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A Non-Canonical NRPS Is Involved in the Synthesis of Fungisporin and Related Hydrophobic Cyclic Tetrapeptides in Penicillium chrysogenum

Ali, H., Ries, M. I., Lankhorst, P. P., van der Hoeven, R. A. M., Schouten, O. L., Noga, M., Hankemeier, T., van Peij, N. N. M. E., Bovenberg, R. A. L., Vreeken, R. J. & Driessen, A. J. M., 2-Jun-2014, In : PLoS ONE. 9, 6, 10 p., e98212.

Research output: Contribution to journalArticleAcademicpeer-review

The filamentous fungus Penicillium chrysogenum harbors an astonishing variety of nonribosomal peptide synthetase genes, which encode proteins known to produce complex bioactive metabolites from simple building blocks. Here we report a novel non-canonical tetra-modular nonribosomal peptide synthetase (NRPS) with microheterogenicity of all involved adenylation domains towards their respective substrates. By deleting the putative gene in combination with comparative metabolite profiling various unique cyclic and derived linear tetrapeptides were identified which were associated with this NRPS, including fungisporin. In combination with substrate predictions for each module, we propose a mechanism for a 'trans-acting' adenylation domain.

Original languageEnglish
Article numbere98212
Number of pages10
JournalPLoS ONE
Volume9
Issue number6
Publication statusPublished - 2-Jun-2014

    Keywords

  • NONRIBOSOMAL PEPTIDE SYNTHETASES, ASSEMBLY-LINE ENZYMOLOGY, ADENYLATION DOMAIN, YERSINIA-PESTIS, BIOSYNTHESIS, EXPRESSION, PURIFICATION, SPECIFICITY, BIOGENESIS, POLYKETIDE

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