Publication

A modular yeast biosensor for low-cost point-of-care pathogen detection

Ostrov, N., Jimenez, M., Billerbeck, S., Brisbois, J., Matragrano, J., Ager, A. & Cornish, V. W., Jun-2017, In : Science Advances. 3, 6, 9 p., e1603221.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Ostrov, N., Jimenez, M., Billerbeck, S., Brisbois, J., Matragrano, J., Ager, A., & Cornish, V. W. (2017). A modular yeast biosensor for low-cost point-of-care pathogen detection. Science Advances, 3(6), [e1603221]. https://doi.org/10.1126/sciadv.1603221

Author

Ostrov, Nili ; Jimenez, Miguel ; Billerbeck, Sonja ; Brisbois, James ; Matragrano, Joseph ; Ager, Alastair ; Cornish, Virginia W. / A modular yeast biosensor for low-cost point-of-care pathogen detection. In: Science Advances. 2017 ; Vol. 3, No. 6.

Harvard

Ostrov, N, Jimenez, M, Billerbeck, S, Brisbois, J, Matragrano, J, Ager, A & Cornish, VW 2017, 'A modular yeast biosensor for low-cost point-of-care pathogen detection', Science Advances, vol. 3, no. 6, e1603221. https://doi.org/10.1126/sciadv.1603221

Standard

A modular yeast biosensor for low-cost point-of-care pathogen detection. / Ostrov, Nili; Jimenez, Miguel; Billerbeck, Sonja; Brisbois, James; Matragrano, Joseph; Ager, Alastair; Cornish, Virginia W.

In: Science Advances, Vol. 3, No. 6, e1603221, 06.2017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Ostrov N, Jimenez M, Billerbeck S, Brisbois J, Matragrano J, Ager A et al. A modular yeast biosensor for low-cost point-of-care pathogen detection. Science Advances. 2017 Jun;3(6). e1603221. https://doi.org/10.1126/sciadv.1603221


BibTeX

@article{f1dbdc0af7894def8f5c390402a47d8c,
title = "A modular yeast biosensor for low-cost point-of-care pathogen detection",
abstract = "The availability of simple, specific, and inexpensive on-site detection methods is of key importance for deployment of pathogen surveillance networks. We developed a nontechnical and highly specific colorimetric assay for detection of pathogen-derived peptides based on Saccharomyces cerevisiae-a genetically tractable model organism and household product. Integrating G protein-coupled receptors with a visible, reagent-free lycopene readout, we demonstrate differential detection of major human, plant, and food fungal pathogens with nanomolar sensitivity. We further optimized a one-step rapid dipstick prototype that can be used in complex samples, including blood, urine, and soil. This modular biosensor can be economically produced at large scale, is not reliant on cold-chain storage, can be detected without additional equipment, and is thus a compelling platform scalable to global surveillance of pathogens.",
author = "Nili Ostrov and Miguel Jimenez and Sonja Billerbeck and James Brisbois and Joseph Matragrano and Alastair Ager and Cornish, {Virginia W}",
year = "2017",
month = "6",
doi = "10.1126/sciadv.1603221",
language = "English",
volume = "3",
journal = "Science Advances",
issn = "2375-2548",
publisher = "AMER ASSOC ADVANCEMENT SCIENCE",
number = "6",

}

RIS

TY - JOUR

T1 - A modular yeast biosensor for low-cost point-of-care pathogen detection

AU - Ostrov, Nili

AU - Jimenez, Miguel

AU - Billerbeck, Sonja

AU - Brisbois, James

AU - Matragrano, Joseph

AU - Ager, Alastair

AU - Cornish, Virginia W

PY - 2017/6

Y1 - 2017/6

N2 - The availability of simple, specific, and inexpensive on-site detection methods is of key importance for deployment of pathogen surveillance networks. We developed a nontechnical and highly specific colorimetric assay for detection of pathogen-derived peptides based on Saccharomyces cerevisiae-a genetically tractable model organism and household product. Integrating G protein-coupled receptors with a visible, reagent-free lycopene readout, we demonstrate differential detection of major human, plant, and food fungal pathogens with nanomolar sensitivity. We further optimized a one-step rapid dipstick prototype that can be used in complex samples, including blood, urine, and soil. This modular biosensor can be economically produced at large scale, is not reliant on cold-chain storage, can be detected without additional equipment, and is thus a compelling platform scalable to global surveillance of pathogens.

AB - The availability of simple, specific, and inexpensive on-site detection methods is of key importance for deployment of pathogen surveillance networks. We developed a nontechnical and highly specific colorimetric assay for detection of pathogen-derived peptides based on Saccharomyces cerevisiae-a genetically tractable model organism and household product. Integrating G protein-coupled receptors with a visible, reagent-free lycopene readout, we demonstrate differential detection of major human, plant, and food fungal pathogens with nanomolar sensitivity. We further optimized a one-step rapid dipstick prototype that can be used in complex samples, including blood, urine, and soil. This modular biosensor can be economically produced at large scale, is not reliant on cold-chain storage, can be detected without additional equipment, and is thus a compelling platform scalable to global surveillance of pathogens.

U2 - 10.1126/sciadv.1603221

DO - 10.1126/sciadv.1603221

M3 - Article

VL - 3

JO - Science Advances

JF - Science Advances

SN - 2375-2548

IS - 6

M1 - e1603221

ER -

ID: 86478980