Publication

A mouse model for spatial and temporal expression of HGF in the heart

Riess, I., Sala, V., Leo, C., Demaria, M., Gatti, S., Gallo, S., Fitou, A., Boero, O., Levi, R., Cuccovillo, I., Molla, F., De Angelis, N., Staszewsky, L., Latini, R. & Crepaldi, T., Dec-2011, In : Transgenic Research. 20, 6, p. 1203-16 14 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Riess, I., Sala, V., Leo, C., Demaria, M., Gatti, S., Gallo, S., ... Crepaldi, T. (2011). A mouse model for spatial and temporal expression of HGF in the heart. Transgenic Research, 20(6), 1203-16. https://doi.org/10.1007/s11248-011-9485-y

Author

Riess, Ilan ; Sala, Valentina ; Leo, Christian ; Demaria, Marco ; Gatti, Stefano ; Gallo, Simona ; Fitou, Amandine ; Boero, Ombretta ; Levi, Renzo ; Cuccovillo, Ivan ; Molla, Fabiola ; De Angelis, Noeleen ; Staszewsky, Lidia ; Latini, Roberto ; Crepaldi, Tiziana. / A mouse model for spatial and temporal expression of HGF in the heart. In: Transgenic Research. 2011 ; Vol. 20, No. 6. pp. 1203-16.

Harvard

Riess, I, Sala, V, Leo, C, Demaria, M, Gatti, S, Gallo, S, Fitou, A, Boero, O, Levi, R, Cuccovillo, I, Molla, F, De Angelis, N, Staszewsky, L, Latini, R & Crepaldi, T 2011, 'A mouse model for spatial and temporal expression of HGF in the heart' Transgenic Research, vol. 20, no. 6, pp. 1203-16. https://doi.org/10.1007/s11248-011-9485-y

Standard

A mouse model for spatial and temporal expression of HGF in the heart. / Riess, Ilan; Sala, Valentina; Leo, Christian; Demaria, Marco; Gatti, Stefano; Gallo, Simona; Fitou, Amandine; Boero, Ombretta; Levi, Renzo; Cuccovillo, Ivan; Molla, Fabiola; De Angelis, Noeleen; Staszewsky, Lidia; Latini, Roberto; Crepaldi, Tiziana.

In: Transgenic Research, Vol. 20, No. 6, 12.2011, p. 1203-16.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Riess I, Sala V, Leo C, Demaria M, Gatti S, Gallo S et al. A mouse model for spatial and temporal expression of HGF in the heart. Transgenic Research. 2011 Dec;20(6):1203-16. https://doi.org/10.1007/s11248-011-9485-y


BibTeX

@article{1e93d157bb56453c8883de1ff2a20d0a,
title = "A mouse model for spatial and temporal expression of HGF in the heart",
abstract = "In order to study the effects of Hepatocyte Growth Factor (HGF) in the heart, two transgenic mice were developed, one carrying a bidirectional HGF-TetO-GFP responder construct and the other carrying a α-MHC-tTA transactivator construct. Crosses were carried out between heterozygotes, so that litters contained bitransgenic α-MHC-tTA/HGF-TetO-GFP+, thus expressing HGF and GFP exclusively in the heart and only in the absence of Doxycycline. Our data show that the expression of HGF was indeed restricted to the heart and that the expression was limited to the timeframe of the absence of Doxycycline. Surprisingly the expression was variable even between bitransgenic littermates. In the setting of a model of ischemia-reperfusion, the expression of HGF ameliorates cardiac functionality, enhances proliferation and diminishes the scarred area, proving that this is a good model to study the beneficial influences and functional roles of HGF in the heart.",
keywords = "Animals, Blotting, Western, Cell Line, Cell Proliferation, Collagen, Crosses, Genetic, Culture Media, Conditioned, Dogs, Doxycycline, Echocardiography, Female, Gene Expression Regulation, Green Fluorescent Proteins, Heart, Hepatocyte Growth Factor, Heterozygote, Immunohistochemistry, Mice, Mice, Transgenic, Models, Animal, Myocardial Reperfusion Injury, Pregnancy, Real-Time Polymerase Chain Reaction",
author = "Ilan Riess and Valentina Sala and Christian Leo and Marco Demaria and Stefano Gatti and Simona Gallo and Amandine Fitou and Ombretta Boero and Renzo Levi and Ivan Cuccovillo and Fabiola Molla and {De Angelis}, Noeleen and Lidia Staszewsky and Roberto Latini and Tiziana Crepaldi",
year = "2011",
month = "12",
doi = "10.1007/s11248-011-9485-y",
language = "English",
volume = "20",
pages = "1203--16",
journal = "Transgenic Research",
issn = "0962-8819",
number = "6",

}

RIS

TY - JOUR

T1 - A mouse model for spatial and temporal expression of HGF in the heart

AU - Riess, Ilan

AU - Sala, Valentina

AU - Leo, Christian

AU - Demaria, Marco

AU - Gatti, Stefano

AU - Gallo, Simona

AU - Fitou, Amandine

AU - Boero, Ombretta

AU - Levi, Renzo

AU - Cuccovillo, Ivan

AU - Molla, Fabiola

AU - De Angelis, Noeleen

AU - Staszewsky, Lidia

AU - Latini, Roberto

AU - Crepaldi, Tiziana

PY - 2011/12

Y1 - 2011/12

N2 - In order to study the effects of Hepatocyte Growth Factor (HGF) in the heart, two transgenic mice were developed, one carrying a bidirectional HGF-TetO-GFP responder construct and the other carrying a α-MHC-tTA transactivator construct. Crosses were carried out between heterozygotes, so that litters contained bitransgenic α-MHC-tTA/HGF-TetO-GFP+, thus expressing HGF and GFP exclusively in the heart and only in the absence of Doxycycline. Our data show that the expression of HGF was indeed restricted to the heart and that the expression was limited to the timeframe of the absence of Doxycycline. Surprisingly the expression was variable even between bitransgenic littermates. In the setting of a model of ischemia-reperfusion, the expression of HGF ameliorates cardiac functionality, enhances proliferation and diminishes the scarred area, proving that this is a good model to study the beneficial influences and functional roles of HGF in the heart.

AB - In order to study the effects of Hepatocyte Growth Factor (HGF) in the heart, two transgenic mice were developed, one carrying a bidirectional HGF-TetO-GFP responder construct and the other carrying a α-MHC-tTA transactivator construct. Crosses were carried out between heterozygotes, so that litters contained bitransgenic α-MHC-tTA/HGF-TetO-GFP+, thus expressing HGF and GFP exclusively in the heart and only in the absence of Doxycycline. Our data show that the expression of HGF was indeed restricted to the heart and that the expression was limited to the timeframe of the absence of Doxycycline. Surprisingly the expression was variable even between bitransgenic littermates. In the setting of a model of ischemia-reperfusion, the expression of HGF ameliorates cardiac functionality, enhances proliferation and diminishes the scarred area, proving that this is a good model to study the beneficial influences and functional roles of HGF in the heart.

KW - Animals

KW - Blotting, Western

KW - Cell Line

KW - Cell Proliferation

KW - Collagen

KW - Crosses, Genetic

KW - Culture Media, Conditioned

KW - Dogs

KW - Doxycycline

KW - Echocardiography

KW - Female

KW - Gene Expression Regulation

KW - Green Fluorescent Proteins

KW - Heart

KW - Hepatocyte Growth Factor

KW - Heterozygote

KW - Immunohistochemistry

KW - Mice

KW - Mice, Transgenic

KW - Models, Animal

KW - Myocardial Reperfusion Injury

KW - Pregnancy

KW - Real-Time Polymerase Chain Reaction

U2 - 10.1007/s11248-011-9485-y

DO - 10.1007/s11248-011-9485-y

M3 - Article

VL - 20

SP - 1203

EP - 1216

JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

IS - 6

ER -

ID: 26938304