A PCR-aided transcript titration assay revealing very low expression of a gene at band 3p21 in 33 cells lines derived from all types of lung cancer

Kok, K., Buchhagen, D. L., Carritt, B., Buys, C. H. & van den Berg, A., 1993, In : European Journal of Human Genetics. 1, 2, p. 156-63 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

We have developed a general PCR-based method to quantify the amount of a specific mRNA present in a given cell line or tissue. We applied this quantitative PCR to analyse the expression of D8, a human gene which we recently identified in the chromosomal region 3p21, the common deletion region of lung cancer. Our PCR-aided assay shows that in most lung-cancer-derived cell lines the amount of D8 transcripts is only 2% or less of that in normal lung tissue. The virtual absence of expression may imply some role of the gene in the development of lung cancer.

Original languageEnglish
Pages (from-to)156-63
Number of pages8
JournalEuropean Journal of Human Genetics
Issue number2
Publication statusPublished - 1993


  • Base Sequence, Chromosomes, Human, Pair 3, Genes, Tumor Suppressor, Humans, Lung Neoplasms, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Messenger, Tumor Cells, Cultured

ID: 15852401