANTICANCER AGENTS, GENE-EXPRESSION",
author = "Boorsma, {Carian E} and {van der Veen}, {T. Anienke} and Putri, {Kurnia S S} and {de Almeida}, Andreia and Christina Draijer and Thais Mauad and Gyorgy Fejer and Corry-Anke Brandsma and {van den Berge}, Maarten and Yohan Boss{\'e} and Don Sin and Ke Hao and Anja Reithmeier and G{\"o}ran Andersson and Peter Olinga and Wim Timens and Angela Casini and Melgert, {Barbro N}",
year = "2017",
month = "10",
day = "3",
doi = "10.1038/s41598-017-12623-w",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages

AU - Boorsma, Carian E

AU - van der Veen, T. Anienke

AU - Putri, Kurnia S S

AU - de Almeida, Andreia

AU - Draijer, Christina

AU - Mauad, Thais

AU - Fejer, Gyorgy

AU - Brandsma, Corry-Anke

AU - van den Berge, Maarten

AU - Bossé, Yohan

AU - Sin, Don

AU - Hao, Ke

AU - Reithmeier, Anja

AU - Andersson, Göran

AU - Olinga, Peter

AU - Timens, Wim

AU - Casini, Angela

AU - Melgert, Barbro N

PY - 2017/10/3

Y1 - 2017/10/3

N2 - The enzyme tartrate resistant acid phosphatase (TRAP, two isoforms 5a and 5b) is highly expressed in alveolar macrophages, but its function there is unclear and potent selective inhibitors of TRAP are required to assess functional aspects of the protein. We found higher TRAP activity/expression in lungs of patients with chronic obstructive pulmonary disease (COPD) and asthma compared to controls and more TRAP activity in lungs of mice with experimental COPD or asthma. Stimuli related to asthma and/or COPD were tested for their capacity to induce TRAP. Receptor activator of NF-κb ligand (RANKL) and Xanthine/Xanthine Oxidase induced TRAP mRNA expression in mouse macrophages, but only RANKL also induced TRAP activity in mouse lung slices. Several Au(III) coordination compounds were tested for their ability to inhibit TRAP activity and [Au(4,4'-dimethoxy-2,2'-bipyridine)Cl2][PF6] (AubipyOMe) was found to be the most potent inhibitor of TRAP5a and 5b activity reported to date (IC50 1.3 and 1.8 μM respectively). AubipyOMe also inhibited TRAP activity in murine macrophage and human lung tissue extracts. In a functional assay with physiological TRAP substrate osteopontin, AubipyOMe inhibited mouse macrophage migration over osteopontin-coated membranes. In conclusion, higher TRAP expression/activity are associated with COPD and asthma and TRAP is involved in regulating macrophage migration.

AB - The enzyme tartrate resistant acid phosphatase (TRAP, two isoforms 5a and 5b) is highly expressed in alveolar macrophages, but its function there is unclear and potent selective inhibitors of TRAP are required to assess functional aspects of the protein. We found higher TRAP activity/expression in lungs of patients with chronic obstructive pulmonary disease (COPD) and asthma compared to controls and more TRAP activity in lungs of mice with experimental COPD or asthma. Stimuli related to asthma and/or COPD were tested for their capacity to induce TRAP. Receptor activator of NF-κb ligand (RANKL) and Xanthine/Xanthine Oxidase induced TRAP mRNA expression in mouse macrophages, but only RANKL also induced TRAP activity in mouse lung slices. Several Au(III) coordination compounds were tested for their ability to inhibit TRAP activity and [Au(4,4'-dimethoxy-2,2'-bipyridine)Cl2][PF6] (AubipyOMe) was found to be the most potent inhibitor of TRAP5a and 5b activity reported to date (IC50 1.3 and 1.8 μM respectively). AubipyOMe also inhibited TRAP activity in murine macrophage and human lung tissue extracts. In a functional assay with physiological TRAP substrate osteopontin, AubipyOMe inhibited mouse macrophage migration over osteopontin-coated membranes. In conclusion, higher TRAP expression/activity are associated with COPD and asthma and TRAP is involved in regulating macrophage migration.

KW - GOLD ORGANOMETALLIC COMPOUNDS

KW - ROS-GENERATING ACTIVITY

KW - DENDRITIC CELLS

KW - COPD PATIENTS

KW - IN-VITRO

KW - GOLD(III) COMPOUNDS

KW - TISSUE MACROPHAGES

KW - PULMONARY-DISEASE

KW - ANTICANCER AGENTS

KW - GENE-EXPRESSION

U2 - 10.1038/s41598-017-12623-w

DO - 10.1038/s41598-017-12623-w

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 12570

ER -

ID: 48652123