Publication

A nanopore machine promotes the vectorial transport of DNA across membranes

Franceschini, L., Soskine, M., Biesemans, A. & Maglia, G., Sep-2013, In : Nature Communications. 4, p. 1-8 8 p., 2415.

Research output: Contribution to journalArticleAcademicpeer-review

The transport of nucleic acids through membrane pores is a fundamental biological process that occurs in all living organisms. It occurs, for example, during the import of viral DNA into the host cell or during the nuclear pore complex-mediated transport of mRNA in and out the cell nucleus and has implications in nucleic acid drug delivery and gene therapy. Here we describe an engineered DNA transporter that is able to recognize and chaperone a specific DNA molecule across a biological membrane under a fixed transmembrane potential. The transported DNA strand is then released by a simple mechanism based on DNA strand displacement. This nanopore machine might be used to separate or concentrate nucleic acids or to transport genetic information across biological membranes.
Original languageEnglish
Article number2415
Pages (from-to)1-8
Number of pages8
JournalNature Communications
Volume4
Publication statusPublished - Sep-2013
Externally publishedYes

    Keywords

  • single-stranded DNA, protein pore, hybridization

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