Publication

A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer

Boichenko, A. P., Govorukhina, N., Klip, H. G., van der Zee, A. G. J., Guezel, C., Luider, T. M. & Bischoff, R., Nov-2014, In : Journal of Proteome Research. 13, 11, p. 4995-5007 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Boichenko, A. P., Govorukhina, N., Klip, H. G., van der Zee, A. G. J., Guezel, C., Luider, T. M., & Bischoff, R. (2014). A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer. Journal of Proteome Research, 13(11), 4995-5007. https://doi.org/10.1021/pr500601w

Author

Boichenko, Alexander P. ; Govorukhina, Natalia ; Klip, Harry G. ; van der Zee, A. G. J. ; Guezel, Coskun ; Luider, Theo M. ; Bischoff, Rainer. / A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 11. pp. 4995-5007.

Harvard

Boichenko, AP, Govorukhina, N, Klip, HG, van der Zee, AGJ, Guezel, C, Luider, TM & Bischoff, R 2014, 'A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer', Journal of Proteome Research, vol. 13, no. 11, pp. 4995-5007. https://doi.org/10.1021/pr500601w

Standard

A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer. / Boichenko, Alexander P.; Govorukhina, Natalia; Klip, Harry G.; van der Zee, A. G. J.; Guezel, Coskun; Luider, Theo M.; Bischoff, Rainer.

In: Journal of Proteome Research, Vol. 13, No. 11, 11.2014, p. 4995-5007.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Boichenko AP, Govorukhina N, Klip HG, van der Zee AGJ, Guezel C, Luider TM et al. A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer. Journal of Proteome Research. 2014 Nov;13(11):4995-5007. https://doi.org/10.1021/pr500601w


BibTeX

@article{d6500afbe81b4d2e850b4d98583744a3,
title = "A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer",
abstract = "We developed a discoveryvalidation mass-spectrometry-based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using iTRAQ, label-free shotgun, and targeted mass-spectrometric quantification. In the discovery stage we used a pooling strategy for the comparative analysis of immunodepleted serum and revealed 15 up- and 26 down-regulated proteins in patients with early- (CES) and late-stage (CLS) cervical cancer. The analysis of nondepleted serum samples from patients with CIN, CES, an CLS and healthy controls showed significant changes in abundance of alpha-1-acid glycoprotein 1, alpha-1-antitrypsin, serotransferrin, haptoglobin, alpha-2-HS-glycoprotein, and vitamin D-binding protein. We validated our findings using a fast UHPLC/MRM method in an independent set of serum samples from patients with cervical cancer or CIN and healthy controls as well as serum samples from patients with ovarian cancer (more than 400 samples in total). The panel of six proteins showed 67% sensitivity and 88% specificity for discrimination of patients with CIN from healthy controls, a stage of the disease where current protein-based biomarkers, for example, squamous cell carcinoma antigen (SCCA), fail to show any discrimination. Additionally, combining the six-protein panel with SCCA improves the discrimination of patients with CES and CLS from healthy controls.",
keywords = "cervical cancer, cervical intraepithelial neoplasia, protein panel, LC-MS/MS, alpha-1-antitrypsin, alpha-1-acid-glycoprotein, haptoglobin, serotransferrin, vitamin D-bidning protein, alpha-2-HS-glycoprotein, SQUAMOUS-CELL CARCINOMA, HIGH-ABUNDANCE PROTEINS, MASS-SPECTROMETRY, PLASMA PROTEOME, TUMOR-MARKERS, SAMPLE PREPARATION, STATISTICAL-MODEL, DATABASE SEARCH, ANTIGEN, HAPTOGLOBIN",
author = "Boichenko, {Alexander P.} and Natalia Govorukhina and Klip, {Harry G.} and {van der Zee}, {A. G. J.} and Coskun Guezel and Luider, {Theo M.} and Rainer Bischoff",
year = "2014",
month = nov,
doi = "10.1021/pr500601w",
language = "English",
volume = "13",
pages = "4995--5007",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "NLM (Medline)",
number = "11",

}

RIS

TY - JOUR

T1 - A Panel of Regulated Proteins in Serum from Patients with Cervical Intraepithelial Neoplasia and Cervical Cancer

AU - Boichenko, Alexander P.

AU - Govorukhina, Natalia

AU - Klip, Harry G.

AU - van der Zee, A. G. J.

AU - Guezel, Coskun

AU - Luider, Theo M.

AU - Bischoff, Rainer

PY - 2014/11

Y1 - 2014/11

N2 - We developed a discoveryvalidation mass-spectrometry-based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using iTRAQ, label-free shotgun, and targeted mass-spectrometric quantification. In the discovery stage we used a pooling strategy for the comparative analysis of immunodepleted serum and revealed 15 up- and 26 down-regulated proteins in patients with early- (CES) and late-stage (CLS) cervical cancer. The analysis of nondepleted serum samples from patients with CIN, CES, an CLS and healthy controls showed significant changes in abundance of alpha-1-acid glycoprotein 1, alpha-1-antitrypsin, serotransferrin, haptoglobin, alpha-2-HS-glycoprotein, and vitamin D-binding protein. We validated our findings using a fast UHPLC/MRM method in an independent set of serum samples from patients with cervical cancer or CIN and healthy controls as well as serum samples from patients with ovarian cancer (more than 400 samples in total). The panel of six proteins showed 67% sensitivity and 88% specificity for discrimination of patients with CIN from healthy controls, a stage of the disease where current protein-based biomarkers, for example, squamous cell carcinoma antigen (SCCA), fail to show any discrimination. Additionally, combining the six-protein panel with SCCA improves the discrimination of patients with CES and CLS from healthy controls.

AB - We developed a discoveryvalidation mass-spectrometry-based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using iTRAQ, label-free shotgun, and targeted mass-spectrometric quantification. In the discovery stage we used a pooling strategy for the comparative analysis of immunodepleted serum and revealed 15 up- and 26 down-regulated proteins in patients with early- (CES) and late-stage (CLS) cervical cancer. The analysis of nondepleted serum samples from patients with CIN, CES, an CLS and healthy controls showed significant changes in abundance of alpha-1-acid glycoprotein 1, alpha-1-antitrypsin, serotransferrin, haptoglobin, alpha-2-HS-glycoprotein, and vitamin D-binding protein. We validated our findings using a fast UHPLC/MRM method in an independent set of serum samples from patients with cervical cancer or CIN and healthy controls as well as serum samples from patients with ovarian cancer (more than 400 samples in total). The panel of six proteins showed 67% sensitivity and 88% specificity for discrimination of patients with CIN from healthy controls, a stage of the disease where current protein-based biomarkers, for example, squamous cell carcinoma antigen (SCCA), fail to show any discrimination. Additionally, combining the six-protein panel with SCCA improves the discrimination of patients with CES and CLS from healthy controls.

KW - cervical cancer

KW - cervical intraepithelial neoplasia

KW - protein panel

KW - LC-MS/MS

KW - alpha-1-antitrypsin

KW - alpha-1-acid-glycoprotein

KW - haptoglobin

KW - serotransferrin

KW - vitamin D-bidning protein

KW - alpha-2-HS-glycoprotein

KW - SQUAMOUS-CELL CARCINOMA

KW - HIGH-ABUNDANCE PROTEINS

KW - MASS-SPECTROMETRY

KW - PLASMA PROTEOME

KW - TUMOR-MARKERS

KW - SAMPLE PREPARATION

KW - STATISTICAL-MODEL

KW - DATABASE SEARCH

KW - ANTIGEN

KW - HAPTOGLOBIN

U2 - 10.1021/pr500601w

DO - 10.1021/pr500601w

M3 - Article

VL - 13

SP - 4995

EP - 5007

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 11

ER -

ID: 14740601